PMID- 33732319
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210320
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 21
IP  - 4
DP  - 2021 Apr
TI  - Upregulated expression of leukocyte immunoglobulin-like receptor A3 in patients 
      with severe aplastic anemia.
PG  - 346
LID - 10.3892/etm.2021.9777 [doi]
LID - 346
AB  - Severe aplastic anemia (SAA) is a rare and potentially life-threatening disease 
      characterized by pancytopenia and bone marrow (BM) hypoplasia. In a previous 
      study by our group, increased expression of leukocyte immunoglobulin-like 
      receptors A (LILRA), LILRA3 in myeloid dendritic cells (mDCs) and LILRA5 in 
      CD34(+) cells in SAA was detected using proteomics techniques, highlighting their 
      potential role in disease pathogenesis. In the present study, the expression of 
      LILRA1-6 mRNA was assessed in the BM mononuclear cells of patients with SAA using 
      reverse transcription-quantitative (RT-q)PCR. The expression of homogenic LILRA3 
      and LILRA5 isoform on mDCs, as well as CD34(+), CD3(+)CD8(+), CD19(+) and CD14(+) 
      cells, was detected using flow cytometry. mDCs were then induced, cultured and 
      sorted. The expression of LILRA3 was confirmed using RT-qPCR and western blot 
      analyses. The serum levels of soluble LILRA3 were measured using ELISA. 
      Furthermore, the relationship between LILRA3 expression and disease severity was 
      assessed. The results indicated increased LILRA3 mRNA expression in patients with 
      SAA. The percentage of LILRA3(+) in BM mDCs and CD34(+) cells was increased. 
      Compared with controls, the relative LILRA3 mRNA expression and the relative 
      protein intensity were highly increased in SAA mDCs. The serum LILRA3 levels in 
      patients with SAA were also increased. The proportion of LILRA3(+)CD11C(+) human 
      leukocyte antigen (HLA)-DR(+)/CD11C(+)HLA-DR(+) cells was positively correlated 
      with the ratio of LILRA3(+)CD34(+)/CD34(+) cells and the expression of LILRA3 
      mRNA. Taken together, the expression of LILRA3 on mDCs of patients with SAA was 
      increased, which may affect the function of mDCs. LILRA3 may have a significant 
      role in the immune pathogenesis of SAA.
CI  - Copyright: (c) Yu et al.
FAU - Yu, Hong
AU  - Yu H
AD  - Department of Hematology, Tianjin Medical University General Hospital, Tianjin 
      300052, P.R. China.
FAU - Liu, Hui
AU  - Liu H
AD  - Department of Hematology, Tianjin Medical University General Hospital, Tianjin 
      300052, P.R. China.
FAU - Zhao, Yang
AU  - Zhao Y
AD  - Department of Hematology, Tianjin Medical University General Hospital, Tianjin 
      300052, P.R. China.
FAU - Wang, Huaquan
AU  - Wang H
AD  - Department of Hematology, Tianjin Medical University General Hospital, Tianjin 
      300052, P.R. China.
FAU - Liu, Chunyan
AU  - Liu C
AD  - Department of Hematology, Tianjin Medical University General Hospital, Tianjin 
      300052, P.R. China.
FAU - Qi, Weiwei
AU  - Qi W
AD  - Department of Hematology, Tianjin Medical University General Hospital, Tianjin 
      300052, P.R. China.
FAU - Liu, Zhaoyun
AU  - Liu Z
AD  - Department of Hematology, Tianjin Medical University General Hospital, Tianjin 
      300052, P.R. China.
FAU - Sun, Yingying
AU  - Sun Y
AD  - Department of Hematology, Tianjin Medical University General Hospital, Tianjin 
      300052, P.R. China.
FAU - Gao, Shan
AU  - Gao S
AD  - Department of Hematology, Tianjin Medical University General Hospital, Tianjin 
      300052, P.R. China.
FAU - Tao, Jinglian
AU  - Tao J
AD  - Department of Hematology, Tianjin Medical University General Hospital, Tianjin 
      300052, P.R. China.
FAU - Fu, Rong
AU  - Fu R
AD  - Department of Hematology, Tianjin Medical University General Hospital, Tianjin 
      300052, P.R. China.
FAU - Shao, Zonghong
AU  - Shao Z
AD  - Department of Hematology, Tianjin Medical University General Hospital, Tianjin 
      300052, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20210211
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC7903422
OTO - NOTNLM
OT  - leukocyte immunoglobulin-like receptor A3
OT  - myeloid dendritic cells
OT  - proteomics
OT  - severe aplastic anemia
COIS- The authors declare that they have no competing interests.
EDAT- 2021/03/19 06:00
MHDA- 2021/03/19 06:01
PMCR- 2021/02/11
CRDT- 2021/03/18 06:48
PHST- 2020/06/24 00:00 [received]
PHST- 2020/11/03 00:00 [accepted]
PHST- 2021/03/18 06:48 [entrez]
PHST- 2021/03/19 06:00 [pubmed]
PHST- 2021/03/19 06:01 [medline]
PHST- 2021/02/11 00:00 [pmc-release]
AID - ETM-0-0-09777 [pii]
AID - 10.3892/etm.2021.9777 [doi]
PST - ppublish
SO  - Exp Ther Med. 2021 Apr;21(4):346. doi: 10.3892/etm.2021.9777. Epub 2021 Feb 11.