PMID- 33671834
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210310
IS  - 2306-7381 (Electronic)
IS  - 2306-7381 (Linking)
VI  - 8
IP  - 2
DP  - 2021 Feb 22
TI  - Swine Small Intestine Sealing Performed by Different Vessel Sealing Devices: 
      Ex-Vivo Test.
LID - 10.3390/vetsci8020034 [doi]
LID - 34
AB  - This study aimed to evaluate the sealing quality of swine small intestine using 
      different laparoscopic radiofrequency vessel sealing devices (two 5 mm: RFVS-1 
      and -2; one 10 mm: RFVS-3) and a harmonic scalpel (HS) compared to golden 
      standard closure technique. The study was divided into two arms. In study arm 1: 
      n = 50 swine intestinal loops (10 per group) were transected with each instrument 
      and the loops in which the devices provided complete sealing, at the gross 
      inspection, were tested for maximum burst pressure (BP) and histological 
      evaluation and compared to an automatic linear stapler. After the BP tests, the 
      devices that achieved significantly lower BP values were excluded from the second 
      arm. The RFVS-1 and -3 provided statistically significant results and were used 
      in study arm 2, to obtain full-thickness biopsies along the antimesenteric border 
      of the loop and were compared with hand-sewn intestinal closure (n = 30; 10 per 
      group). The biopsies were histologically evaluated for thermal injury and 
      diagnostic features, and intestinal loops tested for BP. RFVS-3 achieved 
      comparable results (69.78 +/- 4.23 mmHg, interquartile range (IQR) 5.8) to stapler 
      closing technique (71.09 +/- 4.22 mmHg, IQR 4.38; p > 0.05), while the RFVS-1 
      resulted in significantly (p < 0.05) lower BP (45.28 +/- 15.23 mmHg, IQR 24.95) but 
      over the physiological range, conversely to RFVS-2 (20.16 +/- 7.19 mmHg, IQR 12.02) 
      and HS (not measurable). RFVS-3 resulted not significantly different (p > 0.05) 
      (45.09 +/- 8.75 mmHg, IQR 10.48) than Suture (35.71 +/- 17.51 mmHg, IQR 23.77); 
      RFVS-1 resulted significantly lower values (23.96 +/- 10.63 mmHg, IQR 9.62; p < 
      0.05). All biopsies were judged diagnostic. Data confirmed that RFVS-1 and -3 
      devices provided suitable intestinal sealing, with BP pressures over the 
      physiological range. Conversely, the HS and RFVS-2 should not be considered for 
      intestinal sealing. RFVS devices could be employed to obtain small intestine 
      stump closure or full-thickness biopsies. However, further studies should be 
      performed in live animals to assess the role of the healing process.
FAU - Lacitignola, Luca
AU  - Lacitignola L
AUID- ORCID: 0000-0003-4963-8673
AD  - Dipartimento dell'Emergenze e Trapianti di Organi (D.E.T.O.), Sezione di Cliniche 
      Veterinarie e Produzioni Animali, Universita degli Studi di Bari "Aldo Moro", 
      70124 Bari, Italy.
FAU - Imperante, Annarita
AU  - Imperante A
AUID- ORCID: 0000-0001-8731-7219
AD  - Dottorato di Ricerca in "Trapianti di Tessuti ed Organi e Terapie Cellulari", 
      Dipartimento dell'Emergenza e Trapianti di Organi (D.E.T.O.), Universita degli 
      Studi di Bari "Aldo Moro", 70124 Bari, Italy.
FAU - Trisciuzzi, Rodrigo
AU  - Trisciuzzi R
AD  - Dottorato di Ricerca in "Trapianti di Tessuti ed Organi e Terapie Cellulari", 
      Dipartimento dell'Emergenza e Trapianti di Organi (D.E.T.O.), Universita degli 
      Studi di Bari "Aldo Moro", 70124 Bari, Italy.
FAU - Zizzo, Nicola
AU  - Zizzo N
AUID- ORCID: 0000-0002-1434-415X
AD  - Dipartimento di Medicina Veterinaria, Sez. di Anatomia Patologica, Universita 
      degli Studi di Bari "Aldo Moro", 70010 Bari, Italy.
FAU - Crovace, Alberto Maria
AU  - Crovace AM
AD  - Dipartimento di Scienze Mediche di Base, Neuroscienze e Organi di Senso, 
      Universita degli Studi di Bari "Aldo Moro", 70124 Bari, Italy.
FAU - Staffieri, Francesco
AU  - Staffieri F
AUID- ORCID: 0000-0002-2085-376X
AD  - Dipartimento dell'Emergenze e Trapianti di Organi (D.E.T.O.), Sezione di Cliniche 
      Veterinarie e Produzioni Animali, Universita degli Studi di Bari "Aldo Moro", 
      70124 Bari, Italy.
LA  - eng
PT  - Journal Article
DEP - 20210222
PL  - Switzerland
TA  - Vet Sci
JT  - Veterinary sciences
JID - 101680127
PMC - PMC7926574
OTO - NOTNLM
OT  - burst pressure
OT  - full-thickness biopsy
OT  - harmonic scalpel
OT  - leak pressure
OT  - radiofrequency vessel sealing device
OT  - small intestine
OT  - swine
OT  - vessel sealing device
COIS- The authors declare no conflict of interest.
EDAT- 2021/03/07 06:00
MHDA- 2021/03/07 06:01
PMCR- 2021/02/22
CRDT- 2021/03/06 01:11
PHST- 2021/02/08 00:00 [received]
PHST- 2021/02/19 00:00 [accepted]
PHST- 2021/03/06 01:11 [entrez]
PHST- 2021/03/07 06:00 [pubmed]
PHST- 2021/03/07 06:01 [medline]
PHST- 2021/02/22 00:00 [pmc-release]
AID - vetsci8020034 [pii]
AID - vetsci-08-00034 [pii]
AID - 10.3390/vetsci8020034 [doi]
PST - epublish
SO  - Vet Sci. 2021 Feb 22;8(2):34. doi: 10.3390/vetsci8020034.