PMID- 33680908
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210310
IS  - 2234-943X (Print)
IS  - 2234-943X (Electronic)
IS  - 2234-943X (Linking)
VI  - 10
DP  - 2020
TI  - Down-Regulation of MiR-181c-5p Promotes Epithelial-to-Mesenchymal Transition in 
      Laryngeal Squamous Cell Carcinoma via Targeting SERPINE1.
PG  - 544476
LID - 10.3389/fonc.2020.544476 [doi]
LID - 544476
AB  - Laryngeal squamous cell carcinoma (LSCC) arises from the squamous epithelium of 
      the larynx and is associated with a high incidence of cervical lymph node 
      metastasis. MicroRNAs (miRNAs) play a crucial role in the epigenetic regulation 
      of cellular biological processes, including cancer metastasis. However, the 
      molecular mechanisms of specific miRNAs responsible for LSCC metastasis and their 
      clinical significance have yet to be fully elucidated. In this study, LSCC cohort 
      datasets from the Gene Expression Omnibus (GEO) and The Cancer Genome Atlas 
      (TCGA) were downloaded and examined by comprehensive bioinformatics analysis, 
      which revealed that upregulation of mRNA SERPINE1 and downregulation of 
      miR-181c-5p were associated with unfavorable overall survival. Our analysis 
      showed that SERPINE1 expression negatively correlated with the expression level 
      of miR-181c-5p in our LSCC patient samples. Silencing of miR-181c-5p expression 
      promoted cell migration and invasion in cell lines, whereas the overexpression of 
      miR-181c-5p suppressed cell migration and epithelial-to-mesenchymal transition 
      (EMT) through the downregulation of SERPINE1. Further analysis showed that the 
      enhancement effect on EMT and metastasis induced by silencing miR-181c-5p could 
      be rescued through knockdown of SERPINE1 expression in vitro. Collectively, our 
      findings indicated that miR-181c-5p acted as an EMT suppressor miRNA by 
      downregulation of SERPINE1 in LSCC and offers novel strategies for the prevention 
      of metastasis in LSCC.
CI  - Copyright (c) 2020 Li, Wu, Tang, Fan, Liu, Fang, Wang and Zhao.
FAU - Li, Xin
AU  - Li X
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Xiangya Hospital of 
      Central South University, Changsha, China.
AD  - Province Key Laboratory of Otolaryngology Critical Diseases, Xiangya Hospital of 
      Central South University, Changsha, China.
AD  - National Clinical Research Center for Geriatric Disorders, Xiangya Hospital of 
      Central South University, Changsha, China.
FAU - Wu, Ping
AU  - Wu P
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Xiangya Hospital of 
      Central South University, Changsha, China.
AD  - Province Key Laboratory of Otolaryngology Critical Diseases, Xiangya Hospital of 
      Central South University, Changsha, China.
AD  - National Clinical Research Center for Geriatric Disorders, Xiangya Hospital of 
      Central South University, Changsha, China.
FAU - Tang, Yaoyun
AU  - Tang Y
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Xiangya Hospital of 
      Central South University, Changsha, China.
AD  - Province Key Laboratory of Otolaryngology Critical Diseases, Xiangya Hospital of 
      Central South University, Changsha, China.
AD  - National Clinical Research Center for Geriatric Disorders, Xiangya Hospital of 
      Central South University, Changsha, China.
FAU - Fan, Yuhua
AU  - Fan Y
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Xiangya Hospital of 
      Central South University, Changsha, China.
AD  - Province Key Laboratory of Otolaryngology Critical Diseases, Xiangya Hospital of 
      Central South University, Changsha, China.
AD  - National Clinical Research Center for Geriatric Disorders, Xiangya Hospital of 
      Central South University, Changsha, China.
FAU - Liu, Yalan
AU  - Liu Y
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Xiangya Hospital of 
      Central South University, Changsha, China.
AD  - Province Key Laboratory of Otolaryngology Critical Diseases, Xiangya Hospital of 
      Central South University, Changsha, China.
AD  - National Clinical Research Center for Geriatric Disorders, Xiangya Hospital of 
      Central South University, Changsha, China.
FAU - Fang, Xing
AU  - Fang X
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Xiangya Hospital of 
      Central South University, Changsha, China.
AD  - Province Key Laboratory of Otolaryngology Critical Diseases, Xiangya Hospital of 
      Central South University, Changsha, China.
AD  - National Clinical Research Center for Geriatric Disorders, Xiangya Hospital of 
      Central South University, Changsha, China.
FAU - Wang, Wei
AU  - Wang W
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Xiangya Hospital of 
      Central South University, Changsha, China.
AD  - Province Key Laboratory of Otolaryngology Critical Diseases, Xiangya Hospital of 
      Central South University, Changsha, China.
AD  - National Clinical Research Center for Geriatric Disorders, Xiangya Hospital of 
      Central South University, Changsha, China.
FAU - Zhao, Suping
AU  - Zhao S
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Xiangya Hospital of 
      Central South University, Changsha, China.
AD  - Province Key Laboratory of Otolaryngology Critical Diseases, Xiangya Hospital of 
      Central South University, Changsha, China.
AD  - National Clinical Research Center for Geriatric Disorders, Xiangya Hospital of 
      Central South University, Changsha, China.
LA  - eng
PT  - Journal Article
DEP - 20201221
PL  - Switzerland
TA  - Front Oncol
JT  - Frontiers in oncology
JID - 101568867
PMC - PMC7931772
OTO - NOTNLM
OT  - biomarker
OT  - epithelial-to-mesenchymal transition
OT  - laryngeal squamous cell carcinoma
OT  - mRNA
OT  - miRNA
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2021/03/09 06:00
MHDA- 2021/03/09 06:01
PMCR- 2020/01/01
CRDT- 2021/03/08 05:54
PHST- 2020/03/20 00:00 [received]
PHST- 2020/11/18 00:00 [accepted]
PHST- 2021/03/08 05:54 [entrez]
PHST- 2021/03/09 06:00 [pubmed]
PHST- 2021/03/09 06:01 [medline]
PHST- 2020/01/01 00:00 [pmc-release]
AID - 10.3389/fonc.2020.544476 [doi]
PST - epublish
SO  - Front Oncol. 2020 Dec 21;10:544476. doi: 10.3389/fonc.2020.544476. eCollection 
      2020.