PMID- 33651947
OWN - NLM
STAT- MEDLINE
DCOM- 20210802
LR  - 20210802
IS  - 2373-9878 (Electronic)
IS  - 2373-9878 (Linking)
VI  - 7
IP  - 7
DP  - 2021 Jul 12
TI  - Mechanical Properties of Soft Biological Membranes for Organ-on-a-Chip Assessed 
      by Bulge Test and AFM.
PG  - 2990-2997
LID - 10.1021/acsbiomaterials.0c00515 [doi]
AB  - Advanced in vitro models called "organ-on-a-chip" can mimic the specific cellular 
      environment found in various tissues. Many of these models include a thin, 
      sometimes flexible, membrane aimed at mimicking the extracellular matrix (ECM) 
      scaffold of in vivo barriers. These membranes are often made of 
      polydimethylsiloxane (PDMS), a silicone rubber that poorly mimics the chemical 
      and physical properties of the basal membrane. However, the ECM and its 
      mechanical properties play a key role in the homeostasis of a tissue. Here, we 
      report about biological membranes with a composition and mechanical properties 
      similar to those found in vivo. Two types of collagen-elastin (CE) membranes were 
      produced: vitrified and nonvitrified (called "hydrogel membrane"). Their 
      mechanical properties were characterized using the bulge test method. The results 
      were compared using atomic force microscopy (AFM), a standard technique used to 
      evaluate the Young's modulus of soft materials at the nanoscale. Our results show 
      that CE membranes with stiffnesses ranging from several hundred of kPa down to 1 
      kPa can be produced by tuning the CE ratio, the production mode (vitrified or 
      not), and/or certain parameters such as temperature. The Young's modulus can 
      easily be determined using the bulge test. This method is a robust and 
      reproducible to determine membrane stiffness, even for soft membranes, which are 
      more difficult to assess by AFM. Assessment of the impact of substrate stiffness 
      on the spread of human fibroblasts on these surfaces showed that cell spread is 
      lower on softer surfaces than on stiffer surfaces.
FAU - Zamprogno, Pauline
AU  - Zamprogno P
AUID- ORCID: 0000-0003-1068-7304
AD  - Organs-on-Chip Technologies Laboratory, ARTORG Center, University of Bern, Bern 
      3008, Switzerland.
FAU - Thoma, Giuditta
AU  - Thoma G
AD  - Organs-on-Chip Technologies Laboratory, ARTORG Center, University of Bern, Bern 
      3008, Switzerland.
FAU - Cencen, Veronika
AU  - Cencen V
AD  - Laboratory for Bio- and Nano- Instrumentation, Ecole Polytechnique Federale de 
      Lausanne, Lausanne 1015, Switzerland.
FAU - Ferrari, Dario
AU  - Ferrari D
AD  - Organs-on-Chip Technologies Laboratory, ARTORG Center, University of Bern, Bern 
      3008, Switzerland.
FAU - Putz, Barbara
AU  - Putz B
AD  - Laboratory for Mechanics of Materials and Nanostructures, EMPA Swiss Federal 
      Laboratories for Materials Science and Technology, Thun 3602, Switzerland.
FAU - Michler, Johann
AU  - Michler J
AD  - Laboratory for Mechanics of Materials and Nanostructures, EMPA Swiss Federal 
      Laboratories for Materials Science and Technology, Thun 3602, Switzerland.
FAU - Fantner, Georg E
AU  - Fantner GE
AD  - Laboratory for Bio- and Nano- Instrumentation, Ecole Polytechnique Federale de 
      Lausanne, Lausanne 1015, Switzerland.
FAU - Guenat, Olivier T
AU  - Guenat OT
AD  - Organs-on-Chip Technologies Laboratory, ARTORG Center, University of Bern, Bern 
      3008, Switzerland.
AD  - Department of Pulmonary Medicine, University Hospital of Bern, Bern 3008, 
      Switzerland.
AD  - Department of General Thoracic Surgery, University Hospital of Bern, Bern 3008, 
      Switzerland.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210302
PL  - United States
TA  - ACS Biomater Sci Eng
JT  - ACS biomaterials science & engineering
JID - 101654670
SB  - IM
MH  - Cell Membrane
MH  - Elastic Modulus
MH  - *Extracellular Matrix
MH  - Humans
MH  - *Lab-On-A-Chip Devices
MH  - Microscopy, Atomic Force
OTO - NOTNLM
OT  - ECM membrane
OT  - Young's modulus
OT  - atomic force microscopy
OT  - bulge test
OT  - organ-on-a-chip
EDAT- 2021/03/03 06:00
MHDA- 2021/08/03 06:00
CRDT- 2021/03/02 20:08
PHST- 2021/03/03 06:00 [pubmed]
PHST- 2021/08/03 06:00 [medline]
PHST- 2021/03/02 20:08 [entrez]
AID - 10.1021/acsbiomaterials.0c00515 [doi]
PST - ppublish
SO  - ACS Biomater Sci Eng. 2021 Jul 12;7(7):2990-2997. doi: 
      10.1021/acsbiomaterials.0c00515. Epub 2021 Mar 2.