PMID- 33714729
OWN - NLM
STAT- MEDLINE
DCOM- 20210910
LR  - 20220503
IS  - 1090-2163 (Electronic)
IS  - 0008-8749 (Print)
IS  - 0008-8749 (Linking)
VI  - 363
DP  - 2021 May
TI  - Human splenic myeloid derived suppressor cells: Phenotypic and clustering 
      analysis.
PG  - 104317
LID - S0008-8749(21)00036-8 [pii]
LID - 10.1016/j.cellimm.2021.104317 [doi]
AB  - Myeloid derived suppressor cells (MDSCs) can be subset into monocytic (M-), 
      granulocytic (G-) or polymorphonuclear (PMN-), and immature (i-) or early MDSCs 
      and have a role in many disease states. In cancer patients, the frequencies of 
      MDSCs can positively correlate with stage, grade, and survival. Most clinical 
      studies into MDSCs have been undertaken with peripheral blood (PB); however, in 
      the present studies, we uniquely examined MDSCs in the spleens and PB from 
      patients with gastrointestinal cancers. In our studies, MDSCs were rigorously 
      subset using the following markers: Lineage (LIN) (CD3, CD19 and CD56), human 
      leukocyte antigen (HLA)-DR, CD11b, CD14, CD15, CD33, CD34, CD45, and CD16. We 
      observed a significantly higher frequency of PMN- and M-MDSCs in the PB of cancer 
      patients as compared to their spleens. Expression of the T-cell suppressive 
      enzymes arginase (ARG1) and inducible nitric oxide synthase (i-NOS) were higher 
      on all MDSC subsets for both cancer patients PB and spleen cells as compared to 
      MDSCs from the PB of normal donors. Similar findings for the activation markers 
      lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1), program death 
      ligand 1 (PD-L1) and program cell death protein 1 (PD-1) were observed. 
      Interestingly, the total MDSC cell number exported to clustering analyses was 
      similar between all sample types; however, clustering analyses of these MDSCs, 
      using these markers, uniquely documented novel subsets of PMN-, M- and i-MDSCs. 
      In summary, we report a comparison of splenic MDSC frequency, subtypes, and 
      functionality in cancer patients to their PB by clustering and cytometric 
      analyses.
CI  - Copyright (c) 2021 Elsevier Inc. All rights reserved.
FAU - Cole, Kathryn E
AU  - Cole KE
AD  - Department of Pathology and Microbiology, University of Nebraska Medical Center, 
      Omaha, NE 68198, United States.
FAU - Ly, Quan P
AU  - Ly QP
AD  - Department of Surgery, University of Nebraska Medical Center, Omaha, NE 
      68198-4990, United States.
FAU - Hollingsworth, Michael A
AU  - Hollingsworth MA
AD  - Eppley Institute for Research in Cancer and Allied Diseases, University of 
      Nebraska Medical Center, Omaha, NE 68198-5950, United States.
FAU - Cox, Jesse L
AU  - Cox JL
AD  - Department of Pathology and Microbiology, University of Nebraska Medical Center, 
      Omaha, NE 68198, United States.
FAU - Padussis, James C
AU  - Padussis JC
AD  - Department of Surgery, University of Nebraska Medical Center, Omaha, NE 
      68198-4990, United States.
FAU - Foster, Jason M
AU  - Foster JM
AD  - Department of Surgery, University of Nebraska Medical Center, Omaha, NE 
      68198-4990, United States.
FAU - Vargas, Luciano M
AU  - Vargas LM
AD  - Department of Surgery, University of Nebraska Medical Center, Omaha, NE 
      68198-4990, United States.
FAU - Talmadge, James E
AU  - Talmadge JE
AD  - Department of Pathology and Microbiology, University of Nebraska Medical Center, 
      Omaha, NE 68198, United States; Department of Internal Medicine, University of 
      Nebraska Medical Center, Omaha, NE 68198, United States. Electronic address: 
      jtalmadg@unmc.edu.
LA  - eng
GR  - P30 CA036727/CA/NCI NIH HHS/United States
GR  - P50 CA127297/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20210301
PL  - Netherlands
TA  - Cell Immunol
JT  - Cellular immunology
JID - 1246405
RN  - 0 (B7-H1 Antigen)
RN  - 0 (CD274 protein, human)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (OLR1 protein, human)
RN  - 0 (PDCD1 protein, human)
RN  - 0 (Programmed Cell Death 1 Receptor)
RN  - 0 (Scavenger Receptors, Class E)
RN  - EC 3.5.3.1 (Arginase)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Arginase/metabolism
MH  - B7-H1 Antigen/metabolism
MH  - CD4-Positive T-Lymphocytes/immunology
MH  - CD8-Positive T-Lymphocytes/immunology
MH  - Cluster Analysis
MH  - Female
MH  - Flow Cytometry/methods
MH  - Gastrointestinal Neoplasms/immunology
MH  - HLA-DR Antigens/metabolism
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Myeloid-Derived Suppressor Cells/cytology/immunology/*metabolism
MH  - Neoplasms/immunology
MH  - Programmed Cell Death 1 Receptor/metabolism
MH  - Scavenger Receptors, Class E/metabolism
MH  - Spleen/*immunology/pathology
PMC - PMC8077751
MID - NIHMS1683116
OTO - NOTNLM
OT  - Cancer patient peripheral blood
OT  - Cancer patient spleen
OT  - Flow cytometry
OT  - MDSC
OT  - SPADE
EDAT- 2021/03/15 06:00
MHDA- 2021/09/11 06:00
PMCR- 2022/05/01
CRDT- 2021/03/14 20:41
PHST- 2020/11/17 00:00 [received]
PHST- 2021/02/11 00:00 [revised]
PHST- 2021/02/12 00:00 [accepted]
PHST- 2021/03/15 06:00 [pubmed]
PHST- 2021/09/11 06:00 [medline]
PHST- 2021/03/14 20:41 [entrez]
PHST- 2022/05/01 00:00 [pmc-release]
AID - S0008-8749(21)00036-8 [pii]
AID - 10.1016/j.cellimm.2021.104317 [doi]
PST - ppublish
SO  - Cell Immunol. 2021 May;363:104317. doi: 10.1016/j.cellimm.2021.104317. Epub 2021 
      Mar 1.