PMID- 33671870
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210316
IS  - 2075-4450 (Print)
IS  - 2075-4450 (Electronic)
IS  - 2075-4450 (Linking)
VI  - 12
IP  - 2
DP  - 2021 Feb 15
TI  - Physical Mapping of the Anopheles (Nyssorhynchus) darlingi Genomic Scaffolds.
LID - 10.3390/insects12020164 [doi]
LID - 164
AB  - The genome assembly of Anopheles darlingi consists of 2221 scaffolds (N50 = 
      115,072 bp) and has a size spanning 136.94 Mbp. This assembly represents one of 
      the smallest genomes among Anopheles species. Anopheles darlingi genomic DNA 
      fragments of ~37 Kb were cloned, end-sequenced, and used as probes for 
      fluorescence in situ hybridization (FISH) with salivary gland polytene 
      chromosomes. In total, we mapped nine DNA probes to scaffolds and autosomal arms. 
      Comparative analysis of the An. darlingi scaffolds with homologous sequences of 
      the Anopheles albimanus and Anopheles gambiae genomes identified chromosomal 
      rearrangements among these species. Our results confirmed that physical mapping 
      is a useful tool for anchoring genome assemblies to mosquito chromosomes.
FAU - Rafael, Miriam Silva
AU  - Rafael MS
AUID- ORCID: 0000-0003-4726-855X
AD  - Coordenacao de Sociedade Ambiente e Saude, Laboratorio de Vetores de Malaria e 
      Dengue, Instituto Nacional de Pesquisas da Amazonia, Av. Andre Araujo, 2936, 
      Manaus, AM 69060-001, Brazil.
AD  - Programa de Pos-Graduacao em Genetica, Conservacao e Biologia Evolutiv, Instituto 
      Nacional de Pesquisas da Amazonia, Manaus, AM 69060-001, Brazil.
FAU - Bridi, Leticia Cegatti
AU  - Bridi LC
AUID- ORCID: 0000-0001-8470-4205
AD  - Programa de Pos-Graduacao em Genetica, Conservacao e Biologia Evolutiv, Instituto 
      Nacional de Pesquisas da Amazonia, Manaus, AM 69060-001, Brazil.
FAU - Sharakhov, Igor V
AU  - Sharakhov IV
AUID- ORCID: 0000-0003-0752-3747
AD  - Department of Entomology and Fralin Life Science Institute, Virginia Polytechnic 
      Institute and State University, Blacksburg, VA 24061, USA.
AD  - Laboratory of Evolutionary Genomics of Insects, the Federal Research Center 
      Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of 
      Sciences, 630090 Novosibirsk, Russia.
AD  - Department of Genetics and Cell Biology, Tomsk State University, 634050 Tomsk, 
      Russia.
FAU - Marinotti, Osvaldo
AU  - Marinotti O
AUID- ORCID: 0000-0002-7173-7160
AD  - MTEKPrime, Aliso Viejo, CA 92656, USA.
FAU - Sharakhova, Maria V
AU  - Sharakhova MV
AUID- ORCID: 0000-0002-5790-3548
AD  - Department of Entomology and Fralin Life Science Institute, Virginia Polytechnic 
      Institute and State University, Blacksburg, VA 24061, USA.
AD  - Laboratory of Evolutionary Genomics of Insects, the Federal Research Center 
      Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of 
      Sciences, 630090 Novosibirsk, Russia.
FAU - Timoshevskiy, Vladimir
AU  - Timoshevskiy V
AD  - Department of Entomology and Fralin Life Science Institute, Virginia Polytechnic 
      Institute and State University, Blacksburg, VA 24061, USA.
AD  - Department of Biology, University of Kentucky, Lexington, KY 40506, USA.
FAU - Guimaraes-Marques, Giselle Moura
AU  - Guimaraes-Marques GM
AUID- ORCID: 0000-0002-9944-3586
AD  - Programa de Pos-Graduacao em Genetica, Conservacao e Biologia Evolutiv, Instituto 
      Nacional de Pesquisas da Amazonia, Manaus, AM 69060-001, Brazil.
FAU - Santos, Valeria Silva
AU  - Santos VS
AD  - Programa de Pos-Graduacao em Genetica, Conservacao e Biologia Evolutiv, Instituto 
      Nacional de Pesquisas da Amazonia, Manaus, AM 69060-001, Brazil.
FAU - da Silva, Carlos Gustavo Nunes
AU  - da Silva CGN
AD  - Programa de Pos-Graduacao em Biotecnologia, Universidade Federal do Amazonas, Av. 
      Rodrigo Otavio, 6.200. Coroado l, Manaus, AM 69080-900, Brazil.
FAU - Astolfi-Filho, Spartaco
AU  - Astolfi-Filho S
AD  - Laboratorio de Tecnologias de DNA, Divisao de Biotecnologia, Centro de Apoio 
      Multidisciplinar, Universi dade Federal do Amazonas, Av. Rodrigo Otavio, 6.200. 
      Coroado l, Manaus, AM 69080-900, Brazil.
FAU - Tadei, Wanderli Pedro
AU  - Tadei WP
AUID- ORCID: 0000-0002-0612-3285
AD  - Coordenacao de Sociedade Ambiente e Saude, Laboratorio de Vetores de Malaria e 
      Dengue, Instituto Nacional de Pesquisas da Amazonia, Av. Andre Araujo, 2936, 
      Manaus, AM 69060-001, Brazil.
AD  - Programa de Pos-Graduacao em Genetica, Conservacao e Biologia Evolutiv, Instituto 
      Nacional de Pesquisas da Amazonia, Manaus, AM 69060-001, Brazil.
LA  - eng
PT  - Journal Article
DEP - 20210215
PL  - Switzerland
TA  - Insects
JT  - Insects
JID - 101574235
PMC - PMC7918962
OTO - NOTNLM
OT  - genomics and cytogenetics
OT  - in situ hybridization
OT  - polytene chromosome
OT  - synteny
COIS- The authors declare no conflict of interest.
EDAT- 2021/03/07 06:00
MHDA- 2021/03/07 06:01
PMCR- 2021/02/15
CRDT- 2021/03/06 01:11
PHST- 2020/12/16 00:00 [received]
PHST- 2021/02/09 00:00 [revised]
PHST- 2021/02/11 00:00 [accepted]
PHST- 2021/03/06 01:11 [entrez]
PHST- 2021/03/07 06:00 [pubmed]
PHST- 2021/03/07 06:01 [medline]
PHST- 2021/02/15 00:00 [pmc-release]
AID - insects12020164 [pii]
AID - insects-12-00164 [pii]
AID - 10.3390/insects12020164 [doi]
PST - epublish
SO  - Insects. 2021 Feb 15;12(2):164. doi: 10.3390/insects12020164.