PMID- 33693566
OWN - NLM
STAT- MEDLINE
DCOM- 20210929
LR  - 20221207
IS  - 1460-2180 (Electronic)
IS  - 0143-3334 (Print)
IS  - 0143-3334 (Linking)
VI  - 42
IP  - 5
DP  - 2021 May 28
TI  - Ethnic differences in excretion of butadiene-DNA adducts by current smokers.
PG  - 694-704
LID - 10.1093/carcin/bgab020 [doi]
AB  - 1,3-Butadiene (BD) is a known human carcinogen used in the synthetic polymer 
      industry and also found in cigarette smoke, automobile exhaust and wood burning 
      smoke. BD is metabolically activated by cytochrome P450 monooxygenases (CYP) 2E1 
      and 2A6 to 3,4-epoxy-1-butene (EB), which can be detoxified by GST-catalyzed 
      glutathione conjugation or hydrolysis. We have previously observed ethnic 
      differences in urinary levels of EB-mercapturic acids in white, Japanese American 
      and Native Hawaiian smokers. In the present study, similar analyses were extended 
      to urinary BD-DNA adducts. BD-induced N7-(1-hydroxy-3-buten-2-yl) guanine 
      (EB-GII) adducts were quantified in urine samples obtained from smokers and 
      non-smokers belonging to three racial/ethnic groups: white, Japanese American and 
      Native Hawaiian. After adjusting for sex, age, nicotine equivalents, body mass 
      index and batch, we found that Japanese American smokers excreted significantly 
      higher amounts of urinary EB-GII than whites [1.45 (95% confidence interval: 
      1.12-1.87) versus 0.68 (95% confidence interval: 0.52-0.85) fmol/ml urine, P = 4 
      x 10-5]. Levels of urinary EB-GII in Native Hawaiian smokers were not different 
      from those in whites [0.67 (95% confidence interval: 0.51-0.84) fmol/ml urine, P 
      = 0.938]. There were no racial/ethnic differences in urinary EB-GII adduct levels 
      in non-smokers. Racial/ethnic differences in urinary EB-GII adduct levels in 
      smokers could not be explained by GSTT1 gene deletion or CYP2A6 enzymatic 
      activity. Urinary EB-GII adduct levels in smokers were significantly associated 
      with concentrations of BD metabolite dihyroxybutyl mercapturic acid. Overall, our 
      results reveal that urinary EB-GII adducts in smokers differ across racial/ethnic 
      groups. Future studies are required to understand genetic and epigenetic factors 
      that may be responsible for these differences.
CI  - (c) The Author(s) 2021. Published by Oxford University Press. All rights reserved. 
      For Permissions, please email: journals.permissions@oup.com.
FAU - Jokipii Krueger, Caitlin C
AU  - Jokipii Krueger CC
AD  - Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455, USA.
AD  - Department of Medicinal Chemistry, University of Minnesota, Minneapolis, MN 
      55455, USA.
FAU - Park, S Lani
AU  - Park SL
AD  - Epidemiology Program, University of Hawaii Cancer Center, Honolulu, HI 96822, 
      USA.
FAU - Madugundu, Guru
AU  - Madugundu G
AD  - Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455, USA.
AD  - Department of Medicinal Chemistry, University of Minnesota, Minneapolis, MN 
      55455, USA.
FAU - Patel, Yesha
AU  - Patel Y
AD  - Department of Preventative Medicine, School of Medicine, University of Southern 
      California, Los Angeles, CA 90089, USA.
FAU - Le Marchand, Loic
AU  - Le Marchand L
AD  - Epidemiology Program, University of Hawaii Cancer Center, Honolulu, HI 96822, 
      USA.
FAU - Stram, Daniel O
AU  - Stram DO
AD  - Department of Preventative Medicine, School of Medicine, University of Southern 
      California, Los Angeles, CA 90089, USA.
FAU - Tretyakova, Natalia
AU  - Tretyakova N
AUID- ORCID: 0000-0002-0621-6860
AD  - Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455, USA.
AD  - Department of Medicinal Chemistry, University of Minnesota, Minneapolis, MN 
      55455, USA.
LA  - eng
GR  - P01 CA138338/CA/NCI NIH HHS/United States
GR  - P30 CA077598/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - England
TA  - Carcinogenesis
JT  - Carcinogenesis
JID - 8008055
RN  - 0 (Butadienes)
RN  - 0 (Carcinogens)
RN  - 0 (DNA Adducts)
RN  - 0 (Epoxy Compounds)
RN  - 0 (Smoke)
RN  - 0 (Vehicle Emissions)
RN  - 478ERR5NKR (3,4-epoxy-1-butene)
RN  - EC 1.14.13.- (Cytochrome P-450 CYP2E1)
RN  - EC 1.14.14.1 (CYP2A6 protein, human)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP2A6)
RN  - EC 2.5.1.- (glutathione S-transferase T1)
RN  - EC 2.5.1.18 (Glutathione Transferase)
RN  - JSD5FGP5VD (1,3-butadiene)
RN  - WYQ7N0BPYC (Acetylcysteine)
SB  - IM
MH  - Acetylcysteine/urine
MH  - Adult
MH  - Aged
MH  - Asian/genetics
MH  - Butadienes/*toxicity
MH  - Carcinogens/metabolism/toxicity
MH  - Cytochrome P-450 CYP2A6/*genetics
MH  - Cytochrome P-450 CYP2E1/*genetics
MH  - DNA Adducts/*drug effects/genetics/urine
MH  - Epoxy Compounds/adverse effects/urine
MH  - Ethnicity/genetics
MH  - Female
MH  - Glutathione Transferase/genetics
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Native Hawaiian or Other Pacific Islander/genetics
MH  - Smoke/adverse effects
MH  - Smokers
MH  - Spectrometry, Mass, Electrospray Ionization
MH  - Tobacco Products/adverse effects
MH  - Vehicle Emissions/toxicity
MH  - White People/genetics
PMC - PMC8163050
EDAT- 2021/03/12 06:00
MHDA- 2021/09/30 06:00
PMCR- 2022/03/06
CRDT- 2021/03/11 06:47
PHST- 2020/11/06 00:00 [received]
PHST- 2021/02/10 00:00 [revised]
PHST- 2021/03/03 00:00 [accepted]
PHST- 2021/03/12 06:00 [pubmed]
PHST- 2021/09/30 06:00 [medline]
PHST- 2021/03/11 06:47 [entrez]
PHST- 2022/03/06 00:00 [pmc-release]
AID - 6159930 [pii]
AID - bgab020 [pii]
AID - 10.1093/carcin/bgab020 [doi]
PST - ppublish
SO  - Carcinogenesis. 2021 May 28;42(5):694-704. doi: 10.1093/carcin/bgab020.