PMID- 33729516
OWN - NLM
STAT- MEDLINE
DCOM- 20220228
LR  - 20220318
IS  - 1532-2548 (Electronic)
IS  - 0032-0889 (Print)
IS  - 0032-0889 (Linking)
VI  - 186
IP  - 2
DP  - 2021 Jun 11
TI  - GTP binding by Arabidopsis extra-large G protein 2 is not essential for its 
      functions.
PG  - 1240-1253
LID - 10.1093/plphys/kiab119 [doi]
AB  - The extra-large guanosine-5'-triphosphate (GTP)-binding protein 2, XLG2, is an 
      unconventional Galpha subunit of the Arabidopsis (Arabidopsis thaliana) 
      heterotrimeric GTP-binding protein complex with a major role in plant defense. In 
      vitro biochemical analyses and molecular dynamic simulations show that affinity 
      of XLG2 for GTP is two orders of magnitude lower than that of the conventional 
      Galpha, AtGPA1. Here we tested the physiological relevance of GTP binding by XLG2. We 
      generated an XLG2(T476N) variant with abolished GTP binding, as confirmed by in 
      vitro GTPgammaS binding assay. Yeast three-hybrid, bimolecular fluorescence 
      complementation, and split firefly-luciferase complementation assays revealed 
      that the nucleotide-depleted XLG2(T476N) retained wild-type XLG2-like 
      interactions with the Gbetagamma dimer and defense-related receptor-like kinases. Both 
      wild-type and nucleotide-depleted XLG2(T476N) restored the defense responses 
      against Fusarium oxysporum and Pseudomonas syringae compromised in the xlg2 xlg3 
      double mutant. Additionally, XLG2(T476N) was fully functional restoring stomatal 
      density, root growth, and sensitivity to NaCl, but failed to complement impaired 
      germination and vernalization-induced flowering. We conclude that XLG2 is able to 
      function in a GTP-independent manner and discuss its possible mechanisms of 
      action.
CI  - (c) American Society of Plant Biologists 2021. All rights reserved. For 
      permissions, please email: journals.permissions@oup.com.
FAU - Maruta, Natsumi
AU  - Maruta N
AUID- ORCID: 0000-0002-6246-6346
AD  - Plant Genetic Engineering Laboratory, School of Agriculture and Food Sciences, 
      University of Queensland, Brisbane, QLD, 4072, Australia.
AD  - School of Chemistry and Molecular Biosciences, Institute for Molecular Bioscience 
      and Australian Infectious Diseases Research Centre, University of Queensland, 
      Brisbane, QLD 4072, Australia.
FAU - Trusov, Yuri
AU  - Trusov Y
AUID- ORCID: 0000-0001-8215-5492
AD  - Plant Genetic Engineering Laboratory, School of Agriculture and Food Sciences, 
      University of Queensland, Brisbane, QLD, 4072, Australia.
FAU - Urano, Daisuke
AU  - Urano D
AUID- ORCID: 0000-0003-4541-1076
AD  - Temasek Life Sciences Laboratory, 1 Research Link, National University of 
      Singapore, Singapore 117604, Singapore.
FAU - Chakravorty, David
AU  - Chakravorty D
AUID- ORCID: 0000-0002-6591-4853
AD  - Department of Biology, Pennsylvania State University, University Park, 
      Pennsylvania 16802, USA.
FAU - Assmann, Sarah M
AU  - Assmann SM
AUID- ORCID: 0000-0003-4541-1594
AD  - Department of Biology, Pennsylvania State University, University Park, 
      Pennsylvania 16802, USA.
FAU - Jones, Alan M
AU  - Jones AM
AUID- ORCID: 0000-0002-2365-6462
AD  - Department of Biology, University of North Carolina, Chapel Hill, North Carolina 
      27599, USA.
AD  - Department of Pharmacology, University of North Carolina, Chapel Hill, North 
      Carolina 27599, USA.
FAU - Botella, Jose R
AU  - Botella JR
AUID- ORCID: 0000-0002-4446-3432
AD  - Plant Genetic Engineering Laboratory, School of Agriculture and Food Sciences, 
      University of Queensland, Brisbane, QLD, 4072, Australia.
LA  - eng
GR  - R01 GM065989/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Plant Physiol
JT  - Plant physiology
JID - 0401224
RN  - 0 (Arabidopsis Proteins)
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - EC 3.6.5.1 (Heterotrimeric GTP-Binding Proteins)
RN  - EC 3.6.5.1 (XLG2 protein, Arabidopsis)
RN  - Fusarium oxysporum
SB  - IM
MH  - Arabidopsis/enzymology/*genetics/immunology/microbiology
MH  - Arabidopsis Proteins/genetics/*metabolism
MH  - Fusarium/*physiology
MH  - Guanosine Triphosphate/metabolism
MH  - Heterotrimeric GTP-Binding Proteins/genetics/*metabolism
MH  - Plant Diseases/*immunology/microbiology
MH  - Pseudomonas syringae/*physiology
PMC - PMC8195506
EDAT- 2021/03/18 06:00
MHDA- 2022/03/01 06:00
PMCR- 2022/03/17
CRDT- 2021/03/17 12:36
PHST- 2021/02/19 00:00 [accepted]
PHST- 2021/02/10 00:00 [received]
PHST- 2021/03/18 06:00 [pubmed]
PHST- 2022/03/01 06:00 [medline]
PHST- 2021/03/17 12:36 [entrez]
PHST- 2022/03/17 00:00 [pmc-release]
AID - 6174699 [pii]
AID - kiab119 [pii]
AID - 10.1093/plphys/kiab119 [doi]
PST - ppublish
SO  - Plant Physiol. 2021 Jun 11;186(2):1240-1253. doi: 10.1093/plphys/kiab119.