PMID- 33686088
OWN - NLM
STAT- MEDLINE
DCOM- 20211207
LR  - 20211214
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 11
IP  - 1
DP  - 2021 Mar 8
TI  - Bioinformatis analysis reveals possible molecular mechanism of PXR on regulating 
      ulcerative colitis.
PG  - 5428
LID - 10.1038/s41598-021-83742-8 [doi]
LID - 5428
AB  - Inflammatory bowel disease (IBD) is a chronic, recurrent inflammatory disease of 
      the gastrointestinal (GI) tract. Ulcerative colitis (UC) is a type of IBD. 
      Pregnane X Receptor (PXR) is a member of the nuclear receptor superfamily. In 
      order to deepen understanding and exploration of the molecular mechanism of 
      regulation roles of PXR on UC, biological informatics analysis was performed. 
      First, 878 overlapping differentially expressed genes (DEGs) between UC and 
      normal samples were obtained from the Gene Expression Omnibus (GEO) database 
      (GSE59071 and GSE38713) by using the "limma" R language package. Then WGCNA 
      analysis was performed by 878 DEGs to obtain co-expression modules that were 
      positively and negatively correlated with clinical traits. GSEA analysis of PXR 
      results obtained the signal pathways enriched in the PXR high and low expression 
      group and the active genes of each signal pathway. Then the association of PXR 
      with genes that are both active in high expression group and negatively related 
      to diseases (gene set 1), or both active in low expression group and negatively 
      related to diseases (gene set 2) was analyzed by String database. Finally, 
      carboxylesterase 2 (CES2), ATP binding cassette subfamily G member 2 (ABCG2), 
      phosphoenolpyruvate carboxykinase (PCK1), PPARG coactivator 1 alpha (PPARGC1A), 
      cytochrome P450 family 2 subfamily B member 6 (CYP2B6) from gene set 1 and C-X-C 
      motif chemokine ligand 8 (CXCL8) from gene set 2 were screened out. After the 
      above analysis and reverse transcriptase quantitative polymerase chain reaction 
      (RT-qPCR) verification, we speculated that PXR may exert a protective role on UC 
      by promoting CES2, ABCG2, PCK1, PPARGC1A, CYP2B6 expression and inhibiting CXCL8 
      expression in their corresponding signal pathway in intestinal tissue.
FAU - Guo, Hanze
AU  - Guo H
AD  - College of Life Sciences, Liaoning Normal University, Dalian, 116081, China.
AD  - College of Life Science and Technology, Dalian University, Dalian, 116622, China.
FAU - Chi, Yan
AU  - Chi Y
AD  - College of Life Sciences, Liaoning Normal University, Dalian, 116081, China. 
      chiyan@lnnu.edu.cn.
FAU - Chi, Naiyu
AU  - Chi N
AD  - College of Life Science and Technology, Dalian University, Dalian, 116622, China. 
      cny7566@126.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210308
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (NR1I2 protein, human)
RN  - 0 (Pregnane X Receptor)
SB  - IM
MH  - *Colitis, Ulcerative/genetics/metabolism
MH  - *Computational Biology
MH  - *Databases, Nucleic Acid
MH  - *Gene Expression Regulation
MH  - HCT116 Cells
MH  - Humans
MH  - *Pregnane X Receptor/genetics/metabolism
MH  - *Signal Transduction
PMC - PMC7940411
COIS- The authors declare no competing interests.
EDAT- 2021/03/10 06:00
MHDA- 2021/12/15 06:00
PMCR- 2021/03/08
CRDT- 2021/03/09 06:06
PHST- 2020/11/02 00:00 [received]
PHST- 2021/02/08 00:00 [accepted]
PHST- 2021/03/09 06:06 [entrez]
PHST- 2021/03/10 06:00 [pubmed]
PHST- 2021/12/15 06:00 [medline]
PHST- 2021/03/08 00:00 [pmc-release]
AID - 10.1038/s41598-021-83742-8 [pii]
AID - 83742 [pii]
AID - 10.1038/s41598-021-83742-8 [doi]
PST - epublish
SO  - Sci Rep. 2021 Mar 8;11(1):5428. doi: 10.1038/s41598-021-83742-8.