PMID- 33679689
OWN - NLM
STAT- MEDLINE
DCOM- 20210617
LR  - 20210617
IS  - 1664-3224 (Electronic)
IS  - 1664-3224 (Linking)
VI  - 11
DP  - 2020
TI  - Microarray-Based Allergy Diagnosis: Quo Vadis?
PG  - 594978
LID - 10.3389/fimmu.2020.594978 [doi]
LID - 594978
AB  - More than 30% of the world population suffers from allergy. Allergic individuals 
      are characterized by the production of immunoglobulin E (IgE) antibodies against 
      innocuous environmental allergens. Upon allergen recognition IgE mediates 
      allergen-specific immediate and late-phase allergic inflammation in different 
      organs. The identification of the disease-causing allergens by demonstrating the 
      presence of allergen-specific IgE is the key to precision medicine in allergy 
      because it allows tailoring different forms of prevention and treatment according 
      to the sensitization profiles of individual allergic patients. More than 30 years 
      ago molecular cloning started to accelerate the identification of the 
      disease-causing allergen molecules and enabled their production as recombinant 
      molecules. Based on recombinant allergen molecules, molecular allergy diagnosis 
      was introduced into clinical practice and allowed dissecting the molecular 
      sensitization profiles of allergic patients. In 2002 it was demonstrated that 
      microarray technology allows assembling large numbers of allergen molecules on 
      chips for the rapid serological testing of IgE sensitizations with small volumes 
      of serum. Since then microarrayed allergens have revolutionized research and 
      diagnosis in allergy, but several unmet needs remain. Here we show that detection 
      of IgE- and IgG-reactivity to a panel of respiratory allergens microarrayed onto 
      silicon elements is more sensitive than glass-based chips. We discuss the 
      advantages of silicon-based allergen microarrays and how this technology will 
      allow addressing hitherto unmet needs in microarray-based allergy diagnosis. 
      Importantly, it described how the assembly of silicon microarray elements may 
      create different microarray formats for suiting different diagnostic applications 
      such as quick testing of single patients, medium scale testing and fully 
      automated large scale testing.
CI  - Copyright (c) 2021 Huang, Campana, Akinfenwa, Curin, Sarzsinszky, Karsonova, 
      Riabova, Karaulov, Niespodziana, Elisyutina, Fedenko, Litovkina, Smolnikov, 
      Khaitov, Vrtala, Schlederer and Valenta.
FAU - Huang, Huey-Jy
AU  - Huang HJ
AD  - Division of Immunopathology, Department of Pathophysiology and Allergy Research, 
      Center for Pathophysiology, Infectiology and Immunology, Medical University of 
      Vienna, Vienna, Austria.
FAU - Campana, Raffaela
AU  - Campana R
AD  - Division of Immunopathology, Department of Pathophysiology and Allergy Research, 
      Center for Pathophysiology, Infectiology and Immunology, Medical University of 
      Vienna, Vienna, Austria.
FAU - Akinfenwa, Oluwatoyin
AU  - Akinfenwa O
AD  - Division of Immunopathology, Department of Pathophysiology and Allergy Research, 
      Center for Pathophysiology, Infectiology and Immunology, Medical University of 
      Vienna, Vienna, Austria.
FAU - Curin, Mirela
AU  - Curin M
AD  - Division of Immunopathology, Department of Pathophysiology and Allergy Research, 
      Center for Pathophysiology, Infectiology and Immunology, Medical University of 
      Vienna, Vienna, Austria.
FAU - Sarzsinszky, Eszter
AU  - Sarzsinszky E
AD  - Division of Immunopathology, Department of Pathophysiology and Allergy Research, 
      Center for Pathophysiology, Infectiology and Immunology, Medical University of 
      Vienna, Vienna, Austria.
FAU - Karsonova, Antonina
AU  - Karsonova A
AD  - Laboratory of Immunopathology, Department of Clinical Immunology and Allergology, 
      Sechenov First Moscow State Medical University, Moscow, Russia.
FAU - Riabova, Ksenja
AU  - Riabova K
AD  - Laboratory of Immunopathology, Department of Clinical Immunology and Allergology, 
      Sechenov First Moscow State Medical University, Moscow, Russia.
