PMID- 33732340
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210320
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 21
IP  - 4
DP  - 2021 Apr
TI  - Knockdown of lncRNA NEAT1 expression inhibits cell migration, invasion and EMT by 
      regulating the miR-24-3p/LRG1 axis in retinoblastoma cells.
PG  - 367
LID - 10.3892/etm.2021.9798 [doi]
LID - 367
AB  - Retinoblastoma (RB) is the most common primary intraocular cancer type that 
      occurs during retinal development in childhood. Previous studies have reported 
      that long non-coding RNAs (lncRNAs) are involved in the development of RB. 
      Therefore, the aim of the present study was to investigate the effects and 
      underlying regulatory mechanisms of nuclear paraspeckle assembly transcript 1 
      (NEAT1) in RB. The expression levels of NEAT1, microRNA (miR)-24-3p and 
      leucine-rich-alpha-2-glycoprotein (LRG1) were detected using reverse 
      transcription-quantitative PCR (RT-qPCR). Moreover, the protein expression levels 
      of LRG1, matrix metalloproteinase 9, N-cadherin and E-cadherin were detected via 
      western blotting. Furthermore, cell migration and invasion abilities were 
      evaluated via Transwell assays. The targeting relationships between miR-24-3p and 
      NEAT1 or LRG1 were predicted using online software and confirmed via 
      dual-luciferase reporter assay. In the present study, NEAT1 and LRG1 were 
      upregulated, and miR-24-3p was downregulated in RB tissues and cells compared 
      with the corresponding healthy tissues and cells. Moreover, miR-24-3p was 
      identified as a target of NEAT and LRG1 was demonstrated to be a direct target 
      gene of miR-24-3p. Knockdown of NEAT1 or LRG1 significantly suppressed RB cell 
      migration and invasion ability, while the effects were reversed by an miR-24-3p 
      inhibitor. In addition, the downregulation of LRG1 caused by miR-24-3p was 
      restored following the overexpression of NEAT1 in RB cells. It was also 
      demonstrated that NEAT1 knockdown inhibited the epithelial-to-mesenchymal 
      transition (EMT) pathway by inhibiting the expression of LRG via targeting 
      miR-24-3p. In conclusion, the present results suggest that silencing of NEAT1 
      suppresses cell migration, invasion and the EMT process by downregulating LRG1 
      expression via sponging miR-24-3p in RB, thus indicating that NEAT1 may be a 
      potential candidate for RB treatment.
CI  - Copyright: (c) Luan et al.
FAU - Luan, Lan
AU  - Luan L
AD  - Department of Ophthalmology, Renmin Hospital, Hubei University of Medicine, 
      Shiyan, Hubei 442000, P.R. China.
FAU - Hu, Qiang
AU  - Hu Q
AD  - Department of Ophthalmology, Renmin Hospital, Hubei University of Medicine, 
      Shiyan, Hubei 442000, P.R. China.
FAU - Wang, Yan
AU  - Wang Y
AD  - Department of Ophthalmology, Renmin Hospital, Hubei University of Medicine, 
      Shiyan, Hubei 442000, P.R. China.
FAU - Lu, Lu
AU  - Lu L
AD  - Department of Ophthalmology, Renmin Hospital, Hubei University of Medicine, 
      Shiyan, Hubei 442000, P.R. China.
FAU - Ling, Jiaojiao
AU  - Ling J
AD  - Department of Ophthalmology, Renmin Hospital, Hubei University of Medicine, 
      Shiyan, Hubei 442000, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20210218
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC7903428
OTO - NOTNLM
OT  - epithelial-to-mesenchymal transition
OT  - leucine-rich-alpha-2-glycoprotein
OT  - long non-coding RNA nuclear paraspeckle assembly transcript 1
OT  - microRNA-24-3p
OT  - retinoblastoma
COIS- The authors declare that they have no competing interests.
EDAT- 2021/03/19 06:00
MHDA- 2021/03/19 06:01
PMCR- 2021/02/18
CRDT- 2021/03/18 06:48
PHST- 2019/09/07 00:00 [received]
PHST- 2020/04/17 00:00 [accepted]
PHST- 2021/03/18 06:48 [entrez]
PHST- 2021/03/19 06:00 [pubmed]
PHST- 2021/03/19 06:01 [medline]
PHST- 2021/02/18 00:00 [pmc-release]
AID - ETM-0-0-09798 [pii]
AID - 10.3892/etm.2021.9798 [doi]
PST - ppublish
SO  - Exp Ther Med. 2021 Apr;21(4):367. doi: 10.3892/etm.2021.9798. Epub 2021 Feb 18.