PMID- 33652180
OWN - NLM
STAT- MEDLINE
DCOM- 20210413
LR  - 20220531
IS  - 1873-6424 (Electronic)
IS  - 0269-7491 (Linking)
VI  - 277
DP  - 2021 May 15
TI  - Third-hand smoke exposure is associated with abnormal serum melatonin level via 
      hypomethylation of CYP1A2 promoter: Evidence from human and animal studies.
PG  - 116669
LID - S0269-7491(21)00247-5 [pii]
LID - 10.1016/j.envpol.2021.116669 [doi]
AB  - This study aimed to examine whether and how third-hand smoke (THS) exposure would 
      influence serum melatonin level. 1083 participants with or without exposure to 
      THS were enrolled. Serum ROS, SOD, GSH-Px, and melatonin were measured by ELISA. 
      Methylation microarrays detection and WGCNA were performed to identify hub 
      methylated-sites. The methylation levels of hub-sites were validated in addtional 
      samples. Moreover, mice were exposed to THS for 6 months mimicking exposure of 
      human and the serum, liver, and pineal were collected. Oxidative stress-related 
      indicators in serum, pineal, and liver were measured by ELISA. The expressions of 
      mRNA and protein and methylation levels of hub-gene discovered in human data were 
      further explored by RT-PCR, western-blot, and TBS. The results showed the 
      participants exposed to THS had lower melatonin-level. 820 differentially 
      methylated sites associated with THS were identified. And the hub-site located on 
      the CYP1A2 promoter was identified, which mediated the association between THS 
      and decreased melatonin-level. Decreased peak of serum melatonin, increased ROS 
      and reduced SOD and GSH-Px in pineal and liver, and elevated CYP1A2 expression in 
      liver was also found in the THS-exposed mice. Hypo-methylation of 7 CPG sites on 
      the CYP1A2 promoter was identified, which accelerated the catabolism of 
      melatonin. Overall, THS exposure is associated with abnormal melatonin catabolism 
      through hypo-methylation of CYP1A2-promoter.
CI  - Copyright (c) 2021 Elsevier Ltd. All rights reserved.
FAU - Jiang, Wenbo
AU  - Jiang W
AD  - Department of Nutrition and Food Hygiene, School of Public Health, Harbin Medical 
      University, Harbin, Heilongjiang Province 150081, P. R. China.
FAU - Wu, Huanyu
AU  - Wu H
AD  - Department of Nutrition and Food Hygiene, School of Public Health, Harbin Medical 
      University, Harbin, Heilongjiang Province 150081, P. R. China.
FAU - Yu, Xinyang
AU  - Yu X
AD  - Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical 
      College, Beijing, China.
FAU - Wang, Yu
AU  - Wang Y
AD  - Department of Nutrition and Food Hygiene, School of Public Health, Harbin Medical 
      University, Harbin, Heilongjiang Province 150081, P. R. China.
FAU - Gu, Wenbo
AU  - Gu W
AD  - Department of Nutrition and Food Hygiene, School of Public Health, Harbin Medical 
      University, Harbin, Heilongjiang Province 150081, P. R. China.
FAU - Wei, Wei
AU  - Wei W
AD  - Department of Nutrition and Food Hygiene, School of Public Health, Harbin Medical 
      University, Harbin, Heilongjiang Province 150081, P. R. China.
FAU - Li, Bai
AU  - Li B
AD  - University of Ottawa, Ottawa K1N 6N5, Canada.
FAU - Jiang, XiTao
AU  - Jiang X
AD  - IT and Environment, College of Engineering, Charles Darwin University, Darwin, 
      Northern Territory 0810, Australia.
FAU - Wang, Yue
AU  - Wang Y
AD  - Department of Nutrition and Food Hygiene, School of Public Health, Harbin Medical 
      University, Harbin, Heilongjiang Province 150081, P. R. China.
FAU - Hou, Wanying
AU  - Hou W
AD  - Department of Nutrition and Food Hygiene, School of Public Health, Harbin Medical 
      University, Harbin, Heilongjiang Province 150081, P. R. China.
FAU - Dong, Qiuying
AU  - Dong Q
AD  - Department of Nutrition and Food Hygiene, School of Public Health, Harbin Medical 
      University, Harbin, Heilongjiang Province 150081, P. R. China.
FAU - Yan, Xuemin
AU  - Yan X
AD  - Department of Nutrition and Food Hygiene, School of Public Health, Harbin Medical 
      University, Harbin, Heilongjiang Province 150081, P. R. China.
FAU - Li, Ying
AU  - Li Y
AD  - Department of Nutrition and Food Hygiene, School of Public Health, Harbin Medical 
      University, Harbin, Heilongjiang Province 150081, P. R. China.
FAU - Sun, Changhao
AU  - Sun C
AD  - Department of Nutrition and Food Hygiene, School of Public Health, Harbin Medical 
      University, Harbin, Heilongjiang Province 150081, P. R. China.
FAU - Han, Tianshu
AU  - Han T
AD  - Department of Nutrition and Food Hygiene, School of Public Health, Harbin Medical 
      University, Harbin, Heilongjiang Province 150081, P. R. China. Electronic 
      address: hmutianshuhan@163.com.
LA  - eng
PT  - Journal Article
DEP - 20210223
PL  - England
TA  - Environ Pollut
JT  - Environmental pollution (Barking, Essex : 1987)
JID - 8804476
RN  - 0 (Smoke)
RN  - EC 1.14.14.1 (CYP1A2 protein, human)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP1A2)
RN  - JL5DK93RCL (Melatonin)
SB  - IM
MH  - Animals
MH  - Cytochrome P-450 CYP1A2/genetics
MH  - DNA Methylation
MH  - Humans
MH  - *Melatonin
MH  - Mice
MH  - Promoter Regions, Genetic
MH  - Smoke
OTO - NOTNLM
OT  - DNA methylation
OT  - Melatonin
OT  - Third-hand smoke
OT  - WGCNA
COIS- Declaration of competing interest The authors have declared that no conflicts of 
      interest exist.
EDAT- 2021/03/03 06:00
MHDA- 2021/04/14 06:00
CRDT- 2021/03/02 20:09
PHST- 2020/06/12 00:00 [received]
PHST- 2021/01/08 00:00 [revised]
PHST- 2021/02/01 00:00 [accepted]
PHST- 2021/03/03 06:00 [pubmed]
PHST- 2021/04/14 06:00 [medline]
PHST- 2021/03/02 20:09 [entrez]
AID - S0269-7491(21)00247-5 [pii]
AID - 10.1016/j.envpol.2021.116669 [doi]
PST - ppublish
SO  - Environ Pollut. 2021 May 15;277:116669. doi: 10.1016/j.envpol.2021.116669. Epub 
      2021 Feb 23.