PMID- 33737587
OWN - NLM
STAT- MEDLINE
DCOM- 20211011
LR  - 20211011
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 11
IP  - 1
DP  - 2021 Mar 18
TI  - Evaluation of polyhexamethylene guanidine-induced lung injuries by chest CT, 
      pathologic examination, and RNA sequencing in a rat model.
PG  - 6318
LID - 10.1038/s41598-021-85662-z [doi]
LID - 6318
AB  - Our aim was to correlate chest CT and pathologic findings of polyhexamethylene 
      guanidine phosphate (PHMG)-induced lung injuries in a rat model, to determine 
      whether PHMG exposure causes lung tumors, and to explore genetic alterations 
      according to PHMG exposure under the guidance of CT. A PHMG solution was 
      intratracheally administrated to 40 male rats. Chest CT was carried out in all 
      rats and both lungs were collected for histopathologic evaluation. At 4- and 
      8-weeks post-instillation, one lobe of the right lung from 3 rats was subjected 
      to RNA sequencing. At least one abnormal CT finding was found in all rats at all 
      weeks. The major CT findings were inflammation, fibrosis, and tumors in the 
      pathologic analysis, where significant changes were observed over time. The lung 
      lesions remained persistent after 8 weeks of PHMG exposure. In the pathologic 
      analysis, the extent/severity of inflammation did not show statistically 
      significant changes over time, whereas the extent/severity of fibrosis increased 
      continuously up to 6 weeks after PHMG exposure and then decreased significantly 
      at 8 weeks. Bronchiolar-alveolar adenomas which have malignant potential were 
      found in 50% of rats at 6 and 8 weeks after PHMG exposure. Also, several genes 
      associated with lung cancer, acute lung injury, and pulmonary fibrosis were 
      detected. Our study revealed that PHMG-induced lung injury and its changes 
      according to the number of weeks after exposure were demonstrated using chest CT 
      and pathologic evaluation. In addition, we showed that PHMG exposure caused lung 
      tumors and genetic alterations according to PHMG exposure under the guidance of 
      CT.
FAU - Kim, Cherry
AU  - Kim C
AD  - Department of Radiology, Ansan Hospital, Korea University College of Medicine, 
      123, Jeokgeum-ro, Danwon-gu, Ansan-si, Gyeonggi, 15355, South Korea.
FAU - Jeong, Sang Hoon
AU  - Jeong SH
AD  - Research Institute for Skin Image, Korea University College of Medicine, 123, 
      Jeokgeum-ro, Danwon-gu, Ansan-si, Gyeonggi, 15355, South Korea.
AD  - Medical Science Research Center, Ansan Hospital, Korea University College of 
      Medicine, 123, Jeokgeum-ro, Danwon-gu, Ansan-si, Gyeonggi, 15355, South Korea.
FAU - Kim, Jaeyoung
AU  - Kim J
AD  - Research Institute for Skin Image, Korea University College of Medicine, 123, 
      Jeokgeum-ro, Danwon-gu, Ansan-si, Gyeonggi, 15355, South Korea.
AD  - Medical Science Research Center, Ansan Hospital, Korea University College of 
      Medicine, 123, Jeokgeum-ro, Danwon-gu, Ansan-si, Gyeonggi, 15355, South Korea.
FAU - Lee, Ki Yeol
AU  - Lee KY
AD  - Department of Radiology, Ansan Hospital, Korea University College of Medicine, 
      123, Jeokgeum-ro, Danwon-gu, Ansan-si, Gyeonggi, 15355, South Korea.
FAU - Cha, Jaehyung
AU  - Cha J
AD  - Medical Science Research Center, Ansan Hospital, Korea University College of 
      Medicine, 123, Jeokgeum-ro, Danwon-gu, Ansan-si, Gyeonggi, 15355, South Korea.
FAU - Lee, Chang Hyun
AU  - Lee CH
AD  - Department of Radiology, College of Medicine, Seoul National University, Seoul 
      National University Hospital, Seoul, 03080, South Korea.
FAU - Park, Eun-Kee
AU  - Park EK
AD  - Department of Medical Humanities and Social Medicine, College of Medicine, Kosin 
      University, Busan, 49267, South Korea.
FAU - Lee, Ju-Han
AU  - Lee JH
AD  - Department of Pathology, Ansan Hospital, Korea University College of Medicine, 
      123, Jeokgeum-ro, Danwon-gu, Ansan-si, Gyeonggi, 15355, South Korea. 
      repath@korea.ac.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210318
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Guanidines)
RN  - 31961-54-3 (polyhexamethyleneguanidine)
SB  - IM
MH  - Animals
MH  - Disease Models, Animal
MH  - Guanidines/toxicity
MH  - Humans
MH  - Lung/*diagnostic imaging/metabolism/pathology
MH  - Lung Injury/chemically induced/*diagnostic imaging/genetics/pathology
MH  - Pulmonary Fibrosis/diagnosis/*diagnostic imaging/genetics/pathology
MH  - Rats
MH  - Sequence Analysis, RNA
MH  - Thorax/*diagnostic imaging/pathology
MH  - Tomography, X-Ray Computed
PMC - PMC7973781
COIS- The authors declare no competing interests.
EDAT- 2021/03/20 06:00
MHDA- 2021/10/12 06:00
PMCR- 2021/03/18
CRDT- 2021/03/19 06:54
PHST- 2020/10/24 00:00 [received]
PHST- 2021/03/04 00:00 [accepted]
PHST- 2021/03/19 06:54 [entrez]
PHST- 2021/03/20 06:00 [pubmed]
PHST- 2021/10/12 06:00 [medline]
PHST- 2021/03/18 00:00 [pmc-release]
AID - 10.1038/s41598-021-85662-z [pii]
AID - 85662 [pii]
AID - 10.1038/s41598-021-85662-z [doi]
PST - epublish
SO  - Sci Rep. 2021 Mar 18;11(1):6318. doi: 10.1038/s41598-021-85662-z.