PMID- 33659325
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220210
IS  - 2331-8325 (Electronic)
IS  - 2331-8325 (Linking)
VI  - 10
IP  - 12
DP  - 2020 Jun 20
TI  - Assessments of HLA-I Specificities of Anti-HLA-I Monoclonal Antibodies Using 
      Solid Phase Bead Arrays.
PG  - e3655
LID - 10.21769/BioProtoc.3655 [doi]
LID - e3655
AB  - Human leukocyte antigen class I (HLA-I) molecules are a group of 
      structurally-related cell surface proteins with a high degree of variability 
      within the population. While only up to six variants are expressed in an 
      individual person, the whole population contains thousands of different variants. 
      The ability to distinguish specific variants is important in the clinic to 
      determine compatibility during organ and bone marrow transplantation and in the 
      laboratory to study the biological properties of individual variants. Solid phase 
      bead arrays contain purified, individually identifiable HLA-I molecules that can 
      be used to determine antibody specificity for individual HLA-I proteins. This 
      method is high-throughput, highly specific, and allows for simultaneous screening 
      of antibodies against multiple HLA-I allotypes. The beads are particularly useful 
      for screening patient sera for the presence of donor-specific antibodies against 
      individual HLA-I variants (which can arise during pregnancy, blood transfusion, 
      or organ transplantation). Alternate approaches, such as the use of individual 
      HLA-I-expressing cell lines, are more time consuming, and such cell lines are 
      difficult to procure and standardize. The HLA-I beads are also useful to study 
      HLA-I specificity and selectivity for other receptors and binding partners.
CI  - Copyright (c) The Authors; exclusive licensee Bio-protocol LLC.
FAU - Zaitouna, Anita J
AU  - Zaitouna AJ
AD  - Department of Microbiology and Immunology, Michigan Medicine, 1150 W. Medical 
      Center Drive, University of Michigan, Ann Arbor, MI 48109, USA.
FAU - Ramon, Daniel S
AU  - Ramon DS
AD  - Department of Laboratory Medicine and Pathology, Mayo Clinic, Arizona, USA.
FAU - Raghavan, Malini
AU  - Raghavan M
AD  - Department of Microbiology and Immunology, Michigan Medicine, 1150 W. Medical 
      Center Drive, University of Michigan, Ann Arbor, MI 48109, USA.
LA  - eng
GR  - R01 AI044115/AI/NIAID NIH HHS/United States
GR  - R21 AI126054/AI/NIAID NIH HHS/United States
GR  - T32 AI007413/AI/NIAID NIH HHS/United States
GR  - T32 AI007528/AI/NIAID NIH HHS/United States
PT  - Journal Article
DEP - 20200620
PL  - United States
TA  - Bio Protoc
JT  - Bio-protocol
JID - 101635102
PMC - PMC7842659
OTO - NOTNLM
OT  - Affinity
OT  - Bead array
OT  - Immunology
OT  - Luminex beads
OT  - Monoclonal antibodies
OT  - Specificity
COIS- Competing interestsThe authors do not have any competing interests.
EDAT- 2021/03/05 06:00
MHDA- 2021/03/05 06:01
PMCR- 2021/06/20
CRDT- 2021/03/04 05:55
PHST- 2020/01/29 00:00 [received]
PHST- 2020/04/14 00:00 [revised]
PHST- 2020/04/27 00:00 [accepted]
PHST- 2021/03/04 05:55 [entrez]
PHST- 2021/03/05 06:00 [pubmed]
PHST- 2021/03/05 06:01 [medline]
PHST- 2021/06/20 00:00 [pmc-release]
AID - e3655 [pii]
AID - 3655 [pii]
AID - 10.21769/BioProtoc.3655 [doi]
PST - epublish
SO  - Bio Protoc. 2020 Jun 20;10(12):e3655. doi: 10.21769/BioProtoc.3655. eCollection 
      2020 Jun 20.