PMID- 33734700
OWN - NLM
STAT- MEDLINE
DCOM- 20210514
LR  - 20210514
IS  - 1520-5118 (Electronic)
IS  - 0021-8561 (Linking)
VI  - 69
IP  - 12
DP  - 2021 Mar 31
TI  - l-Arabinose Attenuates Gliadin-Induced Food Allergy via Regulation of Th1/Th2 
      Balance and Upregulation of Regulatory T Cells in Mice.
PG  - 3638-3646
LID - 10.1021/acs.jafc.0c07167 [doi]
AB  - Gliadins are the main cause of wheat allergies, and the prevalence of gliadin 
      allergy has increased in many countries. l-Arabinose, a kind of plant-specific 
      five-carbon aldose, possesses beneficial effects on food allergy to gliadins. 
      This study investigated the antiallergic activities and underlying mechanisms of 
      l-arabinose in a wheat gliadin-sensitized mouse model. BALB/c mice were 
      sensitized to gliadin by intraperitoneal injections with gliadin followed by 
      being given a gliadin challenge. l-arabinose-treated mice exhibited a marked 
      reduction in the productions of total immunoglobulin E (IgE), gliadin-specific 
      IgE, gliadin-specific IgG1, and histamine, with an increase in IgG2a level as 
      compared with gliadin-sensitized mice. Beside that, a significant decrease in 
      Th2-related cytokine level, IL-4, and an increase in Th1-related cytokine level, 
      IFN-gamma, in the serum and splenocytes were observed after treatment with 
      l-arabinose. l-Arabinose treatment also improved the imbalance of Th1/Th2 immune 
      response on the basis of the expression levels of related cytokines and key 
      transcription factors in the small intestine and spleen of sensitized mice. In 
      addition, gliadin-induced intestinal barrier impairment was blocked by 
      l-arabinose treatment via regulation of TJ proteins and suppression of p38 MAPK 
      and p65 NF-kappaB inflammation signaling pathways. Notably, the results confirmed 
      that l-arabinose treatment increased CD4(+) Foxp3(+) T cell populations and 
      Treg-related factors associated with increased expression of IL-2 and activation 
      of STAT5 in gliadin-sensitized mice. In conclusion, l-arabinose attenuated the 
      gliadin-induced allergic symptoms via maintenance of Th1/Th2 immune balance and 
      regulation of Treg cells in a gliadin-induced mouse model, suggesting l-arabinose 
      could be used as a promising agent to alleviate gliadin allergy.
FAU - Wang, Yu
AU  - Wang Y
AD  - State Key Laboratory of Food Science and Technology, School of Food Science and 
      Technology, Jiangnan University, Wuxi 214122, China.
FAU - Sun, Juan
AU  - Sun J
AD  - State Key Laboratory of Food Science and Technology, School of Food Science and 
      Technology, Jiangnan University, Wuxi 214122, China.
FAU - Xue, Lamei
AU  - Xue L
AD  - State Key Laboratory of Food Science and Technology, School of Food Science and 
      Technology, Jiangnan University, Wuxi 214122, China.
FAU - Liu, Jinxin
AU  - Liu J
AD  - State Key Laboratory of Food Science and Technology, School of Food Science and 
      Technology, Jiangnan University, Wuxi 214122, China.
FAU - Nie, Chenzhipeng
AU  - Nie C
AD  - State Key Laboratory of Food Science and Technology, School of Food Science and 
      Technology, Jiangnan University, Wuxi 214122, China.
FAU - Fan, Mingcong
AU  - Fan M
AD  - State Key Laboratory of Food Science and Technology, School of Food Science and 
      Technology, Jiangnan University, Wuxi 214122, China.
FAU - Qian, Haifeng
AU  - Qian H
AD  - State Key Laboratory of Food Science and Technology, School of Food Science and 
      Technology, Jiangnan University, Wuxi 214122, China.
FAU - Zhang, Duo
AU  - Zhang D
AD  - Clinical and Experimental Therapeutics, College of Pharmacy, University of 
      Georgia and Charlie Norwood VA Medical Center, Augusta, Georgia 30912, United 
      States.
FAU - Ying, Hao
AU  - Ying H
AD  - Chinese Academy of Sciences Key Laboratory of Nutrition, Metabolism and Food 
      Safety, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 
      Shanghai 200031, China.
FAU - Li, Yan
AU  - Li Y
AUID- ORCID: 0000-0002-9402-1093
AD  - State Key Laboratory of Food Science and Technology, School of Food Science and 
      Technology, Jiangnan University, Wuxi 214122, China.
FAU - Wang, Li
AU  - Wang L
AD  - State Key Laboratory of Food Science and Technology, School of Food Science and 
      Technology, Jiangnan University, Wuxi 214122, China.
LA  - eng
PT  - Journal Article
DEP - 20210318
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (Cytokines)
RN  - 9007-90-3 (Gliadin)
RN  - B40ROO395Z (Arabinose)
SB  - IM
MH  - Animals
MH  - Arabinose
MH  - Cytokines/genetics/metabolism
MH  - *Food Hypersensitivity
MH  - *Gliadin
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - T-Lymphocytes, Regulatory
MH  - Th1 Cells
MH  - Th2 Cells
MH  - Up-Regulation
OTO - NOTNLM
OT  - Th2
OT  - allergy
OT  - l-Arabinose
OT  - regulatory T cells
OT  - wheat gliadins
EDAT- 2021/03/19 06:00
MHDA- 2021/05/15 06:00
CRDT- 2021/03/18 17:18
PHST- 2021/03/19 06:00 [pubmed]
PHST- 2021/05/15 06:00 [medline]
PHST- 2021/03/18 17:18 [entrez]
AID - 10.1021/acs.jafc.0c07167 [doi]
PST - ppublish
SO  - J Agric Food Chem. 2021 Mar 31;69(12):3638-3646. doi: 10.1021/acs.jafc.0c07167. 
      Epub 2021 Mar 18.