PMID- 33659439
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220210
IS  - 2331-8325 (Electronic)
IS  - 2331-8325 (Linking)
VI  - 10
IP  - 19
DP  - 2020 Oct 5
TI  - Simple Time-lapse Imaging for Quantifying the Hydrostatic Production of Oxygenic 
      Photogranules.
PG  - e3784
LID - 10.21769/BioProtoc.3784 [doi]
LID - e3784
AB  - Oxygenic photogranules (OPGs) are dense, three-dimensional aggregates containing 
      a syntrophic, light-driven microbial community. Their temporal and spatial 
      development interests microbial ecologists working at the bioprocess engineering 
      interface, as this knowledge can be used to optimize biotechnological 
      applications, such as wastewater treatment and biomass valorization. The method 
      presented here enables the high-throughput quantification of photogranulation. 
      OPGs are produced from a loose sludge-like microbial matrix in hydrostatic batch 
      cultures exposed to light. This matrix transforms into a consolidated, roughly 
      spherical aggregate over time. Photogranulation is quantified by time-lapse 
      imaging coupled to automated image analysis. This allows studying the development 
      of many OPGs simultaneously and in a fully automated way to systematically test 
      what factors drive photogranulation. The protocol can also be used to quantify 
      other types of (a)biotic aggregation.
CI  - Copyright (c) 2020 The Authors; exclusive licensee Bio-protocol LLC.
FAU - Joosten, Esmee D
AU  - Joosten ED
AD  - INRAE, Univ Montpellier, LBE, 102 Avenue des Etangs, 11100, Narbonne, France.
FAU - Hamelin, Jerome
AU  - Hamelin J
AD  - INRAE, Univ Montpellier, LBE, 102 Avenue des Etangs, 11100, Narbonne, France.
FAU - Milferstedt, Kim
AU  - Milferstedt K
AD  - INRAE, Univ Montpellier, LBE, 102 Avenue des Etangs, 11100, Narbonne, France.
LA  - eng
PT  - Journal Article
DEP - 20201005
PL  - United States
TA  - Bio Protoc
JT  - Bio-protocol
JID - 101635102
PMC - PMC7842665
OTO - NOTNLM
OT  - Aggregation
OT  - Biofilms
OT  - Dynamics
OT  - Microbial mats
OT  - Oxygenic photogranules
OT  - Photogranulation
OT  - Spatialization
OT  - Time-lapse imaging
COIS- Competing interestsThe authors declare not to have any (non-)financial competing 
      interests.
EDAT- 2021/03/05 06:00
MHDA- 2021/03/05 06:01
PMCR- 2021/10/05
CRDT- 2021/03/04 05:55
PHST- 2020/04/27 00:00 [received]
PHST- 2020/07/29 00:00 [revised]
PHST- 2020/08/21 00:00 [accepted]
PHST- 2021/03/04 05:55 [entrez]
PHST- 2021/03/05 06:00 [pubmed]
PHST- 2021/03/05 06:01 [medline]
PHST- 2021/10/05 00:00 [pmc-release]
AID - e3784 [pii]
AID - 3784 [pii]
AID - 10.21769/BioProtoc.3784 [doi]
PST - epublish
SO  - Bio Protoc. 2020 Oct 5;10(19):e3784. doi: 10.21769/BioProtoc.3784. eCollection 
      2020 Oct 5.