PMID- 33673763
OWN - NLM
STAT- MEDLINE
DCOM- 20220211
LR  - 20220211
IS  - 1530-8022 (Electronic)
IS  - 0885-3282 (Linking)
VI  - 36
IP  - 3
DP  - 2021 Sep
TI  - Experimental study on repair of large segmental bone defects of goat femur by 
      nano calcium-deficient hydroxyapatite-multi (amino acid) copolymer membrane 
      tubes.
PG  - 492-502
LID - 10.1177/08853282211000298 [doi]
AB  - OBJECTIVE: The purpose of this study was to observe feasibility of nano 
      calcium-deficient hydroxyapatite-multi (amino acid) copolymer (n-CDHA-MAC) 
      membrane tubes in repairing goat femurs' large defects. METHODS: Twelve goats 
      were divided into two groups, whose femurs were created 30 mm segmental bone 
      defects and then implants were performed. In experimental group, the bone defect 
      of right femur was reconstructed by n-CDHA-MAC membrane tube, while left side was 
      reconstructed by allogenic bone tube in control group. Every three goats were 
      sacrificed at 4, 8, 16, 24 weeks after operation respectively. General 
      observation, X-ray analysis, histology, Scanning electron microscope (SEM) 
      examination and protein level comparison of BMP-2 were conducted to evaluate the 
      effects of repairing segmental bone defects. RESULTS: All goats recovered well 
      from anesthesia and surgical interventions. The radiographic evaluations showed 
      that periosteal reaction outside of the membrane tubes and allogenic bone tubes 
      were observed 4 weeks after surgery. At 16 weeks, callus was continuously 
      increased in experimental group, which was more obvious than control group. At 
      24 weeks, callus outside of the membrane tubes connected together. Histologic 
      evaluation showed fibro-cartilage callus was evolved into bony callus in 
      experimental group, which was more obvious than control group at 8 and 16 weeks. 
      The protein expression level of BMP-2 increased at 4, 8 weeks and peaked at 
      16 weeks in experimental groups. There were statistical differences at 8 and 
      16 weeks (P < 0.05). At each time point in 8, 16, 24 weeks after surgery, the 
      bending stiffness, torsional stiffness and compressive strength of the two groups 
      were similar, and there was no significant difference (P > 0.05). CONCLUSIONS: 
      This novel surface degradation n-CDHA-MAC membrane tube has good ability to 
      maintain enough membrane space, which can provide long-term and stable 
      biomechanical support for large bone defects and integrate well with the 
      surrounding bone.
FAU - Xiong, Yan
AU  - Xiong Y
AUID- ORCID: 0000-0001-6130-5842
AD  - Department of Orthopedics, West China Hospital, Sichuan University, Sichuan, 
      China.
FAU - Duan, Hong
AU  - Duan H
AD  - Department of Orthopedics, West China Hospital, Sichuan University, Sichuan, 
      China.
FAU - Zhang, Bin
AU  - Zhang B
AD  - Department of Orthopedics, West China Hospital, Sichuan University, Sichuan, 
      China.
FAU - Ren, Cheng
AU  - Ren C
AD  - Department of Orthopedics, West China Hospital, Sichuan University, Sichuan, 
      China.
FAU - Yu, Zeping
AU  - Yu Z
AD  - Department of Orthopedics, West China Hospital, Sichuan University, Sichuan, 
      China.
FAU - Yan, Yonggang
AU  - Yan Y
AD  - College of Physical Science and Technology, Sichuan University, Sichuan, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210305
PL  - England
TA  - J Biomater Appl
JT  - Journal of biomaterials applications
JID - 8813912
RN  - 0 (Amino Acids)
RN  - 0 (Bone Substitutes)
RN  - 0 (Membranes, Artificial)
RN  - 91D9GV0Z28 (Durapatite)
SB  - IM
MH  - Amino Acids/*therapeutic use
MH  - Animals
MH  - Bone Substitutes/*therapeutic use
MH  - Durapatite/*therapeutic use
MH  - Female
MH  - Femur/*injuries
MH  - Goats
MH  - Male
MH  - Membranes, Artificial
OTO - NOTNLM
OT  - Nano calcium-deficient hydroxyapatite-multi (amino acid) copolymer
OT  - allogenic bone
OT  - animal experiments
OT  - guided bone regeneration
OT  - large segmental bone defects
OT  - osseointegration
EDAT- 2021/03/07 06:00
MHDA- 2022/02/12 06:00
CRDT- 2021/03/06 05:27
PHST- 2021/03/07 06:00 [pubmed]
PHST- 2022/02/12 06:00 [medline]
PHST- 2021/03/06 05:27 [entrez]
AID - 10.1177/08853282211000298 [doi]
PST - ppublish
SO  - J Biomater Appl. 2021 Sep;36(3):492-502. doi: 10.1177/08853282211000298. Epub 
      2021 Mar 5.