PMID- 33691363
OWN - NLM
STAT- MEDLINE
DCOM- 20210312
LR  - 20210312
IS  - 1001-9391 (Print)
IS  - 1001-9391 (Linking)
VI  - 39
IP  - 2
DP  - 2021 Feb 20
TI  - [Study on malignant transformation of MeT-5A cells induced by chrysotile].
PG  - 105-109
LID - 10.3760/cma.j.cn.121094-20200831-00508 [doi]
AB  - Objective: To study the cytotoxicity and malignant transformation ability of 
      chrysotile on MeT-5A cells. Methods: In June 2016, lactate dehydrogenase (LDH) 
      method was used to detect the cytotoxicity of chrysotile to MeT-5A cells. MeT-5A 
      cells were treated with 5 mug/cm(2) chrysotile intermittently for 24 h a time, 
      once a week and a total of 28 times. After the cells showed anchorage independent 
      growth, the cell features of malignant transformation were identified by colony 
      forming frequency in soft agar, and the soft agar colony formation rates were 
      calculated. The activities of key speed limiting enzymes of glycolysis metabolism 
      including hexokinase (HK) , phosphofructokinase (PFK) and pyruvate kinase (PK) 
      were determined by UV colorimetry. Results: Chrysotile was cytotoxic to MeT-5A 
      cells in a concentration-dependent decline. Compared with the control group, the 
      relative survival rates of MeT-5A cells were significantly decreased after 
      exposed to chrysotile at 10, 20, 40 and 80 mug/cm(2) (P<0.05) . After 28 times of 
      exposure, the growth rate of the cells in chrysotile transformed MeT-5A cells was 
      accelerated, the arrangement was disordered, the contact inhibition was lost, and 
      the double layer growth appeared, which could grow on soft agar. The colony 
      forming rate of the chrysotile transformed MeT-5A cells was 18.33 per thousand+/-2.49 per thousand. 
      Compared with the control group (0) , the difference was statistically 
      significant (P<0.01) . The activities of glycolysis related kinase including PK [ 
      (19.51+/-1.52) U/L], PFK[ (0.12+/-0.02) U/10(4) cell] and HK[ (0.26+/-0.01) U/10(4) 
      cell] were increased in the chrysotile transformed MeT-5A cells compared with 
      control group [ (25.00+/-1.04) U/L、(0.15+/-0.01) U/10(4) cell and (0.33+/-0.01) U/10(4) 
      cell] (P<0.01) . Conclusion: Chrysotile can induce malignant transformation of 
      MeT-5A cells and increase the activities of glycolysis related kinases including 
      PK, PFK and HK.
FAU - Zhang, F F
AU  - Zhang FF
AD  - Hangzhou Medical College (Zhejiang Academy of Medical Sciences) , School of 
      Public Health, Hangzhou 310013, China.
FAU - Yuan, X Y
AU  - Yuan XY
AD  - Hangzhou Medical College (Zhejiang Academy of Medical Sciences) , School of 
      Public Health, Hangzhou 310013, China.
FAU - Gao, Y N
AU  - Gao YN
AD  - Hangzhou Medical College (Zhejiang Academy of Medical Sciences) , School of 
      Public Health, Hangzhou 310013, China.
FAU - Zhang, M
AU  - Zhang M
AD  - Hangzhou Medical College (Zhejiang Academy of Medical Sciences) , School of 
      Public Health, Hangzhou 310013, China.
FAU - Yu, M
AU  - Yu M
AD  - Hangzhou Medical College (Zhejiang Academy of Medical Sciences) , School of 
      Public Health, Hangzhou 310013, China.
FAU - Yu, M
AU  - Yu M
AD  - Hangzhou Medical College (Zhejiang Academy of Medical Sciences) , School of 
      Public Health, Hangzhou 310013, China.
FAU - Xiao, Y
AU  - Xiao Y
AD  - Hangzhou Medical College (Zhejiang Academy of Medical Sciences) , School of 
      Public Health, Hangzhou 310013, China.
FAU - Ju, L
AU  - Ju L
AD  - Hangzhou Medical College (Zhejiang Academy of Medical Sciences) , School of 
      Public Health, Hangzhou 310013, China.
FAU - Huang, Y L
AU  - Huang YL
AD  - Hangzhou Medical College (Zhejiang Academy of Medical Sciences) , School of 
      Public Health, Hangzhou 310013, China.
FAU - Zhang, X
AU  - Zhang X
AD  - Hangzhou Medical College (Zhejiang Academy of Medical Sciences) , School of 
      Public Health, Hangzhou 310013, China.
FAU - Zhu, L J
AU  - Zhu LJ
AD  - Hangzhou Medical College (Zhejiang Academy of Medical Sciences) , School of 
      Public Health, Hangzhou 310013, China.
LA  - chi
GR  - LQY18H260001, LGD21C040008/Natural Science Foundation of Zhejiang Province/
GR  - 2018KY343, 2019KY360, 2020KY528, 2021KY128/Medical Health Science and Technology 
      Project of Zhejiang Provincial Health Commission/
GR  - Y202045373/Education Department Project of Zhejiang Province/
PT  - Journal Article
PL  - China
TA  - Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi
JT  - Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng 
      zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases
JID - 8410840
RN  - 0 (Asbestos, Serpentine)
RN  - EC 2.7.1.1 (Hexokinase)
RN  - EC 2.7.1.11 (Phosphofructokinase-1)
RN  - EC 2.7.1.40 (Pyruvate Kinase)
SB  - IM
MH  - *Asbestos, Serpentine/toxicity
MH  - Glycolysis
MH  - Hexokinase/metabolism
MH  - *Phosphofructokinase-1/metabolism
MH  - Pyruvate Kinase/metabolism
OTO - NOTNLM
OT  - Cell transformation
OT  - Chrysotile
OT  - Cytotoxicity
OT  - Glycolysis
OT  - Tumor
EDAT- 2021/03/11 06:00
MHDA- 2021/03/13 06:00
CRDT- 2021/03/10 21:23
PHST- 2021/03/10 21:23 [entrez]
PHST- 2021/03/11 06:00 [pubmed]
PHST- 2021/03/13 06:00 [medline]
AID - 10.3760/cma.j.cn.121094-20200831-00508 [doi]
PST - ppublish
SO  - Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2021 Feb 20;39(2):105-109. doi: 
      10.3760/cma.j.cn.121094-20200831-00508.