PMID- 33726673 OWN - NLM STAT- MEDLINE DCOM- 20210512 LR - 20210512 IS - 1471-2229 (Electronic) IS - 1471-2229 (Linking) VI - 21 IP - 1 DP - 2021 Mar 16 TI - Chromosome behavior during meiosis in pollen mother cells from Saccharum officinarum x Erianthus arundinaceus F(1) hybrids. PG - 139 LID - 10.1186/s12870-021-02911-z [doi] LID - 139 AB - BACKGROUND: In recent years, sugarcane has attracted increasing attention as an energy crop. Wild resources are widely used to improve the narrow genetic base of sugarcane. However, the infertility of F(1) hybrids between Saccharum officinarum (S. officinarum) and Erianthus arundinaceus (E. arundinaceus) has hindered sugarcane breeding efforts. To discover the cause of this infertility, we studied the hybridization process from a cytological perspective. RESULTS: We examined the meiotic process of pollen mother cells (PMCs) in three F(1) hybrids between S. officinarum and E. arundinaceus. Cytological analysis showed that the male parents, Hainan 92-77 and Hainan 92-105, had normal meiosis. However, the meiosis process in F(1) hybrids showed various abnormal phenomena, including lagging chromosomes, micronuclei, uneven segregation, chromosome bridges, and inability to form cell plates. Genomic in situ hybridization (GISH) showed unequal chromatin distribution during cell division. Interestingly, 96.70% of lagging chromosomes were from E. arundinaceus. Furthermore, fluorescence in situ hybridization (FISH) was performed using 45S rDNA and 5S rDNA as probes. Either 45S rDNA or 5S rDNA sites were lost during abnormal meiosis, and results of unequal chromosomal separation were also clearly observed in tetrads. CONCLUSIONS: Using cytogenetic analysis, a large number of meiotic abnormalities were observed in F(1). GISH further confirmed that 96.70% of the lagging chromosomes were from E. arundinaceus. Chromosome loss was found by further investigation of repeat sequences. Our findings provide insight into sugarcane chromosome inheritance to aid innovation and utilization in sugarcane germplasm resources. FAU - Li, Xueting AU - Li X AD - National Engineering Research Center for Sugarcane, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China. FAU - Huang, Fei AU - Huang F AD - National Engineering Research Center for Sugarcane, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China. FAU - Chai, Jin AU - Chai J AD - National Engineering Research Center for Sugarcane, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China. FAU - Wang, Qiusong AU - Wang Q AD - National Engineering Research Center for Sugarcane, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China. FAU - Yu, Fan AU - Yu F AD - National Engineering Research Center for Sugarcane, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China. FAU - Huang, Yongji AU - Huang Y AD - National Engineering Research Center for Sugarcane, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China. FAU - Wu, Jiayun AU - Wu J AD - Guangdong Key Laboratory of Sugarcane Improvement and Biorefinery, Guangdong Provincial Bioengineering Institute, Guangzhou, China. FAU - Wang, Qinnan AU - Wang Q AD - Key Lab of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China. FAU - Xu, Liangnian AU - Xu L AD - National Engineering Research Center for Sugarcane, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China. AD - Key Lab of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China. FAU - Zhang, Muqing AU - Zhang M AD - State Key Laboratory for Protection and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, 530004, China. FAU - Deng, Zuhu AU - Deng Z AUID- ORCID: 0000-0001-8609-7461 AD - National Engineering Research Center for Sugarcane, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China. dengzuhu@163.com. AD - Key Lab of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China. dengzuhu@163.com. AD - State Key Laboratory for Protection and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, 530004, China. dengzuhu@163.com. LA - eng GR - 31771863/National Natural Science Foundation of China/ GR - CARS-170106/Modern Agriculture Technology of China/ GR - SKLCUSA-a201912/State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources/ GR - SKLCUSA-b201806/State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources/ GR - KFA17168A/Science and Technology Innovation of Fujian Agriculture and Forestry University/ GR - KFA17525A/Science and Technology Innovation of Fujian Agriculture and Forestry University/ GR - KFA17169A/Science and Technology Innovation of Fujian Agriculture and Forestry University/ GR - 2018N1002/Science and technology program of Fujian Province/ PT - Journal Article DEP - 20210316 PL - England TA - BMC Plant Biol JT - BMC plant biology JID - 100967807 SB - IM MH - Chimera MH - China MH - Crops, Agricultural/genetics/growth & development MH - Genes, Plant MH - Genetic Variation MH - Genotype MH - Hybridization, Genetic MH - In Situ Hybridization, Fluorescence MH - Meiosis/*genetics/*physiology MH - Meristem/*genetics/growth & development MH - Poaceae/*genetics/*growth & development MH - Pollen/*genetics/growth & development MH - Saccharum/*genetics/*growth & development PMC - PMC7968283 OTO - NOTNLM OT - Cell genetics OT - Chromosome OT - FISH OT - Meiosis OT - Pollen COIS- The authors declare that they have no competing interests. EDAT- 2021/03/18 06:00 MHDA- 2021/05/13 06:00 PMCR- 2021/03/16 CRDT- 2021/03/17 05:40 PHST- 2019/12/24 00:00 [received] PHST- 2021/03/03 00:00 [accepted] PHST- 2021/03/17 05:40 [entrez] PHST- 2021/03/18 06:00 [pubmed] PHST- 2021/05/13 06:00 [medline] PHST- 2021/03/16 00:00 [pmc-release] AID - 10.1186/s12870-021-02911-z [pii] AID - 2911 [pii] AID - 10.1186/s12870-021-02911-z [doi] PST - epublish SO - BMC Plant Biol. 2021 Mar 16;21(1):139. doi: 10.1186/s12870-021-02911-z.