PMID- 33722663
OWN - NLM
STAT- MEDLINE
DCOM- 20220113
LR  - 20220113
IS  - 1096-1194 (Electronic)
IS  - 0890-8508 (Linking)
VI  - 57
DP  - 2021 Jun
TI  - Overexpression of long non-coding RNA XIST promotes IL-1beta-induced degeneration of 
      nucleus pulposus cells through targeting miR-499a-5p.
PG  - 101711
LID - S0890-8508(21)00018-9 [pii]
LID - 10.1016/j.mcp.2021.101711 [doi]
AB  - BACKGROUND: Long non-coding RNA X-interactive specific transcript (XIST) is 
      implicated in many diseases. However, its role and interaction with microRNA 
      (miR)-499a-5p in intervertebral disc degeneration (IDD) remained unclear. 
      METHODS: Nucleus pulposus (NP) tissue samples were collected and nucleus pulposus 
      cells (NPCs) were isolated for Interleukin-1beta (IL-1beta) treatment and 
      identification. XIST and miR-499a-5p expressions in the tissue were measured with 
      quantitative real-time polymerase chain reaction (qRT-PCR). After IL-1beta 
      treatment, NPC apoptosis was detected by flow cytometry. The potential binding 
      sites of XIST and miR-499a-5p were predicted by starBase and confirmed by 
      dual-luciferase reporter assay. Relative expressions of tissue inhibitor of 
      metalloproteinases-3 (TIMP-3), Matrix metalloproteinases-3 (MMP-3), MMP-13, 
      Collagen II, Aggrecan and apoptosis-related proteins (Bcl-2 associated X protein, 
      Bax; B-cell lymphoma 2, Bcl-2; cleaved caspase-3) were measured by qRT-PCR and 
      Western blot as needed. RESULTS: XIST expression was upregulated in the NP 
      tissues of patients with IDD, and IL-1beta treatment resulted in a degradation of 
      NPCs. Overexpressed XIST promoted the effects of IL-1beta on increasing NPC 
      apoptosis and expressions of XIST, MMP-3, MMP-13, Bax and Cleaved caspase-3, but 
      down-regulated TIMP-3, Collagen II, Aggrecan and Bcl-2 expressions. Silencing 
      XIST, however, showed the opposite effects to its overexpression. MiR-499a-5p 
      expression was downregulated in NP tissues of IDD patients and could bind with 
      XIST, while its upregulation reversed the effects of overexpressed XIST in the 
      IL-1beta-treated NPCs. CONCLUSION: Overexpressed XIST caused NPC degeneration 
      through promoting apoptosis and extracellular matrix degradation of IL-1beta-treated 
      NPCs through targeting miR-499a-5p, and therefore can serve as a potential 
      treatment for IDD.
CI  - Copyright (c) 2021 Elsevier Ltd. All rights reserved.
FAU - He, Jun
AU  - He J
AD  - Department of Orthopedics, Zhejiang Hospital, Xihu District, Hangzhou City, 
      Zhejiang Province, 310030, China.
FAU - Yang, Jing
AU  - Yang J
AD  - Department of Cardiology, Zhejiang Hospital, Xihu District, Hangzhou, Zhejiang, 
      310013, China.
FAU - Shen, Tulan
AU  - Shen T
AD  - Outpatient Department, Zhejiang Hospital, Xihu District, Hangzhou City, Zhejiang 
      Province, 310030, China.
FAU - He, Jian
AU  - He J
AD  - Department of Orthopedics, Zhejiang Hospital, Xihu District, Hangzhou City, 
      Zhejiang Province, 310030, China. Electronic address: hejian_jhe@163.com.
LA  - eng
PT  - Journal Article
DEP - 20210317
PL  - England
TA  - Mol Cell Probes
JT  - Molecular and cellular probes
JID - 8709751
RN  - 0 (Interleukin-1beta)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Long Noncoding)
SB  - IM
MH  - Apoptosis/genetics
MH  - Cells, Cultured
MH  - Extracellular Matrix
MH  - Humans
MH  - Interleukin-1beta/genetics
MH  - *MicroRNAs/genetics
MH  - *Nucleus Pulposus
MH  - *RNA, Long Noncoding/genetics
OTO - NOTNLM
OT  - Extracellular matrix degradation
OT  - Intervertebral disc degeneration
OT  - Nucleus pulposus cells
OT  - X-interactive specific transcript
OT  - microRNA-499a-5p
EDAT- 2021/03/17 06:00
MHDA- 2022/01/14 06:00
CRDT- 2021/03/16 06:09
PHST- 2020/10/08 00:00 [received]
PHST- 2021/02/07 00:00 [revised]
PHST- 2021/03/08 00:00 [accepted]
PHST- 2021/03/17 06:00 [pubmed]
PHST- 2022/01/14 06:00 [medline]
PHST- 2021/03/16 06:09 [entrez]
AID - S0890-8508(21)00018-9 [pii]
AID - 10.1016/j.mcp.2021.101711 [doi]
PST - ppublish
SO  - Mol Cell Probes. 2021 Jun;57:101711. doi: 10.1016/j.mcp.2021.101711. Epub 2021 
      Mar 17.