PMID- 33727932
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210318
IS  - 1687-966X (Print)
IS  - 1687-9678 (Electronic)
VI  - 2021
DP  - 2021
TI  - Semaphorin3B Promotes Proliferation and Osteogenic Differentiation of Bone Marrow 
      Mesenchymal Stem Cells in a High-Glucose Microenvironment.
PG  - 6637176
LID - 10.1155/2021/6637176 [doi]
LID - 6637176
AB  - Bone marrow mesenchymal stem cells (BMSCs) play an essential role in osteogenesis 
      and bone metabolism and have already been recognized as one of the most popular 
      seed cells for bone tissue engineering for bone diseases. However, high-glucose 
      (HG) conditions in type 2 diabetes mellitus (T2DM) exert deleterious effects on 
      BMSC proliferation and osteogenic differentiation. Semaphorin 3B (Sema3B) 
      increases osteoblast differentiation in bone metabolism. Here, we determined the 
      role of Sema3B in the proliferation and osteogenic differentiation of BMSCs in 
      the HG microenvironment. The HG microenvironment decreased Sema3B expression in 
      BMSCs. Moreover, HG inhibited BMSC proliferation. Furthermore, HG inhibited 
      osteogenic differentiation in BMSCs by decreasing the expression of bone 
      formation markers, alkaline phosphatase (ALP) activity, and mineralization. 
      However, the administration of recombinant Sema3B reversed all of these effects. 
      Moreover, our study found that Sema3B could activate the Akt pathway in BMSCs. 
      Sema3B rescues defects in BMSC proliferation and osteogenic differentiation in 
      the HG microenvironment by activating the Akt pathway. These effects were 
      significantly reduced by treatment with an Akt inhibitor. Together, these 
      findings demonstrate that Sema3B promotes the proliferation and osteogenic 
      differentiation of BMSCs via the Akt pathway under HG conditions. Our study 
      provides new insights into the potential ability of Sema3B to ameliorate BMSC 
      proliferation and osteogenic differentiation in an HG microenvironment.
CI  - Copyright (c) 2021 Quan Xing et al.
FAU - Xing, Quan
AU  - Xing Q
AUID- ORCID: 0000-0001-6367-5863
AD  - Department of Zhujiang New Town Clinic, Guanghua School and Hospital of 
      Stomatology, Sun Yat-sen University, Guangzhou 510055, China.
AD  - Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, 
      Guangzhou, China.
FAU - Feng, Jingyi
AU  - Feng J
AUID- ORCID: 0000-0001-5064-7484
AD  - Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, 
      Guangzhou, China.
AD  - Department of Operative Dentistry and Endodontics, Guanghua School and Hospital 
      of Stomatology, Sun Yat-sen University, Guangzhou 510055, China.
FAU - Zhang, Xiaolei
AU  - Zhang X
AUID- ORCID: 0000-0003-2410-179X
AD  - Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, 
      Guangzhou, China.
AD  - Department of Operative Dentistry and Endodontics, Guanghua School and Hospital 
      of Stomatology, Sun Yat-sen University, Guangzhou 510055, China.
LA  - eng
PT  - Journal Article
DEP - 20210226
PL  - United States
TA  - Stem Cells Int
JT  - Stem cells international
JID - 101535822
PMC - PMC7935575
COIS- No conflicts of interest exist regarding the submission of this manuscript, and 
      all authors approved the manuscript for publication.
EDAT- 2021/03/18 06:00
MHDA- 2021/03/18 06:01
PMCR- 2021/02/26
CRDT- 2021/03/17 06:47
PHST- 2020/12/02 00:00 [received]
PHST- 2021/01/22 00:00 [revised]
PHST- 2021/02/08 00:00 [accepted]
PHST- 2021/03/17 06:47 [entrez]
PHST- 2021/03/18 06:00 [pubmed]
PHST- 2021/03/18 06:01 [medline]
PHST- 2021/02/26 00:00 [pmc-release]
AID - 10.1155/2021/6637176 [doi]
PST - epublish
SO  - Stem Cells Int. 2021 Feb 26;2021:6637176. doi: 10.1155/2021/6637176. eCollection 
      2021.