PMID- 33685714
OWN - NLM
STAT- MEDLINE
DCOM- 20210414
LR  - 20210414
IS  - 1525-3198 (Electronic)
IS  - 0022-0302 (Linking)
VI  - 104
IP  - 4
DP  - 2021 Apr
TI  - The occurrence of methicillin-resistant non-aureus staphylococci in samples from 
      cows, young stock, and the environment on German dairy farms.
PG  - 4604-4614
LID - S0022-0302(21)00266-6 [pii]
LID - 10.3168/jds.2020-19704 [doi]
AB  - This study aimed to determine the occurrence of methicillin-resistant (MR) 
      non-aureus staphylococci (NAS) on 20 preselected German dairy farms. Farms were 
      selected based on the detection of methicillin-resistant Staphylococcus aureus 
      (MRSA) during previous diagnostic investigations. Bacterial culture of 
      presumptive MR-NAS was based on a 2-step enrichment method that has been 
      recommended for MRSA detection. Quarter milk samples (QMS), bulk tank milk, swab 
      samples from young stock, and environmental samples were collected for bacterial 
      culture. Methicillin-resistant NAS were detected on all study farms. The MR-NAS 
      positive test rate was 3.3% (77/2,347) in QMS, 42.1% (8/19) in bulk tank milk, 
      29.1% (59/203) in nasal swabs from milk-fed calves, 18.3% (35/191) in postweaning 
      calves, and 7.3% (14/191) in nasal swabs from prefresh heifers. In the 
      environment, MR-NAS were detected in dust samples on 25% (5/20) of the dairy 
      farms as well as in teat liners and suckers from automatic calf feeders. The 
      geometric mean somatic cell count in QMS affected by MR-NAS (183,000 cells/mL) 
      was slightly higher compared with all QMS (114,000 cells/mL). Nine MR-NAS species 
      were identified; Staph. sciuri, Staph. lentus, Staph. fleurettii, Staph. 
      epidermidis, and Staph. haemolyticus were the most common species. In addition, 
      170 NAS isolates were identified that showed reduced cefoxitin susceptibility (4 
      mg/L) but did not harbor the mecA or mecC genes. On some farms, similar mobile 
      genetic elements were detected in MR-NAS and MRSA. It was suggested that 
      resistance genes may be transferred between NAS and Staph. aureus on the 
      respective farms.
CI  - The Authors. Published by Elsevier Inc. and Fass Inc. on behalf of the American 
      Dairy Science Association(R). This is an open access article under the CC BY-NC-ND 
      license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
FAU - Schnitt, A
AU  - Schnitt A
AD  - Department of Biological Safety, German Federal Institute for Risk Assessment, 
      10589 Berlin, Germany.
FAU - Lienen, T
AU  - Lienen T
AD  - Department of Biological Safety, German Federal Institute for Risk Assessment, 
      10589 Berlin, Germany.
FAU - Wichmann-Schauer, H
AU  - Wichmann-Schauer H
AD  - Department of Biological Safety, German Federal Institute for Risk Assessment, 
      10589 Berlin, Germany.
FAU - Tenhagen, B-A
AU  - Tenhagen BA
AD  - Department of Biological Safety, German Federal Institute for Risk Assessment, 
      10589 Berlin, Germany. Electronic address: Bernd-Alois.Tenhagen@bfr.bund.de.
LA  - eng
PT  - Journal Article
DEP - 20210306
PL  - United States
TA  - J Dairy Sci
JT  - Journal of dairy science
JID - 2985126R
SB  - IM
MH  - Animals
MH  - Cattle
MH  - *Cattle Diseases
MH  - Farms
MH  - Female
MH  - Methicillin Resistance
MH  - *Methicillin-Resistant Staphylococcus aureus
MH  - Microbial Sensitivity Tests/veterinary
MH  - Milk
MH  - *Staphylococcal Infections/veterinary
MH  - Staphylococcus
OTO - NOTNLM
OT  - antimicrobial resistance
OT  - coagulase-negative staphylococci
OT  - dairy cattle
OT  - methicillin
OT  - non-aureus staphylococci
EDAT- 2021/03/10 06:00
MHDA- 2021/04/15 06:00
CRDT- 2021/03/09 05:46
PHST- 2020/09/28 00:00 [received]
PHST- 2020/12/08 00:00 [accepted]
PHST- 2021/03/10 06:00 [pubmed]
PHST- 2021/04/15 06:00 [medline]
PHST- 2021/03/09 05:46 [entrez]
AID - S0022-0302(21)00266-6 [pii]
AID - 10.3168/jds.2020-19704 [doi]
PST - ppublish
SO  - J Dairy Sci. 2021 Apr;104(4):4604-4614. doi: 10.3168/jds.2020-19704. Epub 2021 
      Mar 6.