PMID- 33694264
OWN - NLM
STAT- MEDLINE
DCOM- 20210511
LR  - 20240401
IS  - 1365-2184 (Electronic)
IS  - 0960-7722 (Print)
IS  - 0960-7722 (Linking)
VI  - 54
IP  - 5
DP  - 2021 May
TI  - Tetrahedral DNA nanostructure improves transport efficiency and anti-fungal 
      effect of histatin 5 against Candida albicans.
PG  - e13020
LID - 10.1111/cpr.13020 [doi]
LID - e13020
AB  - OBJECTIVES: Anti-microbial peptides (AMPs) have been comprehensively investigated 
      as a novel alternative to traditional antibiotics against microorganisms. 
      Meanwhile, Tetrahedral DNA nanostructures (TDNs) have gained attention in the 
      field of biomedicine for their premium biological effects and transportation 
      efficiency as delivery vehicles. Hence, in this study, TDN/Histatin 5 (His-5) was 
      synthesized and the transport efficiency and anti-fungal effect were measured to 
      evaluate the promotion of His-5 modified by TDNs. MATERIALS AND METHODS: 
      Tetrahedral DNA nanostructures/His-5 complex was prepared via electrostatic 
      attraction and characterized by transmission electron microscopy (TEM), 
      polyacrylamide gel electrophoresis (PAGE), dynamic light scattering (DLS) and 
      electrophoretic light scattering (ELS). The anti-fungal effect of the TDN/His-5 
      complex was evaluated by determining the growth curve and colony-forming units of 
      C. albicans. The morphological transformation of C. albicans was observed by 
      light microscope and scanning electron microscope (SEM). Immunofluorescence was 
      performed, and potassium efflux was detected to mechanistically demonstrate the 
      efficacy of TDN/His-5. RESULTS: The results showed that Histatin 5 modified by 
      TDNs had preferable stability in serum and was effectively transported into 
      C. albicans, leading to the increased formation of intracellular reactive oxygen 
      species, higher potassium efflux and enhanced anti-fungal effect against 
      C. albicans. CONCLUSIONS: Our study showed that TDN/His-5 was synthesized 
      successfully. And by the modification of TDNs, His-5 showed increased transport 
      efficiency and improved anti-fungal effect.
CI  - (c) 2021 The Authors. Cell Proliferation Published by John Wiley & Sons Ltd.
FAU - Zhang, Bowen
AU  - Zhang B
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral 
      Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
FAU - Qin, Xin
AU  - Qin X
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral 
      Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
FAU - Zhou, Mi
AU  - Zhou M
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral 
      Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
FAU - Tian, Taoran
AU  - Tian T
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral 
      Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
FAU - Sun, Yue
AU  - Sun Y
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral 
      Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
FAU - Li, Songhang
AU  - Li S
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral 
      Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
FAU - Xiao, Dexuan
AU  - Xiao D
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral 
      Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
FAU - Cai, Xiaoxiao
AU  - Cai X
AUID- ORCID: 0000-0002-5654-7414
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral 
      Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
LA  - eng
GR  - 2019YFA0110600/National Key R&D Program of China/
GR  - 81970986/National Nature Science Foundation of China/
GR  - 81771125/National Nature Science Foundation of China/
PT  - Journal Article
DEP - 20210311
PL  - England
TA  - Cell Prolif
JT  - Cell proliferation
JID - 9105195
RN  - 0 (Antifungal Agents)
RN  - 0 (Histatins)
RN  - 0 (Reactive Oxygen Species)
RN  - 9007-49-2 (DNA)
RN  - RWP5GA015D (Potassium)
SB  - IM
MH  - Antifungal Agents/chemistry/metabolism/*pharmacology
MH  - Candida albicans/*drug effects
MH  - DNA/*chemistry
MH  - Drug Stability
MH  - Histatins/*chemistry
MH  - Nanostructures/*chemistry/toxicity
MH  - Potassium/metabolism
MH  - Reactive Oxygen Species/metabolism
MH  - Static Electricity
PMC - PMC8088467
OTO - NOTNLM
OT  - Candida albicans
OT  - Fungi
OT  - Histatin 5
OT  - Tetrahedral framework nucleic acids
OT  - anti-microbial peptides
COIS- There are no conflicts to declare.
EDAT- 2021/03/12 06:00
MHDA- 2021/05/12 06:00
PMCR- 2021/03/11
CRDT- 2021/03/11 07:21
PHST- 2021/02/03 00:00 [revised]
PHST- 2020/12/26 00:00 [received]
PHST- 2021/02/20 00:00 [accepted]
PHST- 2021/03/12 06:00 [pubmed]
PHST- 2021/05/12 06:00 [medline]
PHST- 2021/03/11 07:21 [entrez]
PHST- 2021/03/11 00:00 [pmc-release]
AID - CPR13020 [pii]
AID - 10.1111/cpr.13020 [doi]
PST - ppublish
SO  - Cell Prolif. 2021 May;54(5):e13020. doi: 10.1111/cpr.13020. Epub 2021 Mar 11.