PMID- 33654672
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220421
IS  - 2223-4292 (Print)
IS  - 2223-4306 (Electronic)
IS  - 2223-4306 (Linking)
VI  - 11
IP  - 3
DP  - 2021 Mar
TI  - Optical imaging of stimulation-evoked cortical activity using GCaMP6f and 
      jRGECO1a.
PG  - 998-1009
LID - 10.21037/qims-20-921 [doi]
AB  - BACKGROUND: Genetically encoded calcium indicators (GECIs), especially the 
      GCaMP-based green fluorescence GECIs have been widely used for in vivo detection 
      of neuronal activity in rodents by measuring intracellular neuronal Ca(2+) 
      changes. More recently, jRGECO1a, a red shifted GECI, has been reported to detect 
      neuronal Ca(2+) activation. This opens the possibility of using dual-color GECIs 
      for simultaneous interrogation of different cell populations. However, there has 
      been no report to compare the functional difference between these two GECIs for 
      in vivo imaging. Here, a comparative study is reported on neuronal responses to 
      sensory stimulation using GCaMP6f and jRGECO1a that were virally delivered into 
      the neurons in the somatosensory cortex of two different groups of animals, 
      respectively. METHODS: GCaMP6f and jRGECO1a GECI were virally delivered to 
      sensory cortex. After 3-4 weeks, the animals were imaged to capture the 
      spatiotemporal changes of neuronal Ca(2+) and the hemodynamic responses to 
      forepaw electrical stimulation (0.3 mA, 0.3 ms/pulse, 0.03 Hz). The 
      stimulation-evoked neuronal Ca(2+) transients expressed with GCaMP6f or jRGECO1a 
      were recorded during the baseline period and after an acute cocaine 
      administration (1 mg/kg, i.v.). RESULTS: Histology confirmed that the efficiency 
      of jRGECO1a and GCaMP6f expression into the cortical neurons was similar, i.e., 
      34%+/-3% and 32.7%+/-1.6%, respectively. Our imaging in vivo showed that the 
      hemodynamic responses to the stimulation were the same between jRGECO1a and 
      GCaMP6f expressed groups. Although the stimulation-evoked fluorescence change 
      (∆F/F) and the time-to-peak of the neuronal Ca(2+) transients were not 
      significantly different between these two indicators, the full-width-half-maximum 
      (FWHM) duration of the ∆F/F rise in the jRGECO1a-expressed group (0.16+/-0.02 s) 
      was ~50 ms or 46% longer than that of the GCaMP6f group (0.11+/-0.003 s), 
      indicating a longer recovery time in jRGECO1a than in GCaMP6f transients 
      (P<0.01). This is likely due to the longer off rate of jRGECO1a than that of 
      GCaMP6f. After cocaine, the time-to-peak of Ca(2+) transients was delayed and 
      their FWHM duration was prolonged for both expression groups, indicating that 
      these are cocaine's effects on neuronal Ca(2+) signaling and not artifacts due to 
      the property differences of the GCEIs. CONCLUSIONS: This study shows that both 
      jRGECO1a and GCaMP6f have sufficient sensitivity for tracking 
      single-stimulation-evoked Ca(2+) transients to detect neuronal activities from 
      the brain. Since these GECIs are emitted at the different wavelengths, it will be 
      possible to use them together to characterize the activity of different cell 
      types (e.g., neurons and astrocytes) to study brain activation and brain 
      functional changes in normal or diseased brains.
CI  - 2021 Quantitative Imaging in Medicine and Surgery. All rights reserved.
FAU - Park, Kicheon
AU  - Park K
AD  - Department of Biomedical Engineering, Stony Brook University, Stony Brook, NY, 
      USA.
FAU - Liyanage, Anuki C
AU  - Liyanage AC
AD  - Department of Biomedical Engineering, Stony Brook University, Stony Brook, NY, 
      USA.
FAU - Koretsky, Alan P
AU  - Koretsky AP
AD  - Laboratory of Functional and Molecular Imaging, National Institute of 
      Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, 
      USA.
FAU - Pan, Yingtian
AU  - Pan Y
AD  - Department of Biomedical Engineering, Stony Brook University, Stony Brook, NY, 
      USA.
FAU - Du, Congwu
AU  - Du C
AD  - Department of Biomedical Engineering, Stony Brook University, Stony Brook, NY, 
      USA.
LA  - eng
GR  - R01 DA029718/DA/NIDA NIH HHS/United States
GR  - R21 DA042597/DA/NIDA NIH HHS/United States
GR  - RF1 DA048808/DA/NIDA NIH HHS/United States
PT  - Journal Article
PL  - China
TA  - Quant Imaging Med Surg
JT  - Quantitative imaging in medicine and surgery
JID - 101577942
PMC - PMC7829166
OTO - NOTNLM
OT  - Ca2+ transient
OT  - Cocaine
OT  - GCaMP fluorescence imaging
OT  - jRGECO1a fluorescence imaging
COIS- Conflicts of Interest: All authors have completed the ICMJE uniform disclosure 
      form (available at http://dx.doi.org/10.21037/qims-20-921). The special issue 
      "Advanced Optical Imaging in Biomedicine" was commissioned by the editorial 
      office without any funding or sponsorship. The authors have no other conflicts of 
      interest to declare.
EDAT- 2021/03/04 06:00
MHDA- 2021/03/04 06:01
PMCR- 2021/03/01
CRDT- 2021/03/03 05:47
PHST- 2021/03/03 05:47 [entrez]
PHST- 2021/03/04 06:00 [pubmed]
PHST- 2021/03/04 06:01 [medline]
PHST- 2021/03/01 00:00 [pmc-release]
AID - qims-11-03-998 [pii]
AID - 10.21037/qims-20-921 [doi]
PST - ppublish
SO  - Quant Imaging Med Surg. 2021 Mar;11(3):998-1009. doi: 10.21037/qims-20-921.