PMID- 33658008
OWN - NLM
STAT- MEDLINE
DCOM- 20211004
LR  - 20240226
IS  - 1471-2261 (Electronic)
IS  - 1471-2261 (Linking)
VI  - 21
IP  - 1
DP  - 2021 Mar 3
TI  - microRNA-130a-5p suppresses myocardial ischemia reperfusion injury by 
      downregulating the HMGB2/NF-kappaB axis.
PG  - 121
LID - 10.1186/s12872-020-01742-4 [doi]
LID - 121
AB  - BACKGROUND: Myocardial ischemia reperfusion injury (MIRI) is defined as tissue 
      injury in the pathological process of progressive aggravation in ischemic 
      myocardium after the occurrence of acute coronary artery occlusion. Research has 
      documented the involvement of microRNAs (miRs) in MIRI. However, there is obscure 
      information about the role of miR-130a-5p in MIRI. Herein, this study aims to 
      investigate the effect of miR-130a-5p on MIRI. METHODS: MIRI mouse models were 
      established. Then, the cardiac function and hemodynamics were detected using 
      ultrasonography and multiconductive physiological recorder. Functional assays in 
      miR-130a-5p were adopted to test the degrees of oxidative stress, mitochondrial 
      functions, inflammation and apoptosis. Hematoxylin and eosin (HE) staining was 
      performed to validate the myocardial injury in mice. Reverse transcription 
      quantitative polymerase chain reaction (RT-qPCR) was employed to assess the 
      expression patterns of miR-130a-5p, high mobility group box (HMGB)2 and NF-kappaB. 
      Then, dual-luciferase reporter gene assay was performed to elucidate the 
      targeting relation between miR-130a-5p and HMGB2. RESULTS: Disrupted structural 
      arrangement in MIRI mouse models was evident from HE staining. RT-qPCR revealed 
      that overexpressed miR-130a-5p alleviated MIRI, MIRI-induced oxidative stress and 
      mitochondrial disorder in the mice. Next, the targeting relation between 
      miR-130a-5p and HMGB2 was ascertained. Overexpressed HMGB2 annulled the 
      protective effects of miR-130a-5p in MIRI mice. Additionally, miR-130a-5p targets 
      HMGB2 to downregulate the nuclear factor kappa-B (NF-kappaB) axis, mitigating the 
      inflammatory injury induced by MIRI. CONCLUSION: Our study demonstrated that 
      miR-130a-5p suppresses MIRI by down-regulating the HMGB2/NF-kappaB axis. This 
      investigation may provide novel insights for development of MIRI treatments.
FAU - Li, Yong
AU  - Li Y
AUID- ORCID: 0000-0003-0889-9439
AD  - Department of Cardiology, Harrision International Peace Hospital, No. 180 Renmin 
      East Road, Hengshui, 053000, Hebei, People's Republic of China. 
      drliyong0927@163.com.
FAU - Zhang, Hongbo
AU  - Zhang H
AD  - Department of Cardiology, Harrision International Peace Hospital, No. 180 Renmin 
      East Road, Hengshui, 053000, Hebei, People's Republic of China.
FAU - Li, Zhanhu
AU  - Li Z
AD  - Department of Cardiology, Harrision International Peace Hospital, No. 180 Renmin 
      East Road, Hengshui, 053000, Hebei, People's Republic of China.
FAU - Yan, Xiaoju
AU  - Yan X
AD  - Department of Cardiology, Harrision International Peace Hospital, No. 180 Renmin 
      East Road, Hengshui, 053000, Hebei, People's Republic of China.
FAU - Li, Yuan
AU  - Li Y
AD  - Department of Cardiology, Harrision International Peace Hospital, No. 180 Renmin 
      East Road, Hengshui, 053000, Hebei, People's Republic of China.
FAU - Liu, Shuai
AU  - Liu S
AD  - Department of Cardiology, Harrision International Peace Hospital, No. 180 Renmin 
      East Road, Hengshui, 053000, Hebei, People's Republic of China.
LA  - eng
PT  - Journal Article
DEP - 20210303
PL  - England
TA  - BMC Cardiovasc Disord
JT  - BMC cardiovascular disorders
JID - 100968539
RN  - 0 (HMGB2 Protein)
RN  - 0 (MIRN130 microRNA, mouse)
RN  - 0 (MicroRNAs)
RN  - 0 (NF-kappa B)
SB  - IM
MH  - Animals
MH  - Disease Models, Animal
MH  - Down-Regulation
MH  - HMGB2 Protein/genetics/*metabolism
MH  - Male
MH  - Mice, Inbred C57BL
MH  - MicroRNAs/genetics/*metabolism
MH  - Mitochondria, Heart/genetics/metabolism/pathology
MH  - Myocardial Reperfusion Injury/genetics/metabolism/pathology/*prevention & control
MH  - Myocardium/*metabolism/pathology
MH  - NF-kappa B/*metabolism
MH  - Oxidative Stress
MH  - Phosphorylation
MH  - Signal Transduction
MH  - Mice
PMC - PMC7931544
OTO - NOTNLM
OT  - HMGB2
OT  - Mitochondrial disorder
OT  - Myocardium ischemia reperfusion injury
OT  - NF-kappab pathway
OT  - Oxidative stress
OT  - microRNA-130a-5p
COIS- The authors declare that they have no competing interests.
EDAT- 2021/03/05 06:00
MHDA- 2021/10/05 06:00
PMCR- 2021/03/03
CRDT- 2021/03/04 05:34
PHST- 2019/12/30 00:00 [received]
PHST- 2020/10/13 00:00 [accepted]
PHST- 2021/03/04 05:34 [entrez]
PHST- 2021/03/05 06:00 [pubmed]
PHST- 2021/10/05 06:00 [medline]
PHST- 2021/03/03 00:00 [pmc-release]
AID - 10.1186/s12872-020-01742-4 [pii]
AID - 1742 [pii]
AID - 10.1186/s12872-020-01742-4 [doi]
PST - epublish
SO  - BMC Cardiovasc Disord. 2021 Mar 3;21(1):121. doi: 10.1186/s12872-020-01742-4.