PMID- 33679843
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210310
IS  - 1664-462X (Print)
IS  - 1664-462X (Electronic)
IS  - 1664-462X (Linking)
VI  - 12
DP  - 2021
TI  - Application of Phenotyping Methods in Detection of Drought and Salinity Stress in 
      Basil (Ocimum basilicum L.).
PG  - 629441
LID - 10.3389/fpls.2021.629441 [doi]
LID - 629441
AB  - Basil is one of the most widespread aromatic and medicinal plants, which is often 
      grown in drought- and salinity-prone regions. Often co-occurrence of drought and 
      salinity stresses in agroecosystems and similarities of symptoms which they cause 
      on plants complicates the differentiation among them. Development of automated 
      phenotyping techniques with integrative and simultaneous quantification of 
      multiple morphological and physiological traits enables early detection and 
      quantification of different stresses on a whole plant basis. In this study, we 
      have used different phenotyping techniques including chlorophyll fluorescence 
      imaging, multispectral imaging, and 3D multispectral scanning, aiming to quantify 
      changes in basil phenotypic traits under early and prolonged drought and salinity 
      stress and to determine traits which could differentiate among drought and 
      salinity stressed basil plants. Ocimum basilicum "Genovese" was grown in a growth 
      chamber under well-watered control [45-50% volumetric water content (VWC)], 
      moderate salinity stress (100 mM NaCl), severe salinity stress (200 mM NaCl), 
      moderate drought stress (25-30% VWC), and severe drought stress (15-20% VWC). 
      Phenotypic traits were measured for 3 weeks in 7-day intervals. Automated 
      phenotyping techniques were able to detect basil responses to early and prolonged 
      salinity and drought stress. In addition, several phenotypic traits were able to 
      differentiate among salinity and drought. At early stages, low anthocyanin index 
      (ARI), chlorophyll index (CHI), and hue (HUE(2) (D) ), and higher reflectance in 
      red (R (Red) ), reflectance in green (R (Green) ), and leaf inclination (LINC) 
      indicated drought stress. At later stress stages, maximum fluorescence (F (m) ), 
      HUE(2) (D) , normalized difference vegetation index (NDVI), and LINC contribute 
      the most to the differentiation among drought and non-stressed as well as among 
      drought and salinity stressed plants. ARI and electron transport rate (ETR) were 
      best for differentiation of salinity stressed plants from non-stressed plants 
      both at early and prolonged stress.
CI  - Copyright (c) 2021 Lazarevic, Satovic, Nimac, Vidak, Gunjaca, Politeo and 
      Carovic-Stanko.
FAU - Lazarevic, Boris
AU  - Lazarevic B
AD  - Department of Plant Nutrition, Faculty of Agriculture, University of Zagreb, 
      Zagreb, Croatia.
AD  - Centre of Excellence for Biodiversity and Molecular Plant Breeding (CroP-BioDiv), 
      Faculty of Agriculture, University of Zagreb, Zagreb, Croatia.
FAU - Satovic, Zlatko
AU  - Satovic Z
AD  - Centre of Excellence for Biodiversity and Molecular Plant Breeding (CroP-BioDiv), 
      Faculty of Agriculture, University of Zagreb, Zagreb, Croatia.
AD  - Department of Seed Science and Technology, Faculty of Agriculture, University of 
      Zagreb, Zagreb, Croatia.
FAU - Nimac, Ana
AU  - Nimac A
AD  - Centre of Excellence for Biodiversity and Molecular Plant Breeding (CroP-BioDiv), 
      Faculty of Agriculture, University of Zagreb, Zagreb, Croatia.
FAU - Vidak, Monika
AU  - Vidak M
AD  - Centre of Excellence for Biodiversity and Molecular Plant Breeding (CroP-BioDiv), 
      Faculty of Agriculture, University of Zagreb, Zagreb, Croatia.
FAU - Gunjaca, Jerko
AU  - Gunjaca J
AD  - Centre of Excellence for Biodiversity and Molecular Plant Breeding (CroP-BioDiv), 
      Faculty of Agriculture, University of Zagreb, Zagreb, Croatia.
AD  - Department of Plant Breeding, Genetics and Biometrics, Faculty of Agriculture, 
      University of Zagreb, Zagreb, Croatia.
FAU - Politeo, Olivera
AU  - Politeo O
AD  - Department of Biochemistry, Faculty of Chemistry and Technology, University of 
      Split, Split, Croatia.
FAU - Carovic-Stanko, Klaudija
AU  - Carovic-Stanko K
AD  - Centre of Excellence for Biodiversity and Molecular Plant Breeding (CroP-BioDiv), 
      Faculty of Agriculture, University of Zagreb, Zagreb, Croatia.
AD  - Department of Seed Science and Technology, Faculty of Agriculture, University of 
      Zagreb, Zagreb, Croatia.
LA  - eng
PT  - Journal Article
DEP - 20210218
PL  - Switzerland
TA  - Front Plant Sci
JT  - Frontiers in plant science
JID - 101568200
PMC - PMC7929983
OTO - NOTNLM
OT  - 3D scanning
OT  - chlorophyll fluorescence imaging
OT  - drought stress
OT  - high-throughput phenotyping
OT  - salinity stress
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2021/03/09 06:00
MHDA- 2021/03/09 06:01
PMCR- 2021/01/01
CRDT- 2021/03/08 05:50
PHST- 2020/11/14 00:00 [received]
PHST- 2021/01/25 00:00 [accepted]
PHST- 2021/03/08 05:50 [entrez]
PHST- 2021/03/09 06:00 [pubmed]
PHST- 2021/03/09 06:01 [medline]
PHST- 2021/01/01 00:00 [pmc-release]
AID - 10.3389/fpls.2021.629441 [doi]
PST - epublish
SO  - Front Plant Sci. 2021 Feb 18;12:629441. doi: 10.3389/fpls.2021.629441. 
      eCollection 2021.