PMID- 33727863
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220421
IS  - 1179-1357 (Print)
IS  - 1179-1357 (Electronic)
IS  - 1179-1357 (Linking)
VI  - 13
DP  - 2021
TI  - Calcium Hydroxide Increases Human Umbilical Cord Mesenchymal Stem Cells 
      Expressions of Apoptotic Protease-Activating Factor-1, Caspase-3 and Caspase-9.
PG  - 59-65
LID - 10.2147/CCIDE.S284240 [doi]
AB  - PURPOSE: Calcium hydroxide is a gold standard dental material generally used for 
      pulpal and periapical therapy including regenerative endodontic procedures 
      because of its positive properties. However, evaluation about this material on 
      stem cells is limited. Human umbilical cord mesenchymal stem cells (HUCMSCs) are 
      potential to be used in regenerative therapy. Regenerative therapy needs a 
      sustainable cell supply to maintain its regenerative capacity. The aim of this 
      study was to ascertain the apoptosis result of calcium hydroxide on HUCMSCs 
      through the expression of apoptotic protease-activating factor-1 (APAF-1), 
      caspase-3, and caspase-9. MATERIALS AND METHODS: This study used a thawed frozen 
      stock of passage 5 HUCMSCs, grown in minimum essential medium (MEM) alpha 
      containing calcium hydroxide at concentration of 0.1 microgram/mL for 1, 3 and 7 
      days. Polyclonal antibody with fluorescence isothiocyanate (FITC) label was used 
      to evaluate the expressions. APAF-1, caspase-3, and caspase-9 expressions were 
      recorded and compared on every observation day using fluorescence microscope. 
      Analysis of variance was performed to analyze the significance among the results 
      of treatment groups. The results were concluded significant if p<0.05. RESULTS: 
      The addition of calcium hydroxide in MEM alpha medium increases HUCMSCs 
      expression of APAF-1, caspase-3 and caspase-9 significantly, compared to the 
      control group without calcium hydroxide (p<0.05) in all the times. Day 1 showed 
      the lowest increase followed by higher expressions on day 3 and day 7. 
      CONCLUSION: HUCMSCs express increased APAF-1, caspase-3 and caspase-9 after 
      in-vitro calcium hydroxide exposure. This should be considered when using calcium 
      hydroxide on HUCMSCs for regenerative procedures with regard to other positive 
      properties.
CI  - (c) 2021 Prasetyo et al.
FAU - Prasetyo, Eric Priyo
AU  - Prasetyo EP
AD  - Doctoral Program, Faculty of Medicine, Universitas Airlangga, Surabaya, 
      Indonesia.
AD  - Department of Conservative Dentistry, Faculty of Dental Medicine, Universitas 
      Airlangga, Surabaya, Indonesia.
FAU - Kuntjoro, Mefina
AU  - Kuntjoro M
AD  - Doctoral Program, Faculty of Medicine, Universitas Airlangga, Surabaya, 
      Indonesia.
AD  - Department of Prosthodontics, Faculty of Dental Medicine, Universitas Airlangga, 
      Surabaya, Indonesia.
FAU - Goenharto, Setyabudi
AU  - Goenharto S
AD  - Department of Conservative Dentistry, Faculty of Dental Medicine, Universitas 
      Airlangga, Surabaya, Indonesia.
FAU - Juniarti, Devi Eka
AU  - Juniarti DE
AD  - Department of Conservative Dentistry, Faculty of Dental Medicine, Universitas 
      Airlangga, Surabaya, Indonesia.
FAU - Cahyani, Febriastuti
AU  - Cahyani F
AD  - Department of Conservative Dentistry, Faculty of Dental Medicine, Universitas 
      Airlangga, Surabaya, Indonesia.
FAU - Hendrijantini, Nike
AU  - Hendrijantini N
AD  - Department of Prosthodontics, Faculty of Dental Medicine, Universitas Airlangga, 
      Surabaya, Indonesia.
FAU - Nugraha, Alexander Patera
AU  - Nugraha AP
AD  - Department of Orthodontics, Faculty of Dental Medicine, Universitas Airlangga, 
      Surabaya, Indonesia.
FAU - Hariyani, Ninuk
AU  - Hariyani N
AD  - Department of Dental Public Health, Faculty of Dental Medicine, Universitas 
      Airlangga, Surabaya, Indonesia.
FAU - Rantam, Fedik Abdul
AU  - Rantam FA
AUID- ORCID: 0000-0001-8182-1465
AD  - Stem Cell Research and Development Center, Universitas Airlangga, Surabaya, 
      Indonesia.
AD  - Laboratory of Virology, Department of Microbiology and Immunology, Faculty of 
      Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.
LA  - eng
PT  - Journal Article
DEP - 20210308
PL  - New Zealand
TA  - Clin Cosmet Investig Dent
JT  - Clinical, cosmetic and investigational dentistry
JID - 101579785
PMC - PMC7954029
OTO - NOTNLM
OT  - apoptosis
OT  - calcium hydroxide
OT  - caspases
OT  - mesenchymal stem cells
OT  - umbilical cord
COIS- The authors report no conflicts of interest in this work.
EDAT- 2021/03/18 06:00
MHDA- 2021/03/18 06:01
PMCR- 2021/03/08
CRDT- 2021/03/17 06:45
PHST- 2020/09/27 00:00 [received]
PHST- 2020/12/10 00:00 [accepted]
PHST- 2021/03/17 06:45 [entrez]
PHST- 2021/03/18 06:00 [pubmed]
PHST- 2021/03/18 06:01 [medline]
PHST- 2021/03/08 00:00 [pmc-release]
AID - 284240 [pii]
AID - 10.2147/CCIDE.S284240 [doi]
PST - epublish
SO  - Clin Cosmet Investig Dent. 2021 Mar 8;13:59-65. doi: 10.2147/CCIDE.S284240. 
      eCollection 2021.