PMID- 33663157
OWN - NLM
STAT- MEDLINE
DCOM- 20210308
LR  - 20210308
IS  - 1002-0098 (Print)
IS  - 1002-0098 (Linking)
VI  - 56
IP  - 3
DP  - 2021 Mar 9
TI  - [Wnt3a promotes osteogenic differentiation of periodontal ligament stem cell and 
      regeneration of alveolar bone in experimental periodontitis].
PG  - 268-275
LID - 10.3760/cma.j.cn112144-20200611-00334 [doi]
AB  - Objective: To explore the effects of Wnt3a on the proliferation, migration and 
      osteogenic differentiation of periodontal ligament stem cell (PDLSC) and to 
      identify the role of Wnt3a in alveolar bone regeneration in mouse experimental 
      periodontitis. Methods: The experiments were conducted by stimulating PDLSC using 
      Wnt3a of 5 different concentrations (0, 20, 100, 200, 500 mug/L) respectively. 
      Cell proliferation was detected by cell-counting assay, cell migration was 
      evaluated by Transwell assay and the expressions of osteogenic related genes 
      collagen Ⅰ (Col-Ⅰ), runt-related transcription factor 2 (Runx2) were examined by 
      real-time quantitative PCR (RT-qPCR). Poly lactic-co-glycolic acid 
      (PLGA)-Wnt3a-hyaluronic acid (HA) hydrogel was injected locally into the gingival 
      sulcus of mice with experimental periodontitis. After 1, 2, 4, and 8 weeks of 
      hydrogel injection, samples of maxillary alveolar bone were obtained. Micro-CT, 
      HE staining and immunohistochemical staining of osteogenesis related markers, 
      such as alkaline phosphatase (ALP), Runx2, osteocalcin (OCN), were used to 
      evaluate alveolar bone regeneration. Results: After 10 d of culture, Wnt3a with 
      concentrations of 20-500 mug/L significantly promoted the proliferation (P<0.01) 
      and the migration (P<0.01) of PDLSC. After 21 d of culture, the expression levels 
      of Col-Ⅰ mRNA were 0.96+/-0.27, 1.90+/-0.47, 2.18+/-0.24, 2.32+/-0.15 and 1.99+/-0.43 in 5 
      concentration groups respectively, and the expression levels of Runx2 mRNA were 
      1.08+/-0.15, 3.19+/-0.17, 6.19+/-0.28, 9.19+/-0.41 and 5.55+/-0.06, respectively. Both 
      expressions had significant statistical differences compared with the negative 
      control group (P<0.05). At 1, 2, 4, and 8 weeks, the Wnt3a hydrogel group had 
      less distance [(497.3+/-18.2), (455.7+/-12.5), (401.0+/-8.5), (362.3+/-15.5) mum] from the 
      cemento-enamel junction to alveolar bone crest compared with the periodontitis 
      group [(710.3+/-10.2), (614.0+/-16.4), (564.3+/-12.5), (502.3+/-6.8) mum] (P<0.01) and 
      weaker periodontal inflammation. Immunohistochemical results showed that the 
      expression levels of bone-related proteins of ALP (0.72+/-0.01), Runx2 (0.77+/-0.03) 
      and OCN (0.72+/-0.07) in the Wnt3a hydrogel group were increased compared with the 
      periodontitis group (P<0.01). Conclusions: Wnt3a might promote the proliferation, 
      migration and osteogenic differentiation of PDLSC and the alveolar bone 
      regeneration.
FAU - Shen, S
AU  - Shen S
AD  - Department of Periodontology, School and Hospital of Stomatology, Cheeloo College 
      of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue 
      Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral 
      Tissue Regeneration, Jinan 250012, China.
FAU - Sun, S J
AU  - Sun SJ
AD  - Department of Prosthodontics, School and Hospital of Stomatology, Cheeloo College 
      of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue 
      Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral 
      Tissue Regeneration, Jinan 250012, China.
FAU - Ge, S H
AU  - Ge SH
AD  - Department of Periodontology, School and Hospital of Stomatology, Cheeloo College 
      of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue 
      Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral 
      Tissue Regeneration, Jinan 250012, China.
LA  - chi
GR  - 81873716/National Natural Science Foundation of China/
GR  - 2018WLJH79/Future Program for Young Scholars of Shandong University/
PT  - Journal Article
PL  - China
TA  - Zhonghua Kou Qiang Yi Xue Za Zhi
JT  - Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese 
      journal of stomatology
JID - 8711066
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Alkaline Phosphatase
MH  - Animals
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Mice
MH  - Osteogenesis
MH  - *Periodontal Ligament
MH  - *Periodontitis
MH  - Stem Cells
EDAT- 2021/03/06 06:00
MHDA- 2021/03/09 06:00
CRDT- 2021/03/05 01:51
PHST- 2021/03/05 01:51 [entrez]
PHST- 2021/03/06 06:00 [pubmed]
PHST- 2021/03/09 06:00 [medline]
AID - 10.3760/cma.j.cn112144-20200611-00334 [doi]
PST - ppublish
SO  - Zhonghua Kou Qiang Yi Xue Za Zhi. 2021 Mar 9;56(3):268-275. doi: 
      10.3760/cma.j.cn112144-20200611-00334.