PMID- 33667297
OWN - NLM
STAT- MEDLINE
DCOM- 20220111
LR  - 20240405
IS  - 1945-239X (Electronic)
IS  - 0021-9665 (Print)
IS  - 0021-9665 (Linking)
VI  - 60
IP  - 1
DP  - 2022 Jan 1
TI  - Development and Validation of an LC-MS/MS Method for AC1LPSZG and 
      Pharmacokinetics Application in Rats.
PG  - 26-34
LID - 10.1093/chromsci/bmab020 [doi]
AB  - Promising preliminary clinical data have stimulated research on the use of the 
      mammalian target of rapamycin (mTOR) inhibitors in lung cancer. AC1LPSZG is an 
      mTOR inhibitor that can significantly reduce the viability in lung adenosquamous 
      carcinoma cell line HTB-178 cells, showing potential benefits in effective 
      control of non-small cell lung carcinomas. In this study, a sensitive LC-MS/MS 
      analytical method for quantification of AC1LPSZG has been developed and optimized 
      to a running time of 3 min per sample. A linear dose-response for quantification 
      was observed over the range of 10-5000 ng/mL in rat plasma with required 
      precision and accuracy. High extraction recovery was achieved in the ranges of 
      86.87-102.51% at QC levels from rat plasma without significant matrix effect. 
      Stability profile of AC1LPSZG in rat plasma and in extract after protein 
      precipitation suggested that samples should be processed within 6 h after 
      collection and stored at -80  degrees C until analysis within 30 days. The method was 
      successfully applied to plasma pharmacokinetics (PK) study of AC1LPSZG in rat, 
      showing the plasma drug concentration followed a two-compartment model.
CI  - (c) The Author(s) 2021. Published by Oxford University Press. All rights reserved. 
      For Permissions, please email: journals.permissions@oup.com.
FAU - Chen, Yuan
AU  - Chen Y
AD  - Department of Pharmaceutical and Environmental Health Sciences, College of 
      Pharmacy and Health Sciences, Texas Southern University, Houston, TX, USA.
FAU - Gao, Xiuqing
AU  - Gao X
AD  - Department of Pharmaceutical and Environmental Health Sciences, College of 
      Pharmacy and Health Sciences, Texas Southern University, Houston, TX, USA.
FAU - Gupta, Ritu
AU  - Gupta R
AD  - Department of Pharmaceutical and Environmental Health Sciences, College of 
      Pharmacy and Health Sciences, Texas Southern University, Houston, TX, USA.
FAU - Ma, Jing
AU  - Ma J
AD  - Department of Pharmaceutical and Environmental Health Sciences, College of 
      Pharmacy and Health Sciences, Texas Southern University, Houston, TX, USA.
FAU - Dere, Ruhee
AU  - Dere R
AD  - Center for Precision Environmental Health, Baylor College of Medicine, Houston, 
      TX, USA.
FAU - Liang, Dong
AU  - Liang D
AD  - Department of Pharmaceutical and Environmental Health Sciences, College of 
      Pharmacy and Health Sciences, Texas Southern University, Houston, TX, USA.
FAU - Xie, Huan
AU  - Xie H
AUID- ORCID: 0000-0002-0591-5619
AD  - Department of Pharmaceutical and Environmental Health Sciences, College of 
      Pharmacy and Health Sciences, Texas Southern University, Houston, TX, USA.
LA  - eng
GR  - G12 MD007605/MD/NIMHD NIH HHS/United States
GR  - U54 MD007605/MD/NIMHD NIH HHS/United States
GR  - RCMI, G12MD007605/NH/NIH HHS/United States
PT  - Journal Article
PL  - United States
TA  - J Chromatogr Sci
JT  - Journal of chromatographic science
JID - 0173225
SB  - IM
MH  - Animals
MH  - Chromatography, Liquid
MH  - *Plasma
MH  - Rats
MH  - Reproducibility of Results
MH  - *Tandem Mass Spectrometry
PMC - PMC8742608
EDAT- 2021/03/06 06:00
MHDA- 2022/01/12 06:00
PMCR- 2022/02/27
CRDT- 2021/03/05 17:08
PHST- 2020/07/01 00:00 [received]
PHST- 2020/12/08 00:00 [revised]
PHST- 2021/01/31 00:00 [accepted]
PHST- 2021/03/06 06:00 [pubmed]
PHST- 2022/01/12 06:00 [medline]
PHST- 2021/03/05 17:08 [entrez]
PHST- 2022/02/27 00:00 [pmc-release]
AID - 6153993 [pii]
AID - bmab020 [pii]
AID - 10.1093/chromsci/bmab020 [doi]
PST - ppublish
SO  - J Chromatogr Sci. 2022 Jan 1;60(1):26-34. doi: 10.1093/chromsci/bmab020.