PMID- 33654287
OWN - NLM
STAT- MEDLINE
DCOM- 20220325
LR  - 20221026
IS  - 1530-0447 (Electronic)
IS  - 0031-3998 (Print)
IS  - 0031-3998 (Linking)
VI  - 90
IP  - 6
DP  - 2021 Dec
TI  - Altered microbiomes in thirdhand smoke-exposed children and their home 
      environments.
PG  - 1153-1160
LID - 10.1038/s41390-021-01400-1 [doi]
AB  - INTRODUCTION: Tobacco smoke contains numerous toxic chemicals that accumulate in 
      indoor environments creating thirdhand smoke (THS). We investigated if 
      THS-polluted homes differed in children's human and built-environment microbiomes 
      as compared to THS-free homes. METHODS: Participants were n = 19 THS-exposed 
      children and n = 10 unexposed children (</=5 years) and their caregivers. 
      Environmental and biological samples were analyzed for THS pollutants and 
      exposure. Swab samples were collected from the built-environment (floor, table, 
      armrest, bed frame) and child (finger, nose, mouth, and ear canal), and 16S 
      ribosomal RNA genes were analyzed for bacterial taxa using high-throughput DNA 
      sequencing. RESULTS: Phylogenetic alpha-diversity was significantly higher for the 
      built-environment microbiomes in THS-polluted homes compared to THS-free homes 
      (p < 0.014). Log2-fold comparison found differences between THS-polluted and 
      THS-free homes for specific genera in samples from the built-environment (e.g., 
      Acinetobacter, Bradyrhizobium, Corynebacterium, Gemella, Neisseria, 
      Staphylococcus, Streptococcus, and Veillonella) and in samples from children 
      (esp. Corynebacterium, Gemella, Lautropia, Neisseria, Rothia, Staphylococcus, and 
      Veillonella). CONCLUSION: When exposed to THS, indoor and children microbiomes 
      are altered in an environment-specific manner. Changes are similar to those 
      reported in previous studies for smokers and secondhand smoke-exposed persons. 
      THS-induced changes in child and built-environmental microbiomes may play a role 
      in clinical outcomes in children. IMPACT: Despite smoking bans, children can be 
      exposed to tobacco smoke residue (i.e., thirdhand smoke) that lingers on surfaces 
      and in settled house dust. Thirdhand smoke exposure is associated with changes in 
      the microbiomes of the home environment and of the children living in these 
      homes. Thirdhand smoke is associated with increased phylogenetic diversity of the 
      home environment and changes in the abundances of several genera of the child 
      microbiome known to be affected by active smoking and secondhand smoke (e.g., 
      Corynebacterium, Staphylococcus, Streptococcus). Thirdhand smoke exposure by 
      itself may induce alterations in the microbiome that play a role in childhood 
      pathologies.
CI  - (c) 2021. The Author(s), under exclusive licence to the International Pediatric 
      Research Foundation, Inc.
FAU - Kelley, Scott T
AU  - Kelley ST
AD  - San Diego State University, San Diego, CA, USA.
FAU - Liu, William
AU  - Liu W
AD  - San Diego State University, San Diego, CA, USA.
FAU - Quintana, Penelope J E
AU  - Quintana PJE
AD  - San Diego State University, San Diego, CA, USA.
FAU - Hoh, Eunha
AU  - Hoh E
AD  - San Diego State University, San Diego, CA, USA.
FAU - Dodder, Nathan G
AU  - Dodder NG
AD  - San Diego State University, San Diego, CA, USA.
FAU - Mahabee-Gittens, E Melinda
AU  - Mahabee-Gittens EM
AD  - Cincinnati Children's Hospital Medical Center, University of Cincinnati College 
      of Medicine, Cincinnati, OH, USA.
FAU - Padilla, Samuel
AU  - Padilla S
AD  - San Diego State University, San Diego, CA, USA.
FAU - Ogden, Shawn
AU  - Ogden S
AD  - San Diego State University, San Diego, CA, USA.
FAU - Frenzel, Sia
AU  - Frenzel S
AD  - San Diego State University, San Diego, CA, USA.
FAU - Sisk-Hackworth, Laura
AU  - Sisk-Hackworth L
AD  - San Diego State University, San Diego, CA, USA.
FAU - Matt, Georg E
AU  - Matt GE
AD  - San Diego State University, San Diego, CA, USA. gmatt@sdsu.edu.
LA  - eng
GR  - R01 ES027815/ES/NIEHS NIH HHS/United States
GR  - R01 ES030743/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20210302
PL  - United States
TA  - Pediatr Res
JT  - Pediatric research
JID - 0100714
RN  - 0 (Tobacco Smoke Pollution)
SB  - IM
MH  - Bacteria/classification
MH  - Child, Preschool
MH  - *Family Characteristics
MH  - Humans
MH  - *Microbiota
MH  - Species Specificity
MH  - *Tobacco Smoke Pollution
PMC - PMC8410873
MID - NIHMS1667947
COIS- Declaration of Interests The authors declare no competing interests.
EDAT- 2021/03/04 06:00
MHDA- 2022/03/26 06:00
PMCR- 2021/12/17
CRDT- 2021/03/03 05:45
PHST- 2020/10/26 00:00 [received]
PHST- 2021/01/06 00:00 [accepted]
PHST- 2020/12/29 00:00 [revised]
PHST- 2021/03/04 06:00 [pubmed]
PHST- 2022/03/26 06:00 [medline]
PHST- 2021/03/03 05:45 [entrez]
PHST- 2021/12/17 00:00 [pmc-release]
AID - 10.1038/s41390-021-01400-1 [pii]
AID - 10.1038/s41390-021-01400-1 [doi]
PST - ppublish
SO  - Pediatr Res. 2021 Dec;90(6):1153-1160. doi: 10.1038/s41390-021-01400-1. Epub 2021 
      Mar 2.