PMID- 33712144
OWN - NLM
STAT- MEDLINE
DCOM- 20210407
LR  - 20210407
IS  - 1879-1344 (Electronic)
IS  - 0144-8617 (Linking)
VI  - 260
DP  - 2021 May 15
TI  - Characterization, antioxidant and immunomodulatory effects of selenized 
      polysaccharides from dandelion roots.
PG  - 117796
LID - S0144-8617(21)00183-1 [pii]
LID - 10.1016/j.carbpol.2021.117796 [doi]
AB  - The polysaccharide (DRP) was gained from dandelion roots by ultrasonic-assisted 
      enzymatic extraction (UAEE) followed by two-step column purification. Then 
      selenylation of DRP has been accomplished by HNO(3)-Na(2)SeO(3) method. sDRP-1 
      and sDRP-2 with the selenium content of 170 +/- 1.13 and 710 +/- 4.00 mug/g were 
      prepared for further structural characterization and bioactivity determination. 
      DRP, sDRP-1, and sDRP-2 were composed of the same monosaccharides in different 
      molar ratios, and the molecular weights of DRP, sDRP-1 and sDRP-2 were 8700, 
      7900, and 5600 Da, respectively. Fourier transform infrared (FT-IR) spectra 
      confirmed that DRP, sDRP-1, and sDRP-2 possessed similar functional groups. The 
      results of Congo red test, X-ray diffraction (XRD) and scanning electron 
      microscopy (SEM) showed that DRP, sDRP-1, and sDRP-2 had no three helix 
      structure, did not form single crystal, and all belonged to amorphous morphology. 
      sDRP-1 and sDRP-2 possessed greater antioxidant activities in vitro than the 
      native polysaccharide DRP. At the same time, the selenized polysaccharides showed 
      better immunomodulatory ability and could be used as new-type immunoenhancer. The 
      present conclusions provided theoretical basis for the new application of 
      dandelion polysaccharides and the development of dandelion resources.
CI  - Copyright (c) 2021. Published by Elsevier Ltd.
FAU - Wang, Libo
AU  - Wang L
AD  - College of Art and Science, Northeast Agricultural University, Harbin 150030, 
      China.
FAU - Li, Lianyu
AU  - Li L
AD  - College of Art and Science, Northeast Agricultural University, Harbin 150030, 
      China.
FAU - Gao, Jingyu
AU  - Gao J
AD  - College of Art and Science, Northeast Agricultural University, Harbin 150030, 
      China.
FAU - Huang, Jing
AU  - Huang J
AD  - College of Pharmacy, Harbin Medical University, Harbin 150030, China.
FAU - Yang, Yu
AU  - Yang Y
AD  - College of Art and Science, Northeast Agricultural University, Harbin 150030, 
      China.
FAU - Xu, Yaqin
AU  - Xu Y
AD  - College of Art and Science, Northeast Agricultural University, Harbin 150030, 
      China. Electronic address: xuyaqin@neau.edu.cn.
FAU - Liu, Shuang
AU  - Liu S
AD  - College of Art and Science, Northeast Agricultural University, Harbin 150030, 
      China.
FAU - Yu, Wenqing
AU  - Yu W
AD  - College of Art and Science, Northeast Agricultural University, Harbin 150030, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20210213
PL  - England
TA  - Carbohydr Polym
JT  - Carbohydrate polymers
JID - 8307156
RN  - 0 (Antioxidants)
RN  - 0 (Immunologic Factors)
RN  - 0 (Polysaccharides)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - H6241UJ22B (Selenium)
SB  - IM
MH  - Animals
MH  - Antioxidants/*chemistry
MH  - Cell Survival/drug effects
MH  - Hydrogen Peroxide/pharmacology
MH  - Immunologic Factors/chemistry/metabolism/pharmacology
MH  - Macrophages/cytology/drug effects/metabolism
MH  - Mice
MH  - Molecular Weight
MH  - Phagocytosis/drug effects
MH  - Plant Roots/metabolism
MH  - Polysaccharides/*chemistry/metabolism/pharmacology
MH  - RAW 264.7 Cells
MH  - Selenium/*chemistry
MH  - Taraxacum/*metabolism
OTO - NOTNLM
OT  - Ability
OT  - Antioxidant activity
OT  - Dandelion
OT  - Immunomodulatory
OT  - Polysaccharides
OT  - Selenylation
EDAT- 2021/03/14 06:00
MHDA- 2021/04/10 06:00
CRDT- 2021/03/13 05:37
PHST- 2020/11/13 00:00 [received]
PHST- 2021/01/27 00:00 [revised]
PHST- 2021/02/06 00:00 [accepted]
PHST- 2021/03/13 05:37 [entrez]
PHST- 2021/03/14 06:00 [pubmed]
PHST- 2021/04/10 06:00 [medline]
AID - S0144-8617(21)00183-1 [pii]
AID - 10.1016/j.carbpol.2021.117796 [doi]
PST - ppublish
SO  - Carbohydr Polym. 2021 May 15;260:117796. doi: 10.1016/j.carbpol.2021.117796. Epub 
      2021 Feb 13.