PMID- 33737111
OWN - NLM
STAT- MEDLINE
DCOM- 20210914
LR  - 20210914
IS  - 1873-6351 (Electronic)
IS  - 0278-6915 (Linking)
VI  - 151
DP  - 2021 May
TI  - Effects of alcohol intake on cognitive function and beta-amyloid protein in APP/PS1 
      transgenic mice.
PG  - 112105
LID - S0278-6915(21)00138-1 [pii]
LID - 10.1016/j.fct.2021.112105 [doi]
AB  - To investigate the effects of alcohol intake on cognitive function and beta-amyloid 
      protein (Abeta) in APP/PS1 double-transgenic mice with Alzheimer's disease (AD). 
      Sixty APP/PS1 transgenic male mice were randomized into seven groups: control 
      group, 0.5% alcohol group, 1% alcohol group, 2% alcohol group, 3% alcohol group, 
      and 4% alcohol group, with 10 non-transgenic B6C3F1 mice of the same genetic 
      background as the negative control group. All mice were pair-fed a liquid diet 
      containing alcohol before assessment of learning and memory using the Y-maze 
      test, and of Abeta content and related enzyme activity on them. Immunohistochemistry 
      was used to detect the expression of Abeta(1-42), Abeta(1-40), and beta-amyloid precursor 
      protein (beta-APP) in the cerebral cortex. 3%, and 4% alcohol intake significantly 
      impaired the learning and memory abilities. 2%, 3%, and 4% alcohol groups 
      indicated a remarkable change in Abeta(1-42) content, alpha-secretase and gamma-secretase 
      activities in the hippocampus, and beta-APP in the cortex; 3% and 4% alcohol groups 
      showed a significant increase in Abeta(1-42) content, beta-site amyloid cleavage enzyme 
      1 (BACE1) activity, and a significant decrease in alpha-secretase activity in the 
      hippocampus or cortex; 2% and 3% alcohol groups showed a significant increase in 
      Abeta(1-40) content in the hippocampus or cortex; and 2%, 3%, and 4% alcohol groups 
      showed an increase in the expression of Abeta(1-42), Abeta(1-40,) and beta-APP in the 
      cortex; 1% alcohol groups showed a significant decline in the levels of Abeta(1-42), 
      Abeta(1-40), beta-APP, and BACE1 activity in the hippocampus, and gamma-secretase activity 
      in the hippocampus and cortex, and 1% alcohol group showed a significant increase 
      of alpha-secretase activity in the hippocampus. Besides, 0.5% alcohol showed 
      statistically significant effects on the reduction of BACE1 and gamma-secretase 
      activities in the hippocampus. Long-term intake of high-dose alcohol can induce 
      cognitive deficits and improve the activity of beta-APP decomposition-related 
      enzymes, increase Abeta content and deposition, and initiate AD progression, while 
      long-term intake of low-dose alcohol can antagonize excessive production of Abeta 
      and slow down AD progression.
CI  - Copyright (c) 2021 Elsevier Ltd. All rights reserved.
FAU - Gong, Yu-Shi
AU  - Gong YS
AD  - School of Food Science, Guangdong Pharmaceutical University, Zhongshan, 528458, 
      China. Electronic address: tortoise39@163.com.
FAU - Hou, Fang-Li
AU  - Hou FL
AD  - School of Food Science, Guangdong Pharmaceutical University, Zhongshan, 528458, 
      China.
FAU - Guo, Juan
AU  - Guo J
AD  - School of Food Science, Guangdong Pharmaceutical University, Zhongshan, 528458, 
      China.
FAU - Lin, Lin
AU  - Lin L
AD  - School of Food Science, Guangdong Pharmaceutical University, Zhongshan, 528458, 
      China.
FAU - Zhu, Fu-Yong
AU  - Zhu FY
AD  - School of Food Science, Guangdong Pharmaceutical University, Zhongshan, 528458, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20210316
PL  - England
TA  - Food Chem Toxicol
JT  - Food and chemical toxicology : an international journal published for the British 
      Industrial Biological Research Association
JID - 8207483
RN  - 0 (Amyloid beta-Peptides)
RN  - 0 (Amyloid beta-Protein Precursor)
RN  - 0 (Presenilin-1)
RN  - 0 (presenilin 1, mouse)
RN  - 3K9958V90M (Ethanol)
RN  - EC 3.4.- (Amyloid Precursor Protein Secretases)
RN  - EC 3.4.23.- (Aspartic Acid Endopeptidases)
RN  - EC 3.4.23.46 (Bace1 protein, mouse)
SB  - IM
MH  - *Alcohol Drinking
MH  - Amyloid Precursor Protein Secretases/metabolism
MH  - Amyloid beta-Peptides/*metabolism
MH  - Amyloid beta-Protein Precursor/*genetics
MH  - Animals
MH  - Aspartic Acid Endopeptidases/metabolism
MH  - Brain/enzymology/metabolism
MH  - *Cognition
MH  - Dose-Response Relationship, Drug
MH  - Ethanol/administration & dosage
MH  - Male
MH  - Mice
MH  - Mice, Transgenic
MH  - Presenilin-1/*genetics
OTO - NOTNLM
OT  - Alcohol
OT  - Alzheimer's disease
OT  - Brain
OT  - Learning and memory
OT  - beta-amyloid protein
EDAT- 2021/03/20 06:00
MHDA- 2021/09/15 06:00
CRDT- 2021/03/19 06:17
PHST- 2020/10/30 00:00 [received]
PHST- 2021/02/08 00:00 [revised]
PHST- 2021/03/05 00:00 [accepted]
PHST- 2021/03/20 06:00 [pubmed]
PHST- 2021/09/15 06:00 [medline]
PHST- 2021/03/19 06:17 [entrez]
AID - S0278-6915(21)00138-1 [pii]
AID - 10.1016/j.fct.2021.112105 [doi]
PST - ppublish
SO  - Food Chem Toxicol. 2021 May;151:112105. doi: 10.1016/j.fct.2021.112105. Epub 2021 
      Mar 16.