PMID- 33658990
OWN - NLM
STAT- MEDLINE
DCOM- 20210616
LR  - 20210616
IS  - 1664-3224 (Electronic)
IS  - 1664-3224 (Linking)
VI  - 11
DP  - 2020
TI  - Fructo-Oligosaccharides Modify Human DC Maturation and Peanut-Induced Autologous 
      T-Cell Response of Allergic Patients In Vitro.
PG  - 600125
LID - 10.3389/fimmu.2020.600125 [doi]
LID - 600125
AB  - BACKGROUND: Dendritic cells (DCs) play an important role in antigen presentation, 
      and are an interesting target for immune-modulation in allergies. Short- and 
      long-chain fructo-oligosaccharides (scFOS/lcFOS, FF) have immunomodulatory 
      capacities, and may influence the outcome of DC antigen presentation. OBJECTIVE: 
      This study investigated the effect of FF during DC maturation and allergen 
      presentation using cells of peanut-allergic patients in an autologous DC-T cell 
      assay. METHODS: CD14(+) and CD4(+) T cells were isolated from peanut-allergic 
      patients. CD14(+) monocytes were differentiated into immature DCs (imDCs), and 
      matured (matDCs) in the presence or absence of crude peanut-extract (CPE) and/or 
      FF, and co-cultured in an autologous DC-T cell assay. T cell polarization, 
      proliferation and cytokine production were measured. RESULTS: Expression of 
      maturation surface molecule markers on matDCs was not affected by CPE and/or FF. 
      By contrast, the IL-10 secretion by matDCs increased compared to imDCs, upon 
      exposure to CPE and FF compared to CPE alone. Also the IP-10 secretion increased 
      in CPE/FF-matDCs compared to imDC. CPE-matDCs enhanced IL-13 release in the 
      DC-T-cell assay and Treg polarization in presence or absence of FF. CPE/FF-DCs 
      tended to increase the Treg/Th1 and Treg/Th2 ratios compared to matDCs. The 
      proliferation of both Treg and Th2 cells tended to increase when T cells were 
      co-cultured with CPE-matDCs compared to matDCs, which became significant when 
      CPE-matDCs were also exposed to FF and a same tendency was shown for Th1 
      proliferation. CONCLUSION: Only in the presence of FF, CPE-matDCs produced 
      increased regulatory and Th1-related mediators. CPE-matDCs modified T cell 
      polarization and proliferation, and additional exposure to FF tended to enhance 
      Treg/Th2 and Treg/Th1 ratios instructed by CPE/FF-matDCs. However this effect was 
      not strong enough to suppress CPE-matDCs induced IL-13 release by Th-cells. This 
      indicates the ability of FF to modify DC maturation in the presence of an 
      allergen supporting a more Treg/Th1 prone direction of the successive allergen 
      specific Th2 cell response.
CI  - Copyright (c) 2021 Hayen, Knulst, Garssen, Otten and Willemsen.
FAU - Hayen, Simone M
AU  - Hayen SM
AD  - Department of Dermatology/Allergology, University Medical Center Utrecht, Utrecht 
      University, Utrecht, Netherlands.
AD  - Laboratory of Translational Immunology, University Medical Center Utrecht, 
      Utrecht, Netherlands.
FAU - Knulst, Andre C
AU  - Knulst AC
AD  - Department of Dermatology/Allergology, University Medical Center Utrecht, Utrecht 
      University, Utrecht, Netherlands.
FAU - Garssen, Johan
AU  - Garssen J
AD  - Division of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Faculty 
      of Science, Utrecht University, Utrecht, Netherlands.
AD  - Department of Immunology, Nutricia Research B.V., Utrecht, Netherlands.
FAU - Otten, Henny G
AU  - Otten HG
AD  - Laboratory of Translational Immunology, University Medical Center Utrecht, 
      Utrecht, Netherlands.
FAU - Willemsen, Linette E M
AU  - Willemsen LEM
AD  - Division of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Faculty 
      of Science, Utrecht University, Utrecht, Netherlands.
LA  - eng
PT  - Journal Article
PT  - Randomized Controlled Trial
PT  - Research Support, Non-U.S. Gov't
DEP - 20210215
PL  - Switzerland
TA  - Front Immunol
JT  - Frontiers in immunology
JID - 101560960
RN  - 0 (Immunologic Factors)
RN  - 0 (Oligosaccharides)
RN  - 0 (Plant Extracts)
RN  - 0 (fructooligosaccharide)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Arachis/*chemistry
MH  - Cells, Cultured
MH  - Dendritic Cells/*immunology/pathology
MH  - Female
MH  - Humans
MH  - Immunologic Factors/immunology/*pharmacology
MH  - Male
MH  - Middle Aged
MH  - Oligosaccharides/immunology/*pharmacology
MH  - Peanut Hypersensitivity/*immunology/pathology
MH  - Plant Extracts/chemistry/*pharmacology
MH  - T-Lymphocytes, Helper-Inducer/*immunology/pathology
PMC - PMC7917053
OTO - NOTNLM
OT  - T cells
OT  - dendritic cells
OT  - immunomodulation
OT  - non-digestible oligosaccharides
OT  - peanut allergy
COIS- JG is employed at the Utrecht University and at Nutricia Research B.V. LW works 
      at the Pharmacology division of the Utrecht University within a strategic 
      alliance with Nutricia Research B.V. The remaining authors declare that the 
      research was conducted in the absence of any commercial or financial 
      relationships that could be construed as a potential conflict of interest.
EDAT- 2021/03/05 06:00
MHDA- 2021/06/17 06:00
PMCR- 2020/01/01
CRDT- 2021/03/04 05:54
PHST- 2020/08/28 00:00 [received]
PHST- 2020/12/24 00:00 [accepted]
PHST- 2021/03/04 05:54 [entrez]
PHST- 2021/03/05 06:00 [pubmed]
PHST- 2021/06/17 06:00 [medline]
PHST- 2020/01/01 00:00 [pmc-release]
AID - 10.3389/fimmu.2020.600125 [doi]
PST - epublish
SO  - Front Immunol. 2021 Feb 15;11:600125. doi: 10.3389/fimmu.2020.600125. eCollection 
      2020.