PMID- 33725198
OWN - NLM
STAT- MEDLINE
DCOM- 20210901
LR  - 20210901
IS  - 1432-119X (Electronic)
IS  - 0948-6143 (Linking)
VI  - 156
IP  - 1
DP  - 2021 Jul
TI  - Relationship between serum concentration, functional parameters and cell 
      bioenergetics in IPEC-J2 cell line.
PG  - 59-67
LID - 10.1007/s00418-021-01981-2 [doi]
AB  - The foetal bovine serum (FBS) concentration could influence functional parameters 
      of IPEC-J2 cells. IPEC-J2 is a non-transformed continuous epithelial cell line 
      that represents an established in vitro model to study porcine gut inflammation 
      and alterations of intestinal integrity. This cell line also represents a good 
      translational model thanks to the high similitudes between pig and human 
      gastrointestinal tract. With the aim to assess if the FBS-dependent functional 
      variations are linked to the bioenergetic aspects, the addition of 5% and 10% FBS 
      in the IPEC-J2 culture medium were tested. Doubling time and TEER measurement 
      indicated that cells cultured at higher FBS dose grow faster and as a more 
      compact monolayer. 10% FBS increases ATP production and mitochondrial oxidative 
      phosphorylation (OxPhos) and does not affect glycolysis. Both at 5% and 10% FBS 
      ATP production mainly comes from OxPhos and FBS concentration does not affect the 
      cell respiration bioenergetic parameters. Noteworthy, IPEC-J2 treated with 5% and 
      10% FBS have a metabolic potential since both OxPhos and glycolysis increase 
      by > 100% and < 50%, respectively in comparison with baseline metabolism. 
      Moreover, glucose, fatty acids and glutamine constitute the preferred metabolic 
      fuel for mitochondrial respiration at both FBS conditions tested. Accordingly, 
      the cells flexibility to oxidize these substrates shows that IPEC-J2 mitochondria 
      cannot maintain the basal ATP production without oxidizing all the substrates 
      available irrespective of FBS concentration. To sum up, in IPEC-J2 cells OxPhos 
      increases with the FBS-stimulated functional physiological parameters to fulfil 
      ATP requirements.
CI  - (c) 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, 
      part of Springer Nature.
FAU - Bernardini, Chiara
AU  - Bernardini C
AD  - Department of Veterinary Medical Science, University of Bologna, Via Tolara di 
      Sopra, 50, 40064, Ozzano Emilia, BO, Italy.
FAU - Algieri, Cristina
AU  - Algieri C
AD  - Department of Veterinary Medical Science, University of Bologna, Via Tolara di 
      Sopra, 50, 40064, Ozzano Emilia, BO, Italy.
FAU - Mantia, La Debora
AU  - Mantia D
AD  - Department of Veterinary Medical Science, University of Bologna, Via Tolara di 
      Sopra, 50, 40064, Ozzano Emilia, BO, Italy.
FAU - Zannoni, Augusta
AU  - Zannoni A
AD  - Department of Veterinary Medical Science, University of Bologna, Via Tolara di 
      Sopra, 50, 40064, Ozzano Emilia, BO, Italy.
AD  - Health Sciences and Technologies-Interdepartmental Center for Industrial Research 
      (CIRI-SDV), Alma Mater Studiorum, University of Bologna, Bologna, Italy.
FAU - Salaroli, Roberta
AU  - Salaroli R
AD  - Department of Veterinary Medical Science, University of Bologna, Via Tolara di 
      Sopra, 50, 40064, Ozzano Emilia, BO, Italy.
FAU - Trombetti, Fabiana
AU  - Trombetti F
AD  - Department of Veterinary Medical Science, University of Bologna, Via Tolara di 
      Sopra, 50, 40064, Ozzano Emilia, BO, Italy.
FAU - Forni, Monica
AU  - Forni M
AD  - Department of Veterinary Medical Science, University of Bologna, Via Tolara di 
      Sopra, 50, 40064, Ozzano Emilia, BO, Italy. monica.forni@unibo.it.
AD  - Health Sciences and Technologies-Interdepartmental Center for Industrial Research 
      (CIRI-SDV), Alma Mater Studiorum, University of Bologna, Bologna, Italy. 
      monica.forni@unibo.it.
FAU - Pagliarani, Alessandra
AU  - Pagliarani A
AD  - Department of Veterinary Medical Science, University of Bologna, Via Tolara di 
      Sopra, 50, 40064, Ozzano Emilia, BO, Italy.
FAU - Nesci, Salvatore
AU  - Nesci S
AUID- ORCID: 0000-0001-8569-7158
AD  - Department of Veterinary Medical Science, University of Bologna, Via Tolara di 
      Sopra, 50, 40064, Ozzano Emilia, BO, Italy.
LA  - eng
GR  - 2019.0534/CARISBO Foundation/
PT  - Journal Article
DEP - 20210316
PL  - Germany
TA  - Histochem Cell Biol
JT  - Histochemistry and cell biology
JID - 9506663
RN  - 8L70Q75FXE (Adenosine Triphosphate)
SB  - IM
MH  - Adenosine Triphosphate/*biosynthesis/blood
MH  - Animals
MH  - Cattle
MH  - Cells, Cultured
MH  - Fetal Blood/*metabolism
MH  - Swine
OTO - NOTNLM
OT  - Cell metabolism
OT  - Foetal bovine serum
OT  - Glycolysis
OT  - IPEC-J2
OT  - Mitochondria
OT  - Oxidative phosphorylation
EDAT- 2021/03/17 06:00
MHDA- 2021/09/02 06:00
CRDT- 2021/03/16 17:34
PHST- 2021/03/03 00:00 [accepted]
PHST- 2021/03/17 06:00 [pubmed]
PHST- 2021/09/02 06:00 [medline]
PHST- 2021/03/16 17:34 [entrez]
AID - 10.1007/s00418-021-01981-2 [pii]
AID - 10.1007/s00418-021-01981-2 [doi]
PST - ppublish
SO  - Histochem Cell Biol. 2021 Jul;156(1):59-67. doi: 10.1007/s00418-021-01981-2. Epub 
      2021 Mar 16.