PMID- 33719421
OWN - NLM
STAT- MEDLINE
DCOM- 20210514
LR  - 20221207
IS  - 1520-5118 (Electronic)
IS  - 0021-8561 (Print)
IS  - 0021-8561 (Linking)
VI  - 69
IP  - 11
DP  - 2021 Mar 24
TI  - IgE-Reactivity Pattern of Tomato Seed and Peel Nonspecific Lipid-Transfer 
      Proteins after in Vitro Gastrointestinal Digestion.
PG  - 3511-3518
LID - 10.1021/acs.jafc.0c06949 [doi]
AB  - The influence of gastrointestinal digestion on the immunological properties of 
      three different nonspecific lipid-transfer proteins (nsLTPs) described in tomato 
      fruit has been assessed using an in vitro system mimicking the stomach and 
      intestine digestion conditions. Tomato peel/pulp nsLTP, Sola l 3, was degraded 
      after digestion, although the immunoglobulin E (IgE) recognition of intact 
      protein and a 10 kDa band were still observed after 30 min of duodenal digestion 
      in the presence of phosphatidylcholine. The tomato seed nsLTP, Sola l 7, showed a 
      higher stability than the other seed allergen, Sola l 6, during digestion. Sola l 
      7 showed an IgE immunoreactive 6.5 kDa band in immunoblotting analysis, retaining 
      up to 7% of its IgE-binding capacity in inhibition ELISA test after 60 min of 
      duodenal digestion and keeping intact its ability to activate basophils after 
      digestion. These results suggest that the tomato seed allergen Sola l 7 might be 
      considered as an important allergen in the induction of allergic responses to 
      tomato due to its high stability against gastrointestinal digestion.
FAU - Martin-Pedraza, Laura
AU  - Martin-Pedraza L
AD  - Department of Biochemistry and Molecular Biology, Universidad Complutense de 
      Madrid, 28040 Madrid, Spain.
FAU - Mayorga, Cristobalina
AU  - Mayorga C
AD  - Allergy Research Laboratory, IBIMA, Regional University Hospital of Malaga, UMA, 
      29009 Malaga, Spain.
FAU - Gomez, Francisca
AU  - Gomez F
AD  - Allergy Research Laboratory, IBIMA, Regional University Hospital of Malaga, UMA, 
      29009 Malaga, Spain.
FAU - Bueno-Diaz, Cristina
AU  - Bueno-Diaz C
AD  - Department of Biochemistry and Molecular Biology, Universidad Complutense de 
      Madrid, 28040 Madrid, Spain.
FAU - Blanca-Lopez, Natalia
AU  - Blanca-Lopez N
AD  - Servicio de Alergia, Hospital Universitario Infanta Leonor, 28031 Madrid, Spain.
FAU - Gonzalez, Miguel
AU  - Gonzalez M
AD  - Allergy Research Laboratory, IBIMA, Regional University Hospital of Malaga, UMA, 
      29009 Malaga, Spain.
FAU - Martinez-Blanco, Monica
AU  - Martinez-Blanco M
AD  - Instituto de Investigacion en Ciencias de la Alimentacion (CIAL, CSIC-UAM), 
      Nicolas Cabrera 9, 28049 Madrid, Spain.
FAU - Cuesta-Herranz, Javier
AU  - Cuesta-Herranz J
AD  - Fundacion IIS-Fundacion Jimenez Diaz, 28040 Madrid, Spain.
FAU - Molina, Elena
AU  - Molina E
AUID- ORCID: 0000-0001-7918-7768
AD  - Instituto de Investigacion en Ciencias de la Alimentacion (CIAL, CSIC-UAM), 
      Nicolas Cabrera 9, 28049 Madrid, Spain.
FAU - Villalba, Mayte
AU  - Villalba M
AD  - Department of Biochemistry and Molecular Biology, Universidad Complutense de 
      Madrid, 28040 Madrid, Spain.
FAU - Benede, Sara
AU  - Benede S
AUID- ORCID: 0000-0002-9288-9438
AD  - Instituto de Investigacion en Ciencias de la Alimentacion (CIAL, CSIC-UAM), 
      Nicolas Cabrera 9, 28049 Madrid, Spain.
LA  - eng
PT  - Journal Article
DEP - 20210315
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (Allergens)
RN  - 0 (Antigens, Plant)
RN  - 0 (Lipids)
RN  - 0 (Plant Proteins)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Allergens
MH  - Antigens, Plant
MH  - Digestion
MH  - *Food Hypersensitivity
MH  - Immunoglobulin E
MH  - Lipids
MH  - *Solanum lycopersicum
MH  - Plant Proteins
MH  - Seeds
PMC - PMC9134490
OTO - NOTNLM
OT  - IgE binding
OT  - anaphylaxis
OT  - gastrointestinal digestion
OT  - nsLTP
OT  - tomato allergy
COIS- The authors declare no competing financial interest.
EDAT- 2021/03/16 06:00
MHDA- 2021/05/15 06:00
PMCR- 2022/05/26
CRDT- 2021/03/15 12:26
PHST- 2021/03/16 06:00 [pubmed]
PHST- 2021/05/15 06:00 [medline]
PHST- 2021/03/15 12:26 [entrez]
PHST- 2022/05/26 00:00 [pmc-release]
AID - 10.1021/acs.jafc.0c06949 [doi]
PST - ppublish
SO  - J Agric Food Chem. 2021 Mar 24;69(11):3511-3518. doi: 10.1021/acs.jafc.0c06949. 
      Epub 2021 Mar 15.