PMID- 3367538
OWN - NLM
STAT- MEDLINE
DCOM- 19880621
LR  - 20191029
IS  - 0021-5120 (Print)
IS  - 0021-5120 (Linking)
VI  - 27
IP  - 1
DP  - 1988 Feb
TI  - Activities, electrophoretic profiles and immunolocalization of superoxide 
      dismutase in human liver specimens.
PG  - 34-41
AB  - In order to elucidate active oxygen in liver diseases, activities, 
      electrophoretic profiles and immunolocalization of superoxide dismutase (SOD) in 
      human liver specimens were investigated. Activities and electrophoresis were 
      studied using liver homogenates in 41 cases and immunolocalization of Cu, Zn SOD 
      was observed in 87 cases. Total SOD activity in acute viral hepatitis (AVH) and 
      fatty liver (FL) groups was significantly lower than that in non-specific 
      reactive hepatitis (NSRH) group. Cu, Zn SOD activity in AVH, FL and chronic 
      active hepatitis (CAH) groups was also significantly lower than that in NSRH 
      group. However, no difference of Mn SOD activity, was found between NSRH group 
      and others. Decreased activity of superoxide dismutase in liver tissues suggests 
      the release of this enzyme from the injured hepatocytes. In electrophoretic 
      patterns of superoxide dismutase, 3 bands of Cu, Zn SOD isozymes and 8 to 10 
      bands of Mn SOD isozymes were recognized. Immunocytochemical investigation 
      revealed the localization of Cu, Zn SOD in the cytoplasm of hepatocytes. Two 
      different distribution of Cu, Zn SOD was observed in the lobules: a diffuse 
      localization pattern and a focal one. The latter was found in the cases of liver 
      diseases with severe parenchymal lesion. These findings suggest that superoxide 
      radical anion and its scavenger, superoxide dismutase, may play an important role 
      in the pathogenesis of liver cell necrosis.
FAU - Yasuyama, T
AU  - Yasuyama T
AD  - Third Department of Internal Medicine, Faculty of Medicine, Toyama Medical and 
      Pharmaceutical University, Japan.
FAU - Inoue, K
AU  - Inoue K
FAU - Kojima, T
AU  - Kojima T
FAU - Sasaki, H
AU  - Sasaki H
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Jpn J Med
JT  - Japanese journal of medicine
JID - 0247713
RN  - 0 (Free Radicals)
RN  - 0 (Isoenzymes)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Chemical and Drug Induced Liver Injury
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Free Radicals
MH  - Humans
MH  - Immunohistochemistry
MH  - Isoelectric Focusing
MH  - Isoenzymes/genetics
MH  - Liver/*enzymology
MH  - Liver Diseases/*enzymology
MH  - Oxygen/metabolism
MH  - Superoxide Dismutase/*analysis
EDAT- 1988/02/01 00:00
MHDA- 1988/02/01 00:01
CRDT- 1988/02/01 00:00
PHST- 1988/02/01 00:00 [pubmed]
PHST- 1988/02/01 00:01 [medline]
PHST- 1988/02/01 00:00 [entrez]
AID - 10.2169/internalmedicine1962.27.34 [doi]
PST - ppublish
SO  - Jpn J Med. 1988 Feb;27(1):34-41. doi: 10.2169/internalmedicine1962.27.34.