PMID- 33683140
OWN - NLM
STAT- MEDLINE
DCOM- 20211125
LR  - 20220531
IS  - 1552-9924 (Electronic)
IS  - 0091-6765 (Print)
IS  - 0091-6765 (Linking)
VI  - 129
IP  - 3
DP  - 2021 Mar
TI  - Aggressiveness and Metastatic Potential of Breast Cancer Cells Co-Cultured with 
      Preadipocytes and Exposed to an Environmental Pollutant Dioxin: An in Vitro and 
      in Vivo Zebrafish Study.
PG  - 37002
LID - 10.1289/EHP7102 [doi]
LID - 037002
AB  - BACKGROUND: Breast cancer (BC) is a major public health concern, and its 
      prognosis is very poor once metastasis occurs. The tumor microenvironment and 
      chemical pollution have been suggested recently to contribute, independently, to 
      the development of metastatic cells. The BC microenvironment consists, in part, 
      of adipocytes and preadipocytes in which persistent organic pollutants (POPs) can 
      be stored. OBJECTIVES: We aimed to test the hypothesis that these two factors 
      (2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), an extensively studied, toxic POP 
      and the microenvironment) may interact to increase tumor aggressiveness. METHODS: 
      We used a co-culture model using BC MCF-7 cells or MDA-MB-231 cells together with 
      hMADS preadipocytes to investigate the contribution of the microenvironment and 
      2,3,7,8-tetrachlorodibenzo-p-dioxin TCDD on BC cells. Global differences were 
      characterized using a high-throughput proteomic assay. Subsequently we measured 
      the BC stem cell-like activity, analyzed the cell morphology, and used a 
      zebrafish larvae model to study the metastatic potential of the BC cells. 
      RESULTS: We found that coexposure to TCDD and preadipocytes modified BC cell 
      properties; moreover, it induced the expression of ALDH1A3, a cancer stem cell 
      marker, and the appearance of giant cancer cells with cell-in-cell structures 
      (CICs), which are associated with malignant metastatic progression, that we 
      demonstrated in vivo. DISCUSSION: The results of our study using BC cell lines 
      co-cultured with preadipocytes and a POP and an in vivo zebrafish model of 
      metastasis suggest that the interactions between BC cells and their 
      microenvironment could affect their invasive or metastatic potential. 
      https://doi.org/10.1289/EHP7102.
FAU - Koual, Meriem
AU  - Koual M
AD  - UMR-S1124, Institut national de la sante et de la recherche medicale (Inserm), 
      T3S, Toxicologie Environnementale, Cibles therapeutiques, Signalisation 
      cellulaire et Biomarqueurs, Paris, France.
AD  - Service de Chirurgie Cancerologique Gynecologique et du Sein, Hopital Europeen 
      Georges-Pompidou, Assistance Publique-Hopitaux de Paris, France.
AD  - Universite de Paris, Paris, France.
FAU - Tomkiewicz, Celine
AU  - Tomkiewicz C
AD  - UMR-S1124, Institut national de la sante et de la recherche medicale (Inserm), 
      T3S, Toxicologie Environnementale, Cibles therapeutiques, Signalisation 
      cellulaire et Biomarqueurs, Paris, France.
FAU - Guerrera, Ida Chiara
AU  - Guerrera IC
AD  - Plateforme proteomique 3P5-Necker, Structure Federative de Recherche Necker, 
      Universite de Paris, US24/CNRS UMS3633, Inserm, Paris, France.
FAU - Sherr, David
AU  - Sherr D
AD  - Department of Environmental Health, Boston University School of Public Health, 
      Boston, Massachusetts, USA.
FAU - Barouki, Robert
AU  - Barouki R
AD  - UMR-S1124, Institut national de la sante et de la recherche medicale (Inserm), 
      T3S, Toxicologie Environnementale, Cibles therapeutiques, Signalisation 
      cellulaire et Biomarqueurs, Paris, France.
AD  - Universite de Paris, Paris, France.
FAU - Coumoul, Xavier
AU  - Coumoul X
AD  - UMR-S1124, Institut national de la sante et de la recherche medicale (Inserm), 
      T3S, Toxicologie Environnementale, Cibles therapeutiques, Signalisation 
      cellulaire et Biomarqueurs, Paris, France.
AD  - Universite de Paris, Paris, France.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210308
PL  - United States
TA  - Environ Health Perspect
JT  - Environmental health perspectives
JID - 0330411
RN  - 0 (Dioxins)
RN  - 0 (Environmental Pollutants)
RN  - 0 (Polychlorinated Dibenzodioxins)
SB  - IM
MH  - Animals
MH  - *Breast Neoplasms/chemically induced
MH  - Coculture Techniques
MH  - *Dioxins
MH  - *Environmental Pollutants
MH  - Female
MH  - Humans
MH  - MCF-7 Cells
MH  - *Polychlorinated Dibenzodioxins
MH  - Proteomics
MH  - Tumor Microenvironment
MH  - Zebrafish
PMC - PMC7939125
EDAT- 2021/03/09 06:00
MHDA- 2021/11/26 06:00
PMCR- 2021/03/08
CRDT- 2021/03/08 12:12
PHST- 2021/03/08 12:12 [entrez]
PHST- 2021/03/09 06:00 [pubmed]
PHST- 2021/11/26 06:00 [medline]
PHST- 2021/03/08 00:00 [pmc-release]
AID - EHP7102 [pii]
AID - 10.1289/EHP7102 [doi]
PST - ppublish
SO  - Environ Health Perspect. 2021 Mar;129(3):37002. doi: 10.1289/EHP7102. Epub 2021 
      Mar 8.