PMID- 33680886
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220421
IS  - 2228-5482 (Print)
IS  - 2251-676X (Electronic)
IS  - 2228-5482 (Linking)
VI  - 22
IP  - 1
DP  - 2021 Jan-Mar
TI  - Frequency of Sperm Aneuploidy in Oligoasthenoteratozoospermic (OAT) Patients by 
      Comprehensive Chromosome Screening: A Proof of Concept.
PG  - 57-64
LID - 10.18502/jri.v22i1.4996 [doi]
AB  - BACKGROUND: Embryonic aneuploidy usually results in implantation failure and 
      miscarriage. Considering significantly high frequency of sperm aneuploidy 
      reported in oligoasthenoteratozoospermia (OAT) using fluorescence in situ 
      hybridization (FISH) in limited number of chromosomes and lack of comprehensive 
      chromosome screening (CCS) in OAT, the aim of this study was applying CCS in OAT 
      sperm and comparison of the results with FISH findings. METHODS: Five OAT 
      patients with normal blood karyotypes and history of implantation failure were 
      included. The successfully amplified samples, each containing two sperm, were 
      analyzed by array comparative genomic hybridization (aCGH). FISH was utilized 
      mainly depending on the aneuploidies found by aCGH to assess their frequencies in 
      total sperm population. RESULTS: In aCGH for 30 sperm, aneuploidy was found in 
      66% of samples. Following the study of 4300 sperm by FISH, an average of 55.46% 
      aneuploidy was observed. No pregnancy was resulted with normal partners. 
      CONCLUSION: Using aCGH, some abnormalities were observed that are not typically 
      considered in sperm FISH studies. Despite small sample size of the comprehensive 
      study, like other similar studies, the frequency of aneuploidies was considerable 
      and similar to FISH. Aneuploidies revealed by aCGH at single sperm resolution 
      were different from sperm population detected by FISH. Considering high frequency 
      of aneuploidy in OATs sperm, preimplantation genetic testing for aneuploidy 
      (PGT-A) can be used for in transfer of chromosomally normal embryos.
CI  - Copyright(c) 2021, Avicenna Research Institute.
FAU - Saei, Parishad
AU  - Saei P
AD  - Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute 
      for Reproductive Biomedicine, ACECR, Tehran, Iran.
AD  - Department of Molecular Genetics, Faculty of Basic Sciences and Advanced 
      Technologies in Biology, University of Science and Culture, ACECR, Tehran, Iran.
FAU - Bazrgar, Masood
AU  - Bazrgar M
AD  - Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute 
      for Reproductive Biomedicine, ACECR, Tehran, Iran.
FAU - Gourabi, Hamid
AU  - Gourabi H
AD  - Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute 
      for Reproductive Biomedicine, ACECR, Tehran, Iran.
FAU - Kariminejad, Roxana
AU  - Kariminejad R
AD  - Kariminejad-Najmabadi Pathology and Genetics Center, Tehran, Iran.
FAU - Eftekhari-Yazdi, Poopak
AU  - Eftekhari-Yazdi P
AD  - Department of Embryology, Reproductive Biomedicine Research Center, Royan 
      Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
FAU - Fakhri, Mostafa
AU  - Fakhri M
AD  - Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute 
      for Reproductive Biomedicine, ACECR, Tehran, Iran.
LA  - eng
PT  - Journal Article
PL  - Iran
TA  - J Reprod Infertil
JT  - Journal of reproduction & infertility
JID - 101535586
PMC - PMC7903664
OTO - NOTNLM
OT  - Aneuploidy
OT  - Array comparative genomic hybridization
OT  - Fluorescence
OT  - In situ hybridization
OT  - Oligospermia
OT  - Spermatozoa
COIS- Conflict of Interest The authors declare that they have no conflict of interests 
      to disclose.
EDAT- 2021/03/09 06:00
MHDA- 2021/03/09 06:01
PMCR- 2021/01/01
CRDT- 2021/03/08 05:54
PHST- 2021/03/08 05:54 [entrez]
PHST- 2021/03/09 06:00 [pubmed]
PHST- 2021/03/09 06:01 [medline]
PHST- 2021/01/01 00:00 [pmc-release]
AID - JRI-22-57 [pii]
AID - 10.18502/jri.v22i1.4996 [doi]
PST - ppublish
SO  - J Reprod Infertil. 2021 Jan-Mar;22(1):57-64. doi: 10.18502/jri.v22i1.4996.