PMID- 33662415
OWN - NLM
STAT- MEDLINE
DCOM- 20210721
LR  - 20210721
IS  - 1879-0003 (Electronic)
IS  - 0141-8130 (Linking)
VI  - 178
DP  - 2021 May 1
TI  - Inhibition behavior of Sennoside A and Sennoside C on amyloid fibrillation of 
      human lysozyme and its possible mechanism.
PG  - 424-433
LID - S0141-8130(21)00512-2 [pii]
LID - 10.1016/j.ijbiomac.2021.02.213 [doi]
AB  - Amyloid proteins were recognized as the crucial cause of many senile diseases. In 
      this study, the inhibitory effects of Sennoside A (SA) and Sennoside C (SC) on 
      amyloid fibrillation were evaluated by the combination of biophysical approaches 
      and molecular docking tool using human lysozyme (HL) as amyloid-forming model. 
      The results of thioflavin-T (ThT), 8-anilino-1-naphthalenesulfonic acid (ANS) and 
      congo red (CR) assays indicated that both SA and SC could inhibit the amyloid 
      fibrillation of HL in a dose-dependent manner. The IC(50) value of SA and SC on 
      HL fibrillation was 200.09 muM and 186.20 muM, respectively. These findings were 
      further verified by transmission electron microscopy (TEM) and atomic force 
      microscopy (AFM), which showed that the addition of SA or SC could sharply reduce 
      the amyloid fibrillation of HL. Additionally, the interactions of HL with SA and 
      SC were investigated by steady-state fluorescence spectra and molecular docking 
      studies. The results suggested that both SA and SC could bind to the binding 
      pocket of HL and form a stable complex mainly via hydrogen bonds, van-der-Waals 
      forces and hydrophobic interactions. In conclusion, our experiments revealed that 
      both SA and SC can significantly inhibit amyloid fibrillation of HL.
CI  - Copyright (c) 2021. Published by Elsevier B.V.
FAU - Gao, Wen
AU  - Gao W
AD  - Department of Pharmacy, Liaocheng University, Liaocheng, Shandong 252000, China.
FAU - Jin, Li
AU  - Jin L
AD  - Institute of Biopharmaceutical Research, Liaocheng University, Liaocheng, 
      Shandong 252000, China.
FAU - Liu, Chunhong
AU  - Liu C
AD  - Institute of Biopharmaceutical Research, Liaocheng University, Liaocheng, 
      Shandong 252000, China.
FAU - Zhang, Ning
AU  - Zhang N
AD  - Institute of Biopharmaceutical Research, Liaocheng University, Liaocheng, 
      Shandong 252000, China. Electronic address: zhangning1111@126.com.
FAU - Zhang, Ruiyan
AU  - Zhang R
AD  - Institute of Biopharmaceutical Research, Liaocheng University, Liaocheng, 
      Shandong 252000, China. Electronic address: zry147896@163.com.
FAU - Bednarikova, Zuzana
AU  - Bednarikova Z
AD  - Department of Biophysics, Institute of Experimental Physics, Slovak Academy of 
      Sciences, Watsonova 47, 04001 Kosice, Slovakia.
FAU - Gazova, Zuzana
AU  - Gazova Z
AD  - Department of Biophysics, Institute of Experimental Physics, Slovak Academy of 
      Sciences, Watsonova 47, 04001 Kosice, Slovakia.
FAU - Bhunia, Anirban
AU  - Bhunia A
AD  - Department of Biophysics, Bose Institute, P-1/12 CIT Scheme VII (M), 700054 
      Kolkata, India.
FAU - Siebert, Hans-Christian
AU  - Siebert HC
AD  - RI-B-NT Research Institute of Bioinformatics and Nanotechnology, Franziusallee 
      177, 24148 Kiel, Germany.
FAU - Dong, Huijun
AU  - Dong H
AD  - Department of Pharmacy, Liaocheng University, Liaocheng, Shandong 252000, China. 
      Electronic address: donghuijun_747@163.com.
LA  - eng
PT  - Journal Article
DEP - 20210301
PL  - Netherlands
TA  - Int J Biol Macromol
JT  - International journal of biological macromolecules
JID - 7909578
RN  - 0 (Amyloid)
RN  - 0 (Protein Aggregates)
RN  - 0 (Sennosides)
RN  - 8013-11-4 (Senna Extract)
RN  - EC 3.2.1.17 (Muramidase)
RN  - EC 3.2.1.17 (lysozyme C, human)
RN  - UQ166P8802 (sennoside C)
SB  - IM
MH  - Amyloid/*chemistry
MH  - Humans
MH  - Muramidase/*chemistry
MH  - *Protein Aggregates
MH  - Senna Extract/*chemistry
MH  - Sennosides/*chemistry
OTO - NOTNLM
OT  - Amyloid fibrillation
OT  - Sennoside A
OT  - Sennoside C
EDAT- 2021/03/05 06:00
MHDA- 2021/07/22 06:00
CRDT- 2021/03/04 20:12
PHST- 2020/09/27 00:00 [received]
PHST- 2021/02/24 00:00 [revised]
PHST- 2021/02/27 00:00 [accepted]
PHST- 2021/03/05 06:00 [pubmed]
PHST- 2021/07/22 06:00 [medline]
PHST- 2021/03/04 20:12 [entrez]
AID - S0141-8130(21)00512-2 [pii]
AID - 10.1016/j.ijbiomac.2021.02.213 [doi]
PST - ppublish
SO  - Int J Biol Macromol. 2021 May 1;178:424-433. doi: 10.1016/j.ijbiomac.2021.02.213. 
      Epub 2021 Mar 1.