PMID- 33693294
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210312
IS  - 2434-0790 (Electronic)
IS  - 2434-0790 (Linking)
VI  - 3
IP  - 2
DP  - 2021 Jan 16
TI  - Phosphorylcholine-Primed Dendritic Cells Aggravate the Development of 
      Atherosclerosis in ApoE(-/-) Mice.
PG  - 86-94
LID - 10.1253/circrep.CR-20-0118 [doi]
AB  - Background: Atherosclerosis is an inflammatory disease involving activation of 
      adaptive and innate immune responses to antigens, including oxidized low-density 
      lipoprotein (oxLDL) and phosphorylcholine (PC). Dendritic cells (DCs), which are 
      antigen-presenting cells that activate T cells, are present in atherosclerotic 
      lesions and are activated in immune organs. However, the mechanism by which PC 
      promotes atherosclerosis is unclear. Methods and Results: To evaluate whether PC 
      promotes atherosclerosis via DCs, 2x10(5) DCs activated by PC-keyhole limpet 
      hemocyanin (DCs+PC-KLH) were injected into ApoE(-/-) mice and the features of the 
      plaques and the effects of the DCs on cellular and humoral immunity against 
      PC-KLH were determined. Mice injected with DCs+PC-KLH had significantly larger 
      atherosclerotic lesions than controls, with increased inflammation in the lesions 
      and plaque instability. Furthermore, DCs+PC-KLH were characterized using flow 
      cytometry after coculture of bone marrow-derived DCs and naive T cells. 
      DCs+PC-KLH showed an inflammatory phenotype, with increased CD86, CD40, and major 
      histocompatibility complex Class II molecules (MHC-II), which promoted 
      PC-specific T helper (Th) 1 and Th17 cell differentiation in vivo and in vitro. 
      Moreover, 2 weeks after the administration of DCs+PC-KLH to mice, these mice 
      produced PC- and oxLDL-specific IgG2a, compared with no production in the 
      controls. Conclusions: These findings suggest that DCs presenting PC promote 
      specific immunity to PC, increase lesion inflammation, and accelerate 
      atherosclerosis, which may explain how PC promotes atherosclerosis.
CI  - Copyright (c) 2021, THE JAPANESE CIRCULATION SOCIETY.
FAU - Dong, Qian
AU  - Dong Q
AD  - Department of Cardiology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology Wuhan China.
FAU - Yu, Jian
AU  - Yu J
AD  - Department of Cardiology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology Wuhan China.
FAU - Ding, Yan
AU  - Ding Y
AD  - Department of Cardiology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology Wuhan China.
FAU - Ji, Qing-Wei
AU  - Ji QW
AD  - Department of Cardiology, the People's Hospital of Guangxi Zhuang Autonomous 
      Region Nanning China.
FAU - Zhu, Rui-Rui
AU  - Zhu RR
AD  - Department of Cardiology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology Wuhan China.
FAU - Wei, Yu-Zhen
AU  - Wei YZ
AD  - Department of Cardiology, the First Affiliated Hospital of Zhengzhou University 
      Zhengzhou China.
FAU - Xu, Wen-Bing
AU  - Xu WB
AD  - Department of Cardiology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology Wuhan China.
FAU - Zhong, Yu-Cheng
AU  - Zhong YC
AD  - Department of Cardiology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology Wuhan China.
FAU - Zhu, Zheng-Feng
AU  - Zhu ZF
AD  - Department of Cardiology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology Wuhan China.
FAU - Meng, Kai
AU  - Meng K
AD  - Department of Cardiology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology Wuhan China.
FAU - Peng, Yu-Dong
AU  - Peng YD
AD  - Department of Cardiology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology Wuhan China.
FAU - Sun, Hai-Tao
AU  - Sun HT
AD  - Department of Cardiology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology Wuhan China.
FAU - Wang, Yue
AU  - Wang Y
AD  - Department of Cardiology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology Wuhan China.
FAU - Pan, Cheng-Liang
AU  - Pan CL
AD  - Department of Cardiology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology Wuhan China.
FAU - Zeng, Qiu-Tang
AU  - Zeng QT
AD  - Department of Cardiology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology Wuhan China.
FAU - Yu, Kun-Wu
AU  - Yu KW
AD  - Department of Cardiology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology Wuhan China.
LA  - eng
PT  - Journal Article
DEP - 20210116
PL  - Japan
TA  - Circ Rep
JT  - Circulation reports
JID - 101746642
PMC - PMC7939956
OTO - NOTNLM
OT  - Atherosclerosis
OT  - Cellular immunity
OT  - Dendritic cells
OT  - Humoral immunity
OT  - Phosphorylcholine
COIS- None of the authors have any conflicts of interest to declare.
EDAT- 2021/03/12 06:00
MHDA- 2021/03/12 06:01
PMCR- 2021/01/16
CRDT- 2021/03/11 06:40
PHST- 2021/03/11 06:40 [entrez]
PHST- 2021/03/12 06:00 [pubmed]
PHST- 2021/03/12 06:01 [medline]
PHST- 2021/01/16 00:00 [pmc-release]
AID - 10.1253/circrep.CR-20-0118 [doi]
PST - epublish
SO  - Circ Rep. 2021 Jan 16;3(2):86-94. doi: 10.1253/circrep.CR-20-0118.