PMID- 33712069
OWN - NLM
STAT- MEDLINE
DCOM- 20210708
LR  - 20240331
IS  - 1757-6512 (Electronic)
IS  - 1757-6512 (Linking)
VI  - 12
IP  - 1
DP  - 2021 Mar 12
TI  - Morusin induces osteogenic differentiation of bone marrow mesenchymal stem cells 
      by canonical Wnt/beta-catenin pathway and prevents bone loss in an ovariectomized 
      rat model.
PG  - 173
LID - 10.1186/s13287-021-02239-3 [doi]
LID - 173
AB  - BACKGROUND: Osteoporosis (OP) is a metabolic bone disease due to the imbalance of 
      osteogenesis and bone resorption, in which, bone marrow mesenchymal stem cells 
      (BMSCs) have a significant effect as the seed cells. Recent research has shown 
      the function of Morusin on inhibiting osteoclast differentiation in vitro. 
      However, whether Morusin can regulate the osteogenic differentiation in addition 
      to the proliferation of BMSCs remains unclear. METHODS: BMSCs were isolated from 
      4-week-old Wistar rats and then treated with different concentrations of Morusin 
      for 3, 5, 7, and 14 days. The proliferation of BMSCs was detected by MTT assay. 
      The effect of Morusin on osteogenic differentiation of BMSCs was detected by 
      RT-qPCR, Western blotting, ALP, and Alizarin Red staining. The effect of Morusin 
      on Wnt/beta-catenin signaling pathway was analyzed by RT-qPCR, Western blotting, and 
      immunofluorescence. Finally, in the ovariectomy-induced osteoporosis model, the 
      anti-osteoporosis activity of Morusin was determined by micro-CT, HE, and 
      immunohistochemistry. RESULTS: The results showed the function of 2.5-10 muM 
      Morusin in the promotion of the proliferation in addition to osteogenic 
      differentiation of BMSCs. Moreover, it also has an impact in activating the 
      Wnt/beta-catenin signaling pathway via inhibition of beta-catenin phosphorylation as 
      well as promotion of its nuclear translocation. Upon Dickkopf-related protein-1 
      (DKK-1, an inhibitor of the Wnt/beta-catenin signaling pathway) was added to the 
      Morusin, Morusin had a decreased stimulatory osteogenic effect on BMSCs. Finally, 
      in the rat OP model, we found that Morusin could also exert anti-osteoporosis 
      activity in vivo. CONCLUSIONS: This study indicates the ability of Morusin in the 
      promotion of osteogenic differentiation of BMSCs via the activation of 
      Wnt/beta-catenin signaling pathway and also shows the potential of Morusin to be an 
      agent for osteoporosis treatment.
FAU - Chen, Ming
AU  - Chen M
AD  - Department of Joint Surgery and Sports medicine, Zhongnan Hospital of Wuhan 
      University, Wuhan, 430071, China.
AD  - Hubei Provincial Key Laboratory of Developmentally Originated Disease, Wuhan, 
      430071, China.
FAU - Han, Hui
AU  - Han H
AD  - Department of Joint Surgery and Sports medicine, Zhongnan Hospital of Wuhan 
      University, Wuhan, 430071, China.
AD  - Hubei Provincial Key Laboratory of Developmentally Originated Disease, Wuhan, 
      430071, China.
FAU - Zhou, Siqi
AU  - Zhou S
AD  - Department of Joint Surgery and Sports medicine, Zhongnan Hospital of Wuhan 
      University, Wuhan, 430071, China.
AD  - Hubei Provincial Key Laboratory of Developmentally Originated Disease, Wuhan, 
      430071, China.
AD  - Department of Orthopedics Department, Renmin Hospital of Wuhan University, Wuhan, 
      430060, China.
FAU - Wen, Yinxian
AU  - Wen Y
AD  - Department of Joint Surgery and Sports medicine, Zhongnan Hospital of Wuhan 
      University, Wuhan, 430071, China. wenyinxian@whu.edu.cn.
AD  - Hubei Provincial Key Laboratory of Developmentally Originated Disease, Wuhan, 
      430071, China. wenyinxian@whu.edu.cn.
FAU - Chen, Liaobin
AU  - Chen L
AD  - Department of Joint Surgery and Sports medicine, Zhongnan Hospital of Wuhan 
      University, Wuhan, 430071, China. lbchen@whu.edu.cn.
AD  - Hubei Provincial Key Laboratory of Developmentally Originated Disease, Wuhan, 
      430071, China. lbchen@whu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210312
PL  - England
TA  - Stem Cell Res Ther
JT  - Stem cell research & therapy
JID - 101527581
RN  - 0 (Flavonoids)
RN  - 0 (beta Catenin)
RN  - 62596-29-6 (morusin)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/metabolism
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Female
MH  - Flavonoids
MH  - *Mesenchymal Stem Cells/metabolism
MH  - Osteogenesis
MH  - *Osteoporosis
MH  - Rats
MH  - Rats, Wistar
MH  - Wnt Signaling Pathway
MH  - beta Catenin/genetics/metabolism
PMC - PMC7953707
OTO - NOTNLM
OT  - BMSCs
OT  - Morusin
OT  - Osteoporosis
OT  - Wnt/beta-catenin
COIS- The authors declare that they have no competing interests.
EDAT- 2021/03/14 06:00
MHDA- 2021/07/09 06:00
PMCR- 2021/03/12
CRDT- 2021/03/13 05:32
PHST- 2021/01/22 00:00 [received]
PHST- 2021/02/24 00:00 [accepted]
PHST- 2021/03/13 05:32 [entrez]
PHST- 2021/03/14 06:00 [pubmed]
PHST- 2021/07/09 06:00 [medline]
PHST- 2021/03/12 00:00 [pmc-release]
AID - 10.1186/s13287-021-02239-3 [pii]
AID - 2239 [pii]
AID - 10.1186/s13287-021-02239-3 [doi]
PST - epublish
SO  - Stem Cell Res Ther. 2021 Mar 12;12(1):173. doi: 10.1186/s13287-021-02239-3.