PMID- 33684694
OWN - NLM
STAT- MEDLINE
DCOM- 20210722
LR  - 20210722
IS  - 1950-6007 (Electronic)
IS  - 0753-3322 (Linking)
VI  - 138
DP  - 2021 Jun
TI  - Augmentation of anti-proliferative, pro-apoptotic and oxidant profiles induced by 
      piceatannol in human breast carcinoma MCF-7 cells using zein nanostructures.
PG  - 111409
LID - S0753-3322(21)00194-3 [pii]
LID - 10.1016/j.biopha.2021.111409 [doi]
AB  - Piceatannol (PCT), a natural polyphenolic stilbene, has pleiotropic 
      pharmacological potentials. It possesses cytotoxic activities toward variant 
      cancerous cells. Zein nanospheres (ZN NSs) have been introduced as ideal 
      nanostructures due to their natural origin, safety, histocompatibility. and 
      convenient method of formulation. The purpose of this study was to explore the 
      impact of PCT-ZN NSs formula on pharmacotherapy potential of PCT against human 
      breast cancer MCF-7 cells. PCT-ZN NSs were formulated and characterized 
      selectively to particle size, zeta potential, encapsulation efficiency and 
      diffusion of PCT. The selected formula has a particle size of 84.4 +/- 2.3 nm, zeta 
      potential value of 33.8 +/- 1.2 mV and encapsulation efficiency of 89.5 +/- 4.1%. 
      PCT-ZN NSs displayed significantly lower IC(50) against MCF-7 cells by about 24 
      folds. Further, PCT-ZN NSs formula showed higher cellular uptake as compared to 
      free PCT. Examination of cell cycle phases displayed cells accumulation in G2-M 
      phase and increased percentage cells in pre-G1 phase indicating an 
      apoptosis-enhancing activity. Annexin V staining indicated augmented early and 
      late apoptosis. PCT-ZN NSs pro-apoptotic activity was confirmed by the observed 
      significant increased mRNA expression of CASP3, p53, and Bax as well as decreased 
      expression of Bcl2. In addition, PCT-ZN NSs induced oxidative stress as evidenced 
      by depletion of glutathione reductase (GR) activity, increased generation of 
      reactive oxygen species (ROS) and accumulation of lipid peroxidation products. 
      Conclusively, ZN nanostructures of PCT revealed superior cell death-inducing 
      activities against MCF-7 cells in comparison with free PCT. This is mediated, at 
      least partly, by enhanced cellular uptake, pro-apoptotic activity, and oxidative 
      stress potential.
CI  - Copyright (c) 2021 The Authors. Published by Elsevier Masson SAS.. All rights 
      reserved.
FAU - Algandaby, Mardi M
AU  - Algandaby MM
AD  - Faculty of Science, Department of Biological Sciences, King Abdulaziz University, 
      Jeddah, Saudi Arabia; Medicinal Plants Research Group, Deanship of Scientific 
      Research, King Abdulaziz University, Jeddah, Saudi Arabia.
FAU - Al-Sawahli, Majid M
AU  - Al-Sawahli MM
AD  - Department of Pharmaceutical Technology, Faculty of Pharmacy, Kafr Elsheikh 
      University, Kafr Elsheikh 33516, Egypt. Electronic address: 
      majid.alsawahli@pharm.kfs.edu.eg.
LA  - eng
PT  - Journal Article
DEP - 20210305
PL  - France
TA  - Biomed Pharmacother
JT  - Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
JID - 8213295
RN  - 0 (Antioxidants)
RN  - 0 (Stilbenes)
RN  - 6KS3LS0D4F (3,3',4,5'-tetrahydroxystilbene)
RN  - 9010-66-6 (Zein)
SB  - IM
MH  - Antioxidants/*administration & dosage/therapeutic use
MH  - Apoptosis/drug effects/physiology
MH  - *Breast Neoplasms/drug therapy/metabolism
MH  - Cell Proliferation/drug effects/physiology
MH  - Female
MH  - Humans
MH  - MCF-7 Cells
MH  - Nanostructures/*administration & dosage/therapeutic use
MH  - Oxidative Stress/*drug effects/physiology
MH  - Stilbenes/*administration & dosage/therapeutic use
MH  - Zein/*administration & dosage/therapeutic use
OTO - NOTNLM
OT  - Apoptosis
OT  - Breast cancer
OT  - Cell cycle
OT  - Cytotoxicity
OT  - Diffusion
OT  - MCF-7
OT  - Nanospheres
OT  - Piceatannol
OT  - Zein
EDAT- 2021/03/09 06:00
MHDA- 2021/07/23 06:00
CRDT- 2021/03/08 20:13
PHST- 2020/11/01 00:00 [received]
PHST- 2021/02/06 00:00 [revised]
PHST- 2021/02/16 00:00 [accepted]
PHST- 2021/03/09 06:00 [pubmed]
PHST- 2021/07/23 06:00 [medline]
PHST- 2021/03/08 20:13 [entrez]
AID - S0753-3322(21)00194-3 [pii]
AID - 10.1016/j.biopha.2021.111409 [doi]
PST - ppublish
SO  - Biomed Pharmacother. 2021 Jun;138:111409. doi: 10.1016/j.biopha.2021.111409. Epub 
      2021 Mar 5.