PMID- 33683044
OWN - NLM
STAT- MEDLINE
DCOM- 20211103
LR  - 20211103
IS  - 1812-5735 (Electronic)
IS  - 1028-8880 (Linking)
VI  - 24
IP  - 1
DP  - 2021 Jan
TI  - Effect of Nano Herbal Andaliman (Zanthoxylum acanthopodium) Fruits in NOTCH1 and 
      Hes1 Expressions to Human Placental Trophoblasts.
PG  - 165-171
LID - 10.3923/pjbs.2021.165.171 [doi]
AB  - BACKGROUND AND OBJECTIVE: Andaliman fruit (Zanthoxylum acanthopodium) is a 
      well-known spice antioxidant in Northern Sumatera (Indonesia). The cellular 
      activity requires antioxidants in counteracting free radicals. The cellular 
      proteins that play a role in development, proliferation, differentiation and 
      embryonic processes in the human placenta are NOTCH1 and Hes1. The aim of this 
      research was to analyze the expression of NOTCH1 and Hes1 genes after 
      administering nano herbal andaliman to the trophoblast cells of the human 
      placenta. MATERIALS AND METHODS: HTR8 trophoblast cells were divided into two 
      groups, namely, control and treatment (nano herbal andaliman). RNA isolation, 
      reverse transcription and RT-PCR (real-time polymerase chain reaction) were 
      performed to analyze the NOTCH1 and Notch target gene (Hes1) expressions. The 
      NOTCH1 and Hes1 gene expressions were quantified using the CT method (2-DeltaDeltaCT) and 
      normalized with Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene 
      expressions. RESULTS: Nanoherbal andaliman reduced the expression of NOTCH1 genes 
      in the human placental trophoblast. However, it increased the expression of Hes1 
      when the incubation time was 16 hrs. CONCLUSION: Nanoherbal andaliman decreases 
      the expression of genes that are crucial in hypoxia and free radicals in the 
      placenta, namely, NOTCH1 and Hes1 increased after incubation for 16 hrs. 
      Therefore, this herb needs to be evaluated further.
FAU - C Situmorang, Putri
AU  - C Situmorang P
FAU - Ilyas, Syafruddin
AU  - Ilyas S
FAU - Hutahaean, Salomo
AU  - Hutahaean S
FAU - Rosidah
AU  - Rosidah
LA  - eng
PT  - Journal Article
PL  - Pakistan
TA  - Pak J Biol Sci
JT  - Pakistan journal of biological sciences : PJBS
JID - 101247723
RN  - 0 (NOTCH1 protein, human)
RN  - 0 (Plant Extracts)
RN  - 0 (Receptor, Notch1)
RN  - 0 (Transcription Factor HES-1)
RN  - 149348-15-2 (HES1 protein, human)
SB  - IM
MH  - Cell Line
MH  - Female
MH  - *Fruit/chemistry
MH  - Gene Expression Regulation
MH  - Humans
MH  - *Nanoparticles
MH  - Plant Extracts/isolation & purification/*pharmacology
MH  - Receptor, Notch1/genetics/*metabolism
MH  - Time Factors
MH  - Transcription Factor HES-1/genetics/*metabolism
MH  - Trophoblasts/*drug effects/metabolism
MH  - *Zanthoxylum/chemistry
OTO - NOTNLM
OT  - Andaliman
OT  - HTR-8 cells
OT  - Hes1
OT  - NOTCH1
OT  - Zanthoxylum acanthopodium
OT  - nano herbal
OT  - trophoblast
EDAT- 2021/03/09 06:00
MHDA- 2021/11/04 06:00
CRDT- 2021/03/08 08:47
PHST- 2021/03/08 08:47 [entrez]
PHST- 2021/03/09 06:00 [pubmed]
PHST- 2021/11/04 06:00 [medline]
AID - 10.3923/pjbs.2021.165.171 [doi]
PST - ppublish
SO  - Pak J Biol Sci. 2021 Jan;24(1):165-171. doi: 10.3923/pjbs.2021.165.171.