PMID- 33676089
OWN - NLM
STAT- MEDLINE
DCOM- 20210917
LR  - 20210917
IS  - 1873-2534 (Electronic)
IS  - 0165-2427 (Linking)
VI  - 234
DP  - 2021 Apr
TI  - Heterogeneous populations from in vitro cultures of antigen presenting cells in 
      pigs.
PG  - 110215
LID - S0165-2427(21)00033-7 [pii]
LID - 10.1016/j.vetimm.2021.110215 [doi]
AB  - Dendritic cells (DCs) are the most potent antigen presenting cells (APCs). 
      Because of the difficulty in obtaining these cells directly from tissues, 
      different sources of DCs are frequently used for in vitro experimentation and 
      many of their biological and functional characteristics were studied using these 
      systems. Until recently, it was assumed that specific culture conditions 
      polarized the differentiation of either DCs or macrophages (Macs); however, it 
      was shown that some DC culture systems in other species generate heterogeneous 
      cell populations that can be identified according to their CD11c and MHC class II 
      (MHC-II) expression. Following this approach, porcine DCs were directly isolated 
      from peripheral blood or differentiated in vitro by culturing bone marrow (BM) 
      progenitor cells or blood monocytes treated with growth factors. Mostly 
      homogeneous monocyte-derived DCs (MoDCs) were obtained with similar phenotype and 
      phagocytic characteristics to that of blood DCs. On the contrary, BM-derived DC 
      (BMDC) cultures generated two distinct heterogeneous populations identified as 
      MHC-II(+) and MHC-II(++) cells. BMDCs MHC-II(+) had similar phenotypic and 
      phagocytic characteristics to those of MoDCs and blood DCs. However, BMDCs 
      MHC-II(++) population expressed a higher amount of surface markers and 
      transcribed genes associated with Macs-lineage exhibiting a higher phagocytic 
      capacity than all the other cells. Noteworthy, every cell system expressed 
      different genetic signatures. These results will help interpreting and 
      re-interpreting data obtained using in vitro systems.
CI  - Copyright (c) 2021. Published by Elsevier B.V.
FAU - Pujol, Myriam
AU  - Pujol M
AD  - Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada; The 
      Pirbright Institute, Pirbright, Woking, GU24 0NF, UK.
FAU - Guzman, Efrain
AU  - Guzman E
AD  - The Pirbright Institute, Pirbright, Woking, GU24 0NF, UK.
FAU - Montaner-Tarbes, Sergio
AU  - Montaner-Tarbes S
AD  - The Pirbright Institute, Pirbright, Woking, GU24 0NF, UK; Department of Animal 
      Production, Universitat de Lleida, 25198, Lleida, Spain; Innovex Therapeutics SL. 
      Badalona, Spain.
FAU - Montoya, Maria
AU  - Montoya M
AD  - The Pirbright Institute, Pirbright, Woking, GU24 0NF, UK; Centro de 
      Investigaciones Biologicas (CIB-CSIC), Ramiro de Maeztu 9, Madrid, Spain. 
      Electronic address: maria.montoya@cib.csic.es.
LA  - eng
GR  - BBS/E/I/00007039/BB_/Biotechnology and Biological Sciences Research 
      Council/United Kingdom
PT  - Journal Article
DEP - 20210225
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
RN  - 0 (Histocompatibility Antigens Class II)
SB  - IM
MH  - Age Factors
MH  - Animals
MH  - Antigen Presentation/*immunology
MH  - Antigen-Presenting Cells/classification/*immunology
MH  - Bone Marrow Cells/classification/*immunology
MH  - Cell Differentiation/*immunology
MH  - Cells, Cultured
MH  - Dendritic Cells/classification/immunology/physiology
MH  - Female
MH  - Flow Cytometry
MH  - Histocompatibility Antigens Class II/immunology
MH  - Lymphocyte Activation
MH  - Macrophages/immunology/physiology
MH  - Male
MH  - Monocytes/immunology
MH  - Swine
OTO - NOTNLM
OT  - Dendritic cells
OT  - Macrophages
OT  - Monocytes
OT  - Porcine in vitro-derived cells
EDAT- 2021/03/07 06:00
MHDA- 2021/09/18 06:00
CRDT- 2021/03/06 20:12
PHST- 2020/04/05 00:00 [received]
PHST- 2020/08/02 00:00 [revised]
PHST- 2021/02/10 00:00 [accepted]
PHST- 2021/03/07 06:00 [pubmed]
PHST- 2021/09/18 06:00 [medline]
PHST- 2021/03/06 20:12 [entrez]
AID - S0165-2427(21)00033-7 [pii]
AID - 10.1016/j.vetimm.2021.110215 [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 2021 Apr;234:110215. doi: 10.1016/j.vetimm.2021.110215. 
      Epub 2021 Feb 25.