PMID- 33728093
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210318
IS  - 2090-8865 (Print)
IS  - 2090-8873 (Electronic)
IS  - 2090-8873 (Linking)
VI  - 2021
DP  - 2021
TI  - A Fast and Validated HPLC Method for Simultaneous Determination of Dopamine, 
      Dobutamine, Phentolamine, Furosemide, and Aminophylline in Infusion Samples and 
      Injection Formulations.
PG  - 8821126
LID - 10.1155/2021/8821126 [doi]
LID - 8821126
AB  - A simple, fast, and validated HPLC method was developed for the simultaneous 
      quantization of five cardiovascular agents: dopamine (DPM), dobutamine (DBM), 
      phentolamine (PTM), furosemide (FSM), and aminophylline (APL) either in infusion 
      samples or in an injection dosage form. The proposed method was achieved with a 
      150 mm x 4.6 mm, 5.0 mum C(18) column, by using a simple linear gradient. Mobile 
      phase A was buffer (50 mM KH(2)PO(4)) and mobile Phase B was acetonitrile at a 
      flow rate of 1.0 mL/min. The column temperature was kept at 30 degrees C, and the 
      injection volume was 20 muL. All analytes were separated simultaneously at a 
      retention time (tr) of 3.93, 5.84, 7.06, 8.76, and 9.67 min for DPM, DBM, PTM, 
      FSM, and APL, respectively, with a total run time of less than 15.0 min. The 
      proposed method was validated according to ICH guidelines with respect to 
      accuracy, precision, linearity, limit of detection, limit of quantitation, and 
      robustness. Linearity was obtained over a concentration range of 12.0-240.0, 
      12.0-240.0, 20.0-200.0, 6.0-240.0, and 10.0-200.0 mug/mL DPM, DBM, PTM, FSM, and 
      APL, respectively. Interday and intraday accuracy and precision data were 
      recorded in the acceptable limits. The new method has successfully been applied 
      for quantification of all five drugs in their injection dosage form, infusion 
      samples, and for evaluation of the stability of investigated drugs in mixtures 
      for endovenous use. The results of the stability study showed that mixtures of 
      DPM, DBM, PTM, FSM, and APL in 5% glucose or 0.9% sodium chloride injection were 
      stable for 48 hours when stored in polypropylene syringes at 25 degrees C.
CI  - Copyright (c) 2021 Fuchao Chen et al.
FAU - Chen, Fuchao
AU  - Chen F
AUID- ORCID: 0000-0001-9738-6744
AD  - School of Pharmacy, Xi'an Jiaotong University, Xi'an 710061, Shanxi, China.
AD  - Sinopharm Dongfeng General Hospital, Hubei University of Medicine, Shiyan, Hubei 
      442008, China.
FAU - Fang, Baoxia
AU  - Fang B
AUID- ORCID: 0000-0002-9299-8271
AD  - Sinopharm Dongfeng General Hospital, Hubei University of Medicine, Shiyan, Hubei 
      442008, China.
FAU - Wang, Sicen
AU  - Wang S
AUID- ORCID: 0000-0002-2757-3277
AD  - School of Pharmacy, Xi'an Jiaotong University, Xi'an 710061, Shanxi, China.
AD  - Sinopharm Dongfeng General Hospital, Hubei University of Medicine, Shiyan, Hubei 
      442008, China.
LA  - eng
PT  - Journal Article
DEP - 20210227
PL  - Egypt
TA  - J Anal Methods Chem
JT  - Journal of analytical methods in chemistry
JID - 101575377
PMC - PMC7936887
COIS- The authors declare no conflicts of interest.
EDAT- 2021/03/18 06:00
MHDA- 2021/03/18 06:01
PMCR- 2021/02/27
CRDT- 2021/03/17 06:50
PHST- 2020/07/31 00:00 [received]
PHST- 2020/11/30 00:00 [revised]
PHST- 2021/02/11 00:00 [accepted]
PHST- 2021/03/17 06:50 [entrez]
PHST- 2021/03/18 06:00 [pubmed]
PHST- 2021/03/18 06:01 [medline]
PHST- 2021/02/27 00:00 [pmc-release]
AID - 10.1155/2021/8821126 [doi]
PST - epublish
SO  - J Anal Methods Chem. 2021 Feb 27;2021:8821126. doi: 10.1155/2021/8821126. 
      eCollection 2021.