PMID- 33694236
OWN - NLM
STAT- MEDLINE
DCOM- 20211015
LR  - 20221207
IS  - 1365-2672 (Electronic)
IS  - 1364-5072 (Linking)
VI  - 131
IP  - 4
DP  - 2021 Oct
TI  - Potential probiotics Lactobacillus casei K11 combined with plant extracts reduce 
      markers of type 2 diabetes mellitus in mice.
PG  - 1970-1982
LID - 10.1111/jam.15061 [doi]
AB  - AIMS: Probiotics and plant extracts have been used to prevent the development of 
      type 2 diabetes mellitus (T2DM). The study aimed to explore the effect of the 
      interaction between potential probiotics and bitter gourd extract (BGE) or 
      mulberry leaf extract (MLE) on T2DM. METHODS AND RESULTS: Potential probiotics 
      were tested for their gastrointestinal tract viability and growth situation 
      combined with BGE and MLE in vitro. The diabetes model was constructed in C57BL/6 
      mice, and the potential effect and mechanism of regulating blood glucose were 
      verified. Hematoxylin-eosin staining (HE), gas chromatography (GC), ELISA, and 
      RT-PCR were also used for analysis. The results showed that Lactobacillus casei 
      K11 had outstanding gastrointestinal tract viability and growth situation with 
      plant extracts. Administration of L. casei K11 combined with BGE and MLE 
      significantly reduced blood glucose levels and ameliorated insulin resistance in 
      diabetic mice than the administration of Lactobacillus paracasei J5 combined with 
      BGE and MLE. Moreover, in L. casei K11 combined with BGE and MLE groups, lipid 
      metabolism, oxidative stress, and proinflammatory cytokine levels were regulated. 
      Furthermore, the results indicated that L. casei K11 combined with BGE and MLE 
      improved free fatty acid receptor 2 (FFAR2) upregulation, glucagon-like peptide-1 
      (GLP-1) secretion, and short-chain fatty acid (SCFA) levels. CONCLUSIONS: These 
      findings showed that L. casei K11 combined with BGE and MLE modified the 
      SCFA-FFAR2-GLP-1 pathway to improve T2DM. SIGNIFICANCE AND IMPACT OF THE STUDY: 
      This study identified a new modality for evaluating interactions between 
      potential probiotics and plant extracts. Our findings revealed that L. casei K11 
      combined with BGE and MLE significantly promoted the SCFA-FFAR2-GLP-1 pathway to 
      inhibit T2DM.
CI  - (c) 2021 The Society for Applied Microbiology.
FAU - Zhang, Z
AU  - Zhang Z
AD  - College of Food Science and Engineering, Ocean University of China, Qingdao, 
      Shandong, China.
FAU - Bai, L
AU  - Bai L
AD  - College of Marine Life Sciences, Ocean University of China, Qingdao, Shandong, 
      China.
FAU - Guan, M
AU  - Guan M
AD  - Qingdao Central Hospital, Qingdao, Shandong, China.
FAU - Zhou, X
AU  - Zhou X
AD  - Qingdao Central Hospital, Qingdao, Shandong, China.
FAU - Liang, X
AU  - Liang X
AD  - College of Food Science and Engineering, Ocean University of China, Qingdao, 
      Shandong, China.
FAU - Lv, Y
AU  - Lv Y
AD  - College of Food Science and Engineering, Ocean University of China, Qingdao, 
      Shandong, China.
FAU - Yi, H
AU  - Yi H
AD  - College of Food Science and Engineering, Ocean University of China, Qingdao, 
      Shandong, China.
FAU - Zhou, H
AU  - Zhou H
AD  - College of Food Science and Engineering, Ocean University of China, Qingdao, 
      Shandong, China.
FAU - Liu, T
AU  - Liu T
AD  - College of Food Science and Engineering, Ocean University of China, Qingdao, 
      Shandong, China.
FAU - Gong, P
AU  - Gong P
AD  - College of Food Science and Engineering, Ocean University of China, Qingdao, 
      Shandong, China.
FAU - Sun, J
AU  - Sun J
AD  - Qingdao Central Hospital, Qingdao, Shandong, China.
FAU - Zhang, L
AU  - Zhang L
AUID- ORCID: 0000-0003-3459-2975
AD  - College of Food Science and Engineering, Ocean University of China, Qingdao, 
      Shandong, China.
LA  - eng
GR  - 2018YFC0311201/National Key R & D of China/
GR  - GA16B201-2/Heilongjiang province Key Sci. & Techn. Plan/
PT  - Journal Article
DEP - 20210408
PL  - England
TA  - J Appl Microbiol
JT  - Journal of applied microbiology
JID - 9706280
RN  - 0 (Plant Extracts)
SB  - IM
MH  - Animals
MH  - *Diabetes Mellitus, Experimental/drug therapy
MH  - *Diabetes Mellitus, Type 2/therapy
MH  - *Lacticaseibacillus casei
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Plant Extracts/pharmacology
MH  - *Probiotics
OTO - NOTNLM
OT  - bitter gourd extract
OT  - insulin resistance
OT  - mulberry leaf extract
OT  - probiotics
OT  - type 2 diabetes
EDAT- 2021/03/12 06:00
MHDA- 2021/10/16 06:00
CRDT- 2021/03/11 07:19
PHST- 2020/12/18 00:00 [revised]
PHST- 2020/10/21 00:00 [received]
PHST- 2021/03/08 00:00 [accepted]
PHST- 2021/03/12 06:00 [pubmed]
PHST- 2021/10/16 06:00 [medline]
PHST- 2021/03/11 07:19 [entrez]
AID - 10.1111/jam.15061 [doi]
PST - ppublish
SO  - J Appl Microbiol. 2021 Oct;131(4):1970-1982. doi: 10.1111/jam.15061. Epub 2021 
      Apr 8.