PMID- 33652966
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210413
IS  - 2076-2607 (Print)
IS  - 2076-2607 (Electronic)
IS  - 2076-2607 (Linking)
VI  - 9
IP  - 3
DP  - 2021 Feb 26
TI  - Diguanylate Cyclase GdpX6 with c-di-GMP Binding Activity Involved in the 
      Regulation of Virulence Expression in Xanthomonas oryzae pv. oryzae.
LID - 10.3390/microorganisms9030495 [doi]
LID - 495
AB  - Cyclic diguanylate monophosphate (c-di-GMP) is a secondary messenger present in 
      bacteria. The GGDEF-domain proteins can participate in the synthesis of c-di-GMP 
      as diguanylate cyclase (DGC) or bind with c-di-GMP to function as a c-di-GMP 
      receptor. In the genome of Xanthomonas oryzae pv. oryzae (Xoo), the causal agent 
      of bacterial blight of rice, there are 11 genes that encode single GGDEF domain 
      proteins. The GGDEF domain protein, PXO_02019 (here GdpX6 [GGDEF-domain protein 
      of Xoo6]) was characterized in the present study. Firstly, the DGC and c-di-GMP 
      binding activity of GdpX6 was confirmed in vitro. Mutation of the crucial 
      residues D(403) residue of the I site in GGDEF motif and E(411) residue of A site 
      in GGDEF motif of GdpX6 abolished c-di-GMP binding activity and DGC activity of 
      GdpX6, respectively. Additionally, deletion of gdpX6 significantly increased the 
      virulence, swimming motility, and decreased sliding motility and biofilm 
      formation. In contrast, overexpression of GdpX6 in wild-type PXO99(A) strain 
      decreased the virulence and swimming motility, and increased sliding motility and 
      biofilm formation. Mutation of the E(411) residue but not D(403) residue of the 
      GGDEF domain in GdpX6 abolished its biological functions, indicating the DGC 
      activity to be imperative for its biological functions. Furthermore, GdpX6 
      exhibited multiple subcellular localization in bacterial cells, and D(403) or 
      E(411) did not contribute to the localization of GdpX6. Thus, we concluded that 
      GdpX6 exhibits DGC activity to control the virulence, swimming and sliding 
      motility, and biofilm formation in Xoo.
FAU - Yan, Weiwei
AU  - Yan W
AD  - State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of 
      Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, 
      China.
AD  - The MOA Key Laboratory of Plant Pathology, Department of Plant Pathology, College 
      of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, 
      China.
FAU - Wei, Yiming
AU  - Wei Y
AD  - State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of 
      Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, 
      China.
FAU - Fan, Susu
AU  - Fan S
AD  - Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, 
      Shandong Academy of Sciences, Jinan 250014, China.
FAU - Yu, Chao
AU  - Yu C
AD  - State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of 
      Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, 
      China.
FAU - Tian, Fang
AU  - Tian F
AD  - State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of 
      Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, 
      China.
FAU - Wang, Qi
AU  - Wang Q
AD  - The MOA Key Laboratory of Plant Pathology, Department of Plant Pathology, College 
      of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, 
      China.
FAU - Yang, Fenghuan
AU  - Yang F
AD  - State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of 
      Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, 
      China.
FAU - Chen, Huamin
AU  - Chen H
AD  - State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of 
      Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, 
      China.
LA  - eng
GR  - 31671990 and 31400117/National Natural Science Foundation of China/
GR  - 2016YFD0300701/National Key R&D Program of China/
PT  - Journal Article
DEP - 20210226
PL  - Switzerland
TA  - Microorganisms
JT  - Microorganisms
JID - 101625893
PMC - PMC7996900
OTO - NOTNLM
OT  - GGDEF domain 5
OT  - Xanthomonas oryzae pv. oryzae 1
OT  - c-di-GMP 3
OT  - diguanylate cyclase 4
OT  - virulence 2
COIS- The authors declare no conflict of interest.
EDAT- 2021/03/04 06:00
MHDA- 2021/03/04 06:01
PMCR- 2021/02/26
CRDT- 2021/03/03 01:02
PHST- 2021/01/28 00:00 [received]
PHST- 2021/02/19 00:00 [revised]
PHST- 2021/02/23 00:00 [accepted]
PHST- 2021/03/03 01:02 [entrez]
PHST- 2021/03/04 06:00 [pubmed]
PHST- 2021/03/04 06:01 [medline]
PHST- 2021/02/26 00:00 [pmc-release]
AID - microorganisms9030495 [pii]
AID - microorganisms-09-00495 [pii]
AID - 10.3390/microorganisms9030495 [doi]
PST - epublish
SO  - Microorganisms. 2021 Feb 26;9(3):495. doi: 10.3390/microorganisms9030495.