PMID- 33659361
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220210
IS  - 2331-8325 (Electronic)
IS  - 2331-8325 (Linking)
VI  - 10
IP  - 14
DP  - 2020 Jul 20
TI  - Metabolomic and Lipidomic Analysis of Bone Marrow Derived Macrophages.
PG  - e3693
LID - 10.21769/BioProtoc.3693 [doi]
LID - e3693
AB  - Macrophages are highly plastic immune cells that are capable of adopting a wide 
      array of functional phenotypes in response to environmental stimuli. The changes 
      in macrophage function are often supported and regulated by changes in cellular 
      metabolism. Capturing a comprehensive picture of metabolism is vital for 
      understanding the role of metabolic rewiring in the immune response. Here we 
      present a method for systematically quantifying the abundance of metabolites and 
      lipids in primary murine bone marrow derived macrophages (BMDMs). This method 
      simultaneously extracts polar metabolites and lipids from BMDMs using a rapid 
      two-phase extraction procedure. The polar metabolite fraction and lipid fraction 
      are subsequently analyzed by separate liquid chromatography-mass spectrometry 
      (LC-MS) methods for optimized coverage and quantification. This allows for a 
      comprehensive characterization of cellular metabolism that can be used to 
      understand the impact of a variety of environmental stimuli on macrophage 
      metabolism and function.
CI  - Copyright (c) 2020 The Authors; exclusive licensee Bio-protocol LLC.
FAU - Seim, Gretchen L
AU  - Seim GL
AD  - Morgridge Institute for Research, Madison, WI, USA.
AD  - Department of Nutritional Sciences, University of Wisconsin-Madison, Madison, WI, 
      USA.
FAU - John, Steven V
AU  - John SV
AD  - Morgridge Institute for Research, Madison, WI, USA.
FAU - Fan, Jing
AU  - Fan J
AD  - Morgridge Institute for Research, Madison, WI, USA.
AD  - Department of Nutritional Sciences, University of Wisconsin-Madison, Madison, WI, 
      USA.
LA  - eng
PT  - Journal Article
DEP - 20200720
PL  - United States
TA  - Bio Protoc
JT  - Bio-protocol
JID - 101635102
PMC - PMC7842587
OTO - NOTNLM
OT  - LC-MS
OT  - Lipidomics
OT  - Macrophage
OT  - Metabolism
OT  - Metabolomics
COIS- Competing interestsThe authors declare no competing interests.
EDAT- 2021/03/05 06:00
MHDA- 2021/03/05 06:01
PMCR- 2021/07/20
CRDT- 2021/03/04 05:55
PHST- 2019/11/29 00:00 [received]
PHST- 2020/05/27 00:00 [revised]
PHST- 2020/05/10 00:00 [accepted]
PHST- 2021/03/04 05:55 [entrez]
PHST- 2021/03/05 06:00 [pubmed]
PHST- 2021/03/05 06:01 [medline]
PHST- 2021/07/20 00:00 [pmc-release]
AID - e3693 [pii]
AID - 3693 [pii]
AID - 10.21769/BioProtoc.3693 [doi]
PST - epublish
SO  - Bio Protoc. 2020 Jul 20;10(14):e3693. doi: 10.21769/BioProtoc.3693. eCollection 
      2020 Jul 20.