PMID- 33654898
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220210
IS  - 2331-8325 (Electronic)
IS  - 2331-8325 (Linking)
VI  - 9
IP  - 20
DP  - 2019 Oct 20
TI  - Intracerebral Injection of Streptozotocin to Model Alzheimer Disease in Rats.
PG  - e3397
LID - 10.21769/BioProtoc.3397 [doi]
LID - e3397
AB  - Animal models have promoted meaningful contribution to science including 
      Alzheimer's disease (AD) research. Several animal models for AD have been used, 
      most of them related to genetic mutations observed in familial AD. However, 
      sporadic form of AD, also named late-onset is the most frequent form of the 
      disease, which is multifactorial, being influenced by genetic, environmental and 
      lifestyle factors. Here, we describe a protocol of an AD-like pathology of the 
      sporadic form using Wistar rats by a single bilateral intracerebroventricular 
      (icv) injection of streptozotocin (STZ, 2 mg/kg). Icv injection of STZ induces 
      brain resistance to insulin and other pathological alterations related to those 
      observed in AD, such as cognitive impairment and accumulation of phosphorylated 
      tau protein and beta-amyloid in the brain. Thus, icv injection of STZ is a useful 
      tool to investigate the pathological mechanisms and the metabolic alterations 
      involved in AD and to propose new therapeutic approaches and neuroprotective 
      drugs.
CI  - Copyright (c) 2019 The Authors; exclusive licensee Bio-protocol LLC.
FAU - Moreira-Silva, Daniel
AU  - Moreira-Silva D
AD  - Graduate Program in Neuroscience and Cognition, Federal University of ABC, Sao 
      Bernardo do Campo, Brazil.
FAU - Vizin, Robson C L
AU  - Vizin RCL
AD  - Graduate Program in Neuroscience and Cognition, Federal University of ABC, Sao 
      Bernardo do Campo, Brazil.
FAU - Martins, Talita M S
AU  - Martins TMS
AD  - Undergraduate Program in Neuroscience, Federal University of ABC, Sao Bernardo do 
      Campo, Brazil.
FAU - Ferreira, Tatiana L
AU  - Ferreira TL
AD  - Center for Mathematics, Computing and Cognition, Federal University of ABC, Sao 
      Bernardo do Campo, Brazil.
FAU - Almeida, Maria C
AU  - Almeida MC
AD  - Center for Natural and Human Sciences, Federal University of ABC, Sao Bernardo do 
      Campo, Brazil.
FAU - Carrettiero, Daniel C
AU  - Carrettiero DC
AD  - Center for Natural and Human Sciences, Federal University of ABC, Sao Bernardo do 
      Campo, Brazil.
LA  - eng
PT  - Journal Article
DEP - 20191020
PL  - United States
TA  - Bio Protoc
JT  - Bio-protocol
JID - 101635102
PMC - PMC7853929
OTO - NOTNLM
OT  - Cognition
OT  - Glucose
OT  - Insulin
OT  - Memory
OT  - Protocol
OT  - STZ
OT  - Tau
OT  - beta-amyloid
COIS- Competing interestsThe authors declare no financial and non-financial competing 
      interests.
EDAT- 2019/10/20 00:00
MHDA- 2019/10/20 00:01
PMCR- 2020/10/20
CRDT- 2021/03/03 05:48
PHST- 2019/06/25 00:00 [received]
PHST- 2019/09/10 00:00 [revised]
PHST- 2019/09/12 00:00 [accepted]
PHST- 2021/03/03 05:48 [entrez]
PHST- 2019/10/20 00:00 [pubmed]
PHST- 2019/10/20 00:01 [medline]
PHST- 2020/10/20 00:00 [pmc-release]
AID - e3397 [pii]
AID - 3397 [pii]
AID - 10.21769/BioProtoc.3397 [doi]
PST - epublish
SO  - Bio Protoc. 2019 Oct 20;9(20):e3397. doi: 10.21769/BioProtoc.3397. eCollection 
      2019 Oct 20.