PMID- 33672928
OWN - NLM
STAT- MEDLINE
DCOM- 20210419
LR  - 20211204
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 22
IP  - 4
DP  - 2021 Feb 14
TI  - Low-Temperature Argon Plasma Regulates Skin Moisturizing and 
      Melanogenesis-Regulating Markers through Yes-Associated Protein.
LID - 10.3390/ijms22041895 [doi]
LID - 1895
AB  - Extensive water loss and melanin hyperproduction can cause various skin 
      disorders. Low-temperature argon plasma (LTAP) has shown the possibility of being 
      used for the treatment of various skin diseases, such as atopic dermatitis and 
      skin cancer. However, the role of LTAP in regulating skin moisturizing and 
      melanogenesis has not been investigated. In this study, we aimed to determine the 
      effect of LTAP on yes-associated protein (YAP), a major transcriptional 
      coactivator in the Hippo signaling pathway that is involved in skin moisturizing 
      and melanogenesis-regulating markers. In normal human epidermal keratinocytes 
      (NHEKs), the human epidermal keratinocyte line HaCaT, and human dermal 
      fibroblasts (HDFs), we found that LTAP exhibited increased expression levels of 
      YAP protein. In addition, the expression levels of filaggrin (FLG), which is 
      involved in natural moisturizing factors (NMFs), and hyaluronic acid synthase 
      (HAS), transglutaminase (TGM), and involucrin (IVL), which regulate skin barrier 
      and moisturizing, were also increased after exposure to LTAP. Furthermore, 
      collagen type I alpha 1 and type III alpha 1 (COL1A1, COL3A1) were increased 
      after LTAP exposure, but the expression level of matrix metalloproteinase-3 
      (MMP-3) was reduced. Moreover, LTAP was found to suppress alpha-melanocyte 
      stimulating hormone (alpha-MSH)-induced melanogenesis in murine melanoma B16F10 cells 
      and normal human melanocytes (NHEMs). LTAP regulates melanogenesis of the 
      melanocytes through decreased YAP pathway activation in a melanocortin 1 receptor 
      (MC1R)-dependent manner. Taken together, our data show that LTAP regulates skin 
      moisturizing and melanogenesis through modulation of the YAP pathway, and the 
      effect of LTAP on the expression level of YAP varies from cell to cell. Thus, 
      LTAP might be developed as a treatment method to improve the skin barrier, 
      moisture content, and wrinkle formation, and to reduce melanin generation.
FAU - Kim, Hae-Young
AU  - Kim HY
AD  - Department of Dermato-Immunology, College of Medicine, Catholic University of 
      Korea, Seoul 06591, Korea.
FAU - Agrahari, Gaurav
AU  - Agrahari G
AUID- ORCID: 0000-0003-0407-7083
AD  - Department of Dermato-Immunology, College of Medicine, Catholic University of 
      Korea, Seoul 06591, Korea.
FAU - Lee, Min Jung
AU  - Lee MJ
AD  - Department of Dermato-Immunology, College of Medicine, Catholic University of 
      Korea, Seoul 06591, Korea.
FAU - Tak, Lee-Jung
AU  - Tak LJ
AD  - Department of Dermato-Immunology, College of Medicine, Catholic University of 
      Korea, Seoul 06591, Korea.
FAU - Ham, Won-Kook
AU  - Ham WK
AD  - Department of Dermato-Immunology, College of Medicine, Catholic University of 
      Korea, Seoul 06591, Korea.
FAU - Kim, Tae-Yoon
AU  - Kim TY
AD  - Department of Dermato-Immunology, College of Medicine, Catholic University of 
      Korea, Seoul 06591, Korea.
LA  - eng
GR  - 2016M3A9B6903020/National Research Foundation of Korea/
PT  - Journal Article
DEP - 20210214
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (COL1A1 protein, human)
RN  - 0 (Collagen Type I, alpha 1 Chain)
RN  - 0 (FLG protein, human)
RN  - 0 (Filaggrin Proteins)
RN  - 0 (Melanins)
RN  - 0 (Plasma Gases)
RN  - 0 (Receptor, Melanocortin, Type 1)
RN  - 0 (Transcription Factors)
RN  - 0 (YAP-Signaling Proteins)
RN  - 0 (YAP1 protein, human)
RN  - 581-05-5 (alpha-MSH)
RN  - 67XQY1V3KH (Argon)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing/*metabolism
MH  - Animals
MH  - Argon/*pharmacology
MH  - Cell Line
MH  - Cell Line, Tumor
MH  - Cells, Cultured
MH  - Collagen Type I, alpha 1 Chain
MH  - Filaggrin Proteins
MH  - Humans
MH  - Keratinocytes/cytology/drug effects/metabolism
MH  - Melanins/*metabolism
MH  - Melanocytes/cytology/drug effects/metabolism
MH  - Mice
MH  - Plasma Gases/*pharmacology
MH  - Receptor, Melanocortin, Type 1/metabolism
MH  - Skin/*drug effects/metabolism
MH  - Skin Aging/drug effects
MH  - Temperature
MH  - Transcription Factors/*metabolism
MH  - YAP-Signaling Proteins
MH  - alpha-MSH/metabolism
PMC - PMC7918577
OTO - NOTNLM
OT  - low-temperature argon plasma
OT  - melanogenesis
OT  - melanoma
OT  - skin barrier
OT  - skin moisturizing
OT  - yes-associated protein
COIS- The authors have no conflict of interest to declare.
EDAT- 2021/03/07 06:00
MHDA- 2021/04/20 06:00
PMCR- 2021/02/14
CRDT- 2021/03/06 01:14
PHST- 2020/12/18 00:00 [received]
PHST- 2021/02/01 00:00 [revised]
PHST- 2021/02/03 00:00 [accepted]
PHST- 2021/03/06 01:14 [entrez]
PHST- 2021/03/07 06:00 [pubmed]
PHST- 2021/04/20 06:00 [medline]
PHST- 2021/02/14 00:00 [pmc-release]
AID - ijms22041895 [pii]
AID - ijms-22-01895 [pii]
AID - 10.3390/ijms22041895 [doi]
PST - epublish
SO  - Int J Mol Sci. 2021 Feb 14;22(4):1895. doi: 10.3390/ijms22041895.