FAU - Karaulov, Alexander
AU  - Karaulov A
AD  - Laboratory of Immunopathology, Department of Clinical Immunology and Allergology, 
      Sechenov First Moscow State Medical University, Moscow, Russia.
FAU - Niespodziana, Katarzyna
AU  - Niespodziana K
AD  - Division of Immunopathology, Department of Pathophysiology and Allergy Research, 
      Center for Pathophysiology, Infectiology and Immunology, Medical University of 
      Vienna, Vienna, Austria.
FAU - Elisyutina, Olga
AU  - Elisyutina O
AD  - Department of Allergology and Clinical Immunology, NRC Institute of Immunology 
      FMBA of Russia, Moscow, Russia.
FAU - Fedenko, Elena
AU  - Fedenko E
AD  - Department of Allergology and Clinical Immunology, NRC Institute of Immunology 
      FMBA of Russia, Moscow, Russia.
FAU - Litovkina, Alla
AU  - Litovkina A
AD  - Department of Allergology and Clinical Immunology, NRC Institute of Immunology 
      FMBA of Russia, Moscow, Russia.
FAU - Smolnikov, Evgenii
AU  - Smolnikov E
AD  - Department of Allergology and Clinical Immunology, NRC Institute of Immunology 
      FMBA of Russia, Moscow, Russia.
FAU - Khaitov, Musa
AU  - Khaitov M
AD  - Department of Allergology and Clinical Immunology, NRC Institute of Immunology 
      FMBA of Russia, Moscow, Russia.
FAU - Vrtala, Susanne
AU  - Vrtala S
AD  - Division of Immunopathology, Department of Pathophysiology and Allergy Research, 
      Center for Pathophysiology, Infectiology and Immunology, Medical University of 
      Vienna, Vienna, Austria.
FAU - Schlederer, Thomas
AU  - Schlederer T
AD  - Division of Immunopathology, Department of Pathophysiology and Allergy Research, 
      Center for Pathophysiology, Infectiology and Immunology, Medical University of 
      Vienna, Vienna, Austria.
FAU - Valenta, Rudolf
AU  - Valenta R
AD  - Division of Immunopathology, Department of Pathophysiology and Allergy Research, 
      Center for Pathophysiology, Infectiology and Immunology, Medical University of 
      Vienna, Vienna, Austria.
AD  - Laboratory of Immunopathology, Department of Clinical Immunology and Allergology, 
      Sechenov First Moscow State Medical University, Moscow, Russia.
AD  - Department of Allergology and Clinical Immunology, NRC Institute of Immunology 
      FMBA of Russia, Moscow, Russia.
AD  - Karl Landsteiner University of Health Sciences, Krems, Austria.
LA  - eng
GR  - P 29398/FWF_/Austrian Science Fund FWF/Austria
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
DEP - 20210212
PL  - Switzerland
TA  - Front Immunol
JT  - Frontiers in immunology
JID - 101560960
RN  - 0 (Allergens)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Allergens/*chemistry
MH  - Humans
MH  - Hypersensitivity/*blood/*diagnosis
MH  - Immunoglobulin E/*blood
MH  - *Protein Array Analysis
PMC - PMC7928321
OTO - NOTNLM
OT  - IgE
OT  - allergen
OT  - allergen chip
OT  - allergy
OT  - microarrayed allergens
OT  - molecular diagnosis
OT  - precision medicine
COIS- RV has received research grants from HVD Life Science, Vienna, Austria, and 
      Viravaxx, Vienna, Austria, and serves as a consultant for Viravaxx. The remaining 
      authors declare that the research was conducted in the absence of any commercial 
      or financial relationships that could be construed as a potential conflict of 
      interest.
EDAT- 2021/03/09 06:00
MHDA- 2021/06/22 06:00
PMCR- 2020/01/01
CRDT- 2021/03/08 05:50
PHST- 2020/08/20 00:00 [received]
PHST- 2020/12/08 00:00 [accepted]
PHST- 2021/03/08 05:50 [entrez]
PHST- 2021/03/09 06:00 [pubmed]
PHST- 2021/06/22 06:00 [medline]
PHST- 2020/01/01 00:00 [pmc-release]
AID - 10.3389/fimmu.2020.594978 [doi]
PST - epublish
SO  - Front Immunol. 2021 Feb 12;11:594978. doi: 10.3389/fimmu.2020.594978. eCollection 
      2020